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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_O07
         (1088 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    31   0.37 
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    29   1.1  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    29   1.5  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   2.0  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    27   6.1  

>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 30.7 bits (66), Expect = 0.37
 Identities = 22/79 (27%), Positives = 24/79 (30%)
 Frame = +3

Query: 684 SLGXSLPLIPXXXXPPXAPXXXPXXRHXSXPXTPPXXLXXXXPPIXXPF*LXPXXYPPXX 863
           S   S P  P    P  +    P   + S   TPP       PP   P    P   PP  
Sbjct: 382 SRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPP--SLPPSAPPSL 439

Query: 864 RXXPXXXPPXGPXXPDPPP 920
                  PP  P  P  PP
Sbjct: 440 PMGAPAAPPLPPSAPIAPP 458



 Score = 29.5 bits (63), Expect = 0.86
 Identities = 21/73 (28%), Positives = 27/73 (36%), Gaps = 2/73 (2%)
 Frame = +1

Query: 592 SXAXRXPXXSTXKAPXXPPXRXHPTPRXP--SXPSXXPSXSYPXSXYLXXPPPXXXPPXT 765
           S   + P  S+ +A   PP    P P  P  S P+  P  +   +     P P   PP  
Sbjct: 371 STGRQPPPLSSSRAVSNPPA---PPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSA 427

Query: 766 PQXPXPLLPXSXP 804
           P    P  P S P
Sbjct: 428 PPSLPPSAPPSLP 440



 Score = 27.9 bits (59), Expect = 2.6
 Identities = 27/95 (28%), Positives = 31/95 (32%), Gaps = 8/95 (8%)
 Frame = +3

Query: 660 SNSQAPVXSL-GXSLPLIPXXXX-------PPXAPXXXPXXRHXSXPXTPPXXLXXXXPP 815
           SN  AP  ++ G S P +P           P   P   P     S P + P  L    P 
Sbjct: 386 SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPA 445

Query: 816 IXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDPPP 920
              P        PP     P   PP  P  P PPP
Sbjct: 446 AP-PLPPSAPIAPPLPAGMPAA-PPLPPAAPAPPP 478



 Score = 27.5 bits (58), Expect = 3.5
 Identities = 22/75 (29%), Positives = 25/75 (33%)
 Frame = +1

Query: 634 PXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXPLLPXSXPXXX 813
           P  P  R  P P  P           P S       P   PP  P    P LP   P   
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALP---PLGN 408

Query: 814 QSXXRSNSLPXXTPP 858
            S  R+++ P  TPP
Sbjct: 409 AS--RTSTPPVPTPP 421


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 29.1 bits (62), Expect = 1.1
 Identities = 37/160 (23%), Positives = 47/160 (29%), Gaps = 12/160 (7%)
 Frame = +3

Query: 471  PRIXPXTNAVGXLDSXXTXPQRXXPXXSSLXXXXNXXPGX-LXRHXXSXXFHXKSPRXXX 647
            P I P       L S  + P +  P  + L    +  P   L R         K+P    
Sbjct: 967  PSIPPPLPVSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPL 1026

Query: 648  XTXSSN-----SQAPVXSLGXSLPLIPXXXX-PPXAPXXXPXXRHX--SXPX---TPPXX 794
             +  +      S AP   +  S P +P      P AP   P       S P     PP  
Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVP 1086

Query: 795  LXXXXPPIXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDP 914
                 PP+  P    P    P     P   P   P  P P
Sbjct: 1087 APSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP 1126



 Score = 26.2 bits (55), Expect = 8.0
 Identities = 21/78 (26%), Positives = 25/78 (32%), Gaps = 8/78 (10%)
 Frame = +1

Query: 583  PXXSXAXRXPXXSTXKAPXXPPXRXHPTPRX-------PSXPSXXPSXSYPXSXYLXXPP 741
            P  S A   P  S    P   P    P P+        P+  S  P    P +     PP
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193

