BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_O07 (1088 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.37 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.1 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.5 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.0 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 6.1 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 30.7 bits (66), Expect = 0.37 Identities = 22/79 (27%), Positives = 24/79 (30%) Frame = +3 Query: 684 SLGXSLPLIPXXXXPPXAPXXXPXXRHXSXPXTPPXXLXXXXPPIXXPF*LXPXXYPPXX 863 S S P P P + P + S TPP PP P P PP Sbjct: 382 SRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPP--SLPPSAPPSL 439 Query: 864 RXXPXXXPPXGPXXPDPPP 920 PP P P PP Sbjct: 440 PMGAPAAPPLPPSAPIAPP 458 Score = 29.5 bits (63), Expect = 0.86 Identities = 21/73 (28%), Positives = 27/73 (36%), Gaps = 2/73 (2%) Frame = +1 Query: 592 SXAXRXPXXSTXKAPXXPPXRXHPTPRXP--SXPSXXPSXSYPXSXYLXXPPPXXXPPXT 765 S + P S+ +A PP P P P S P+ P + + P P PP Sbjct: 371 STGRQPPPLSSSRAVSNPPA---PPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSA 427 Query: 766 PQXPXPLLPXSXP 804 P P P S P Sbjct: 428 PPSLPPSAPPSLP 440 Score = 27.9 bits (59), Expect = 2.6 Identities = 27/95 (28%), Positives = 31/95 (32%), Gaps = 8/95 (8%) Frame = +3 Query: 660 SNSQAPVXSL-GXSLPLIPXXXX-------PPXAPXXXPXXRHXSXPXTPPXXLXXXXPP 815 SN AP ++ G S P +P P P P S P + P L P Sbjct: 386 SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPA 445 Query: 816 IXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDPPP 920 P PP P PP P P PPP Sbjct: 446 AP-PLPPSAPIAPPLPAGMPAA-PPLPPAAPAPPP 478 Score = 27.5 bits (58), Expect = 3.5 Identities = 22/75 (29%), Positives = 25/75 (33%) Frame = +1 Query: 634 PXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXPLLPXSXPXXX 813 P P R P P P P S P PP P P LP P Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALP---PLGN 408 Query: 814 QSXXRSNSLPXXTPP 858 S R+++ P TPP Sbjct: 409 AS--RTSTPPVPTPP 421 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.1 bits (62), Expect = 1.1 Identities = 37/160 (23%), Positives = 47/160 (29%), Gaps = 12/160 (7%) Frame = +3 Query: 471 PRIXPXTNAVGXLDSXXTXPQRXXPXXSSLXXXXNXXPGX-LXRHXXSXXFHXKSPRXXX 647 P I P L S + P + P + L + P L R K+P Sbjct: 967 PSIPPPLPVSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPL 1026 Query: 648 XTXSSN-----SQAPVXSLGXSLPLIPXXXX-PPXAPXXXPXXRHX--SXPX---TPPXX 794 + + S AP + S P +P P AP P S P PP Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVP 1086 Query: 795 LXXXXPPIXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDP 914 PP+ P P P P P P P P Sbjct: 1087 APSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP 1126 Score = 26.2 bits (55), Expect = 8.0 Identities = 21/78 (26%), Positives = 25/78 (32%), Gaps = 8/78 (10%) Frame = +1 Query: 583 PXXSXAXRXPXXSTXKAPXXPPXRXHPTPRX-------PSXPSXXPSXSYPXSXYLXXPP 741 P S A P S P P P P+ P+ S P P + PP Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193 Query: 742 PXXXPP-XTPQXPXPLLP 792 P PP P P +P Sbjct: 1194 PSEAPPVPKPSVGVPPVP 1211 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 28.7 bits (61), Expect = 1.5 Identities = 25/98 (25%), Positives = 29/98 (29%), Gaps = 3/98 (3%) Frame = +3 Query: 633 PRXXXXTXSSNSQAPVXSLGXSLP---LIPXXXXPPXAPXXXPXXRHXSXPXTPPXXLXX 803 P+ SS S AP + P L P PP P + + Sbjct: 110 PKLRHIGKSSASAAPPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPS 169 Query: 804 XXPPIXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDPP 917 PP P P PP P PP P P PP Sbjct: 170 SLPPPAQP--AAPVKSPPSAPSLPSAVPPMPPKVPPPP 205 Score = 27.9 bits (59), Expect = 2.6 Identities = 21/69 (30%), Positives = 24/69 (34%) Frame = +1 Query: 571 RTXXPXXSXAXRXPXXSTXKAPXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXXPPPXX 750 R P S A P S KAP P P P P+ P P + + PP Sbjct: 146 RPSIPPPSPASAPPIPS--KAPPIPSSL--PPPAQPAAPVKSPPSAPSLPSAVPPMPPKV 201 Query: 751 XPPXTPQXP 777 PP Q P Sbjct: 202 PPPPLSQAP 210 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.3 bits (60), Expect = 2.0 Identities = 23/94 (24%), Positives = 28/94 (29%), Gaps = 2/94 (2%) Frame = +1 Query: 583 PXXSXAXRXPXXSTXKAPXX-PPXRXHPT-PRXPSXPSXXPSXSYPXSXYLXXPPPXXXP 756 P + P + AP PP H P P P + P S P P Sbjct: 1427 PLKASQPTNPGAPSNHAPQVVPPAPMHAVAPVQPKAPGMVTNAPAPSSAPAPPAPVSQLP 1486 Query: 757 PXTPQXPXPLLPXSXPXXXQSXXRSNSLPXXTPP 858 P P P P + S S + P T P Sbjct: 1487 PAVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLP 1520 Score = 27.1 bits (57), Expect = 4.6 Identities = 19/64 (29%), Positives = 22/64 (34%), Gaps = 2/64 (3%) Frame = +1 Query: 658 HPTPRXPSXP--SXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXPLLPXSXPXXXQSXXRS 831 HP P P + P S P PPP PP P P P S P + Sbjct: 1685 HPVSTPPVRPQSAAPPQMSAPTPP----PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSA 1740 Query: 832 NSLP 843 S+P Sbjct: 1741 PSVP 1744 Score = 26.2 bits (55), Expect = 8.0 Identities = 18/76 (23%), Positives = 21/76 (27%) Frame = +1 Query: 556 LXPXSRTXXPXXSXAXRXPXXSTXKAPXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXX 735 + P P P + P PP P P P P+ PS Sbjct: 1681 MAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPS----------A 1730 Query: 736 PPPXXXPPXTPQXPXP 783 PPP P P P Sbjct: 1731 PPPPLPASSAPSVPNP 1746 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 26.6 bits (56), Expect = 6.1 Identities = 15/53 (28%), Positives = 18/53 (33%), Gaps = 1/53 (1%) Frame = +1 Query: 628 KAPXXPPXRXHPT-PRXPSXPSXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXP 783 +AP P P P PS P P + + PP P P P P Sbjct: 630 EAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQP 682 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,473,722 Number of Sequences: 5004 Number of extensions: 12898 Number of successful extensions: 59 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 21 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 49 length of database: 2,362,478 effective HSP length: 74 effective length of database: 1,992,182 effective search space used: 573748416 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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