BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_O14 (906 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model... 29 5.6 At3g11390.1 68416.m01387 DC1 domain-containing protein contains ... 28 9.8 >At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model reflects the alignment of a full-length cDNA sequence gi:18496702. There are multiple frame shifts in the gene model resulting in a truncated protein. The alternate model includes modifications in exons 14, 17 and 29 to compensate for frame shifts and maximize the protein length. It is not based on EST data. adapter protein SPIKE1 [Arabidopsis thaliana] GI:18496703 Length = 1757 Score = 28.7 bits (61), Expect = 5.6 Identities = 20/71 (28%), Positives = 34/71 (47%) Frame = -2 Query: 320 RNSFLWNIDHVTAHCTICLLKTTMFQYQGSDSYNCTHYSKRYYAIDSVLACLTFFFHRIY 141 RN +W+ DHV+A IC + + F + S + Y +DS + L +++ Sbjct: 1385 RNDGVWSKDHVSALRKICPMVSGEFTTEAS-AAEVEGYGASKLTVDSAVKYLQ-LANKL- 1441 Query: 140 SFSKGQSEHFC 108 FS+ + HFC Sbjct: 1442 -FSQAELYHFC 1451 >At3g11390.1 68416.m01387 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 710 Score = 27.9 bits (59), Expect = 9.8 Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 8/85 (9%) Frame = -2 Query: 281 HCTICLLKTTMFQYQGSDSYNCTHYSKRYYAID-SVLACLTFFFHR-------IYSFSKG 126 +C++C K+T F Y+ S++ +C + ID + L +F H I + SKG Sbjct: 490 NCSVCSRKSTGFMYRCSET-DCRLLKDQVLQIDVRCILVLDYFTHESHEHPLFICTSSKG 548 Query: 125 QSEHFCD*LIC*KQLFFKFYLYCIR 51 +++ C+ C K++ + YL C R Sbjct: 549 ENKICCE--GC-KEICQQPYLQCTR 570 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,811,391 Number of Sequences: 28952 Number of extensions: 189915 Number of successful extensions: 288 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 271 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 288 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2139598560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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