BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_P01 (897 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.17 SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.68 SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce... 26 6.3 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 8.4 SPCC1281.05 |rsc7||RSC complex subunit Rsc7|Schizosaccharomyces ... 26 8.4 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 31.5 bits (68), Expect = 0.17 Identities = 21/62 (33%), Positives = 28/62 (45%) Frame = +2 Query: 497 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTPS 676 VTP P S S PP +T T S P +++ S T + T +ST P TP+ Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNST--TPVTPT 358 Query: 677 XP 682 P Sbjct: 359 VP 360 Score = 31.5 bits (68), Expect = 0.17 Identities = 21/62 (33%), Positives = 28/62 (45%) Frame = +2 Query: 497 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTPS 676 VTP P S S PP +T T S P +++ S T + T +ST P TP+ Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNST--TPVTPT 412 Query: 677 XP 682 P Sbjct: 413 VP 414 Score = 30.7 bits (66), Expect = 0.29 Identities = 21/63 (33%), Positives = 29/63 (46%), Gaps = 1/63 (1%) Frame = +2 Query: 497 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTP 673 +TP P S S+P P T+ + S P T ST S T + SS++ P TP Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249 Query: 674 SXP 682 + P Sbjct: 250 TVP 252 Score = 28.7 bits (61), Expect = 1.2 Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 1/63 (1%) Frame = +2 Query: 497 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTP 673 +TP P S S+P P T+ + S P T ST S T + T +ST P TP Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPPTGNST--TPVTP 303 Query: 674 SXP 682 + P Sbjct: 304 TVP 306 >SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 29.5 bits (63), Expect = 0.68 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = -1 Query: 579 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 478 DLE N +V Q+ G G+FL G FG + FV+ Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444 >SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1418 Score = 26.2 bits (55), Expect = 6.3 Identities = 15/67 (22%), Positives = 34/67 (50%) Frame = +1 Query: 409 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 588 + T K +N+++H+ L L ++ FG S D +S ++ + +N YF ++ + Sbjct: 146 SSDTNKKVNRKEHNELSLSHLSFNDTSDFG-SSDLSSSEIE---STENDNKAPYFSLLYS 201 Query: 589 EDXQYLK 609 + ++K Sbjct: 202 DGFDFIK 208 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 25.8 bits (54), Expect = 8.4 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +1 Query: 427 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 519 L+ K+D A K+ DQ H K+ +DK S Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526 >SPCC1281.05 |rsc7||RSC complex subunit Rsc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 390 Score = 25.8 bits (54), Expect = 8.4 Identities = 12/43 (27%), Positives = 21/43 (48%) Frame = -2 Query: 134 NNTARTKANKGRRRXGAXXSFESKATNTQKYVRRPERXGRXXR 6 N+T +K +GR G+ ++ S + ++Y R R R R Sbjct: 14 NHTVSSKKRRGRPPKGSYAAYGSSDDDDEEYSGRTRRRNRNTR 56 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,531,631 Number of Sequences: 5004 Number of extensions: 44987 Number of successful extensions: 158 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 156 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 452494940 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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