Query: 742  PXXXPP-XTPQXPXPLLP 792
            P   PP   P    P +P
Sbjct: 1194 PSEAPPVPKPSVGVPPVP 1211


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 28.7 bits (61), Expect = 1.5
 Identities = 25/98 (25%), Positives = 29/98 (29%), Gaps = 3/98 (3%)
 Frame = +3

Query: 633 PRXXXXTXSSNSQAPVXSLGXSLP---LIPXXXXPPXAPXXXPXXRHXSXPXTPPXXLXX 803
           P+      SS S AP  +     P   L P    PP      P         +    +  
Sbjct: 110 PKLRHIGKSSASAAPPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPS 169

Query: 804 XXPPIXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDPP 917
             PP   P    P   PP     P   PP  P  P PP
Sbjct: 170 SLPPPAQP--AAPVKSPPSAPSLPSAVPPMPPKVPPPP 205



 Score = 27.9 bits (59), Expect = 2.6
 Identities = 21/69 (30%), Positives = 24/69 (34%)
 Frame = +1

Query: 571 RTXXPXXSXAXRXPXXSTXKAPXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXXPPPXX 750
           R   P  S A   P  S  KAP  P     P P  P+ P   P  +      +   PP  
Sbjct: 146 RPSIPPPSPASAPPIPS--KAPPIPSSL--PPPAQPAAPVKSPPSAPSLPSAVPPMPPKV 201

Query: 751 XPPXTPQXP 777
            PP   Q P
Sbjct: 202 PPPPLSQAP 210


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 28.3 bits (60), Expect = 2.0
 Identities = 23/94 (24%), Positives = 28/94 (29%), Gaps = 2/94 (2%)
 Frame = +1

Query: 583  PXXSXAXRXPXXSTXKAPXX-PPXRXHPT-PRXPSXPSXXPSXSYPXSXYLXXPPPXXXP 756
            P  +     P   +  AP   PP   H   P  P  P    +   P S      P    P
Sbjct: 1427 PLKASQPTNPGAPSNHAPQVVPPAPMHAVAPVQPKAPGMVTNAPAPSSAPAPPAPVSQLP 1486

Query: 757  PXTPQXPXPLLPXSXPXXXQSXXRSNSLPXXTPP 858
            P  P  P P +  S      S     + P  T P
Sbjct: 1487 PAVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLP 1520



 Score = 27.1 bits (57), Expect = 4.6
 Identities = 19/64 (29%), Positives = 22/64 (34%), Gaps = 2/64 (3%)
 Frame = +1

Query: 658  HPTPRXPSXP--SXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXPLLPXSXPXXXQSXXRS 831
            HP    P  P  +  P  S P       PPP   PP     P P  P S P        +
Sbjct: 1685 HPVSTPPVRPQSAAPPQMSAPTPP----PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSA 1740

Query: 832  NSLP 843
             S+P
Sbjct: 1741 PSVP 1744



 Score = 26.2 bits (55), Expect = 8.0
 Identities = 18/76 (23%), Positives = 21/76 (27%)
 Frame = +1

Query: 556  LXPXSRTXXPXXSXAXRXPXXSTXKAPXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXX 735
            + P      P        P   +   P  PP    P P  P  P+  PS           
Sbjct: 1681 MAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPS----------A 1730

Query: 736  PPPXXXPPXTPQXPXP 783
            PPP       P  P P
Sbjct: 1731 PPPPLPASSAPSVPNP 1746


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 26.6 bits (56), Expect = 6.1
 Identities = 15/53 (28%), Positives = 18/53 (33%), Gaps = 1/53 (1%)
 Frame = +1

Query: 628 KAPXXPPXRXHPT-PRXPSXPSXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXP 783
           +AP  P     P  P  PS P        P +  +  PP     P  P  P P
Sbjct: 630 EAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQP 682


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,473,722
Number of Sequences: 5004
Number of extensions: 12898
Number of successful extensions: 59
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 573748416
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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