BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_O07
(1088 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.37
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.1
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.0
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 6.1
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.7 bits (66), Expect = 0.37
Identities = 22/79 (27%), Positives = 24/79 (30%)
Frame = +3
Query: 684 SLGXSLPLIPXXXXPPXAPXXXPXXRHXSXPXTPPXXLXXXXPPIXXPF*LXPXXYPPXX 863
S S P P P + P + S TPP PP P P PP
Sbjct: 382 SRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPP--SLPPSAPPSL 439
Query: 864 RXXPXXXPPXGPXXPDPPP 920
PP P P PP
Sbjct: 440 PMGAPAAPPLPPSAPIAPP 458
Score = 29.5 bits (63), Expect = 0.86
Identities = 21/73 (28%), Positives = 27/73 (36%), Gaps = 2/73 (2%)
Frame = +1
Query: 592 SXAXRXPXXSTXKAPXXPPXRXHPTPRXP--SXPSXXPSXSYPXSXYLXXPPPXXXPPXT 765
S + P S+ +A PP P P P S P+ P + + P P PP
Sbjct: 371 STGRQPPPLSSSRAVSNPPA---PPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSA 427
Query: 766 PQXPXPLLPXSXP 804
P P P S P
Sbjct: 428 PPSLPPSAPPSLP 440
Score = 27.9 bits (59), Expect = 2.6
Identities = 27/95 (28%), Positives = 31/95 (32%), Gaps = 8/95 (8%)
Frame = +3
Query: 660 SNSQAPVXSL-GXSLPLIPXXXX-------PPXAPXXXPXXRHXSXPXTPPXXLXXXXPP 815
SN AP ++ G S P +P P P P S P + P L P
Sbjct: 386 SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPA 445
Query: 816 IXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDPPP 920
P PP P PP P P PPP
Sbjct: 446 AP-PLPPSAPIAPPLPAGMPAA-PPLPPAAPAPPP 478
Score = 27.5 bits (58), Expect = 3.5
Identities = 22/75 (29%), Positives = 25/75 (33%)
Frame = +1
Query: 634 PXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXPLLPXSXPXXX 813
P P R P P P P S P PP P P LP P
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALP---PLGN 408
Query: 814 QSXXRSNSLPXXTPP 858
S R+++ P TPP
Sbjct: 409 AS--RTSTPPVPTPP 421
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 1.1
Identities = 37/160 (23%), Positives = 47/160 (29%), Gaps = 12/160 (7%)
Frame = +3
Query: 471 PRIXPXTNAVGXLDSXXTXPQRXXPXXSSLXXXXNXXPGX-LXRHXXSXXFHXKSPRXXX 647
P I P L S + P + P + L + P L R K+P
Sbjct: 967 PSIPPPLPVSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPL 1026
Query: 648 XTXSSN-----SQAPVXSLGXSLPLIPXXXX-PPXAPXXXPXXRHX--SXPX---TPPXX 794
+ + S AP + S P +P P AP P S P PP
Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVP 1086
Query: 795 LXXXXPPIXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDP 914
PP+ P P P P P P P P
Sbjct: 1087 APSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP 1126
Score = 26.2 bits (55), Expect = 8.0
Identities = 21/78 (26%), Positives = 25/78 (32%), Gaps = 8/78 (10%)
Frame = +1
Query: 583 PXXSXAXRXPXXSTXKAPXXPPXRXHPTPRX-------PSXPSXXPSXSYPXSXYLXXPP 741
P S A P S P P P P+ P+ S P P + PP
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193
Query: 742 PXXXPP-XTPQXPXPLLP 792
P PP P P +P
Sbjct: 1194 PSEAPPVPKPSVGVPPVP 1211
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.7 bits (61), Expect = 1.5
Identities = 25/98 (25%), Positives = 29/98 (29%), Gaps = 3/98 (3%)
Frame = +3
Query: 633 PRXXXXTXSSNSQAPVXSLGXSLP---LIPXXXXPPXAPXXXPXXRHXSXPXTPPXXLXX 803
P+ SS S AP + P L P PP P + +
Sbjct: 110 PKLRHIGKSSASAAPPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPS 169
Query: 804 XXPPIXXPF*LXPXXYPPXXRXXPXXXPPXGPXXPDPP 917
PP P P PP P PP P P PP
Sbjct: 170 SLPPPAQP--AAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
Score = 27.9 bits (59), Expect = 2.6
Identities = 21/69 (30%), Positives = 24/69 (34%)
Frame = +1
Query: 571 RTXXPXXSXAXRXPXXSTXKAPXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXXPPPXX 750
R P S A P S KAP P P P P+ P P + + PP
Sbjct: 146 RPSIPPPSPASAPPIPS--KAPPIPSSL--PPPAQPAAPVKSPPSAPSLPSAVPPMPPKV 201
Query: 751 XPPXTPQXP 777
PP Q P
Sbjct: 202 PPPPLSQAP 210
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 2.0
Identities = 23/94 (24%), Positives = 28/94 (29%), Gaps = 2/94 (2%)
Frame = +1
Query: 583 PXXSXAXRXPXXSTXKAPXX-PPXRXHPT-PRXPSXPSXXPSXSYPXSXYLXXPPPXXXP 756
P + P + AP PP H P P P + P S P P
Sbjct: 1427 PLKASQPTNPGAPSNHAPQVVPPAPMHAVAPVQPKAPGMVTNAPAPSSAPAPPAPVSQLP 1486
Query: 757 PXTPQXPXPLLPXSXPXXXQSXXRSNSLPXXTPP 858
P P P P + S S + P T P
Sbjct: 1487 PAVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLP 1520
Score = 27.1 bits (57), Expect = 4.6
Identities = 19/64 (29%), Positives = 22/64 (34%), Gaps = 2/64 (3%)
Frame = +1
Query: 658 HPTPRXPSXP--SXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXPLLPXSXPXXXQSXXRS 831
HP P P + P S P PPP PP P P P S P +
Sbjct: 1685 HPVSTPPVRPQSAAPPQMSAPTPP----PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSA 1740
Query: 832 NSLP 843
S+P
Sbjct: 1741 PSVP 1744
Score = 26.2 bits (55), Expect = 8.0
Identities = 18/76 (23%), Positives = 21/76 (27%)
Frame = +1
Query: 556 LXPXSRTXXPXXSXAXRXPXXSTXKAPXXPPXRXHPTPRXPSXPSXXPSXSYPXSXYLXX 735
+ P P P + P PP P P P P+ PS
Sbjct: 1681 MAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPS----------A 1730
Query: 736 PPPXXXPPXTPQXPXP 783
PPP P P P
Sbjct: 1731 PPPPLPASSAPSVPNP 1746
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.6 bits (56), Expect = 6.1
Identities = 15/53 (28%), Positives = 18/53 (33%), Gaps = 1/53 (1%)
Frame = +1
Query: 628 KAPXXPPXRXHPT-PRXPSXPSXXPSXSYPXSXYLXXPPPXXXPPXTPQXPXP 783
+AP P P P PS P P + + PP P P P P
Sbjct: 630 EAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQP 682
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,473,722
Number of Sequences: 5004
Number of extensions: 12898
Number of successful extensions: 59
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 573748416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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