Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_N04
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 77 3e-15
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 33 0.054
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 31 0.29
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 30 0.38
SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces ... 29 0.88
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 29 0.88
SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit ... 29 1.2
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.5
SPBC25B2.03 |||zf-C3HC4 type zinc finger|Schizosaccharomyces pom... 28 2.0
SPBC26H8.13c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 28 2.0
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 6.2
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 6.2
SPAC3F10.05c |mug113||DUF1766 family protein|Schizosaccharomyces... 26 6.2
SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc... 26 8.2
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 77.0 bits (181), Expect = 3e-15
Identities = 32/75 (42%), Positives = 45/75 (60%), Gaps = 4/75 (5%)
Frame = +2
Query: 548 HMRRVHERFFCDLCVNHLKIFTNERRCYTRQELAHHRRKG----DLDDTSHRGHPLCXFC 715
H + H +FFCDLCV + K+FT+E ++++ L H G DL+ T +GHP C FC
Sbjct: 191 HAKVKHHKFFCDLCVKNKKVFTHEHTLFSKKGLTKHNEVGDQGSDLEITGFKGHPKCEFC 250
Query: 716 EXRFMDPDELYRHLR 760
F D DEL++H R
Sbjct: 251 NTHFYDDDELFKHCR 265
Score = 45.2 bits (102), Expect = 1e-05
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +2
Query: 188 EAQANENTCVVCFKNVLYYSIGECDHPVCFECSTRMRVLCLQNECPICRKDLLRVVFT 361
E +E C +C + + Y + C+H +C C+ R+R L EC C+ + V+ T
Sbjct: 73 EQDEDEQICFICAEGITYSCVLPCNHRMCHVCALRLRALYKTKECTFCKTEWDTVLIT 130
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 33.1 bits (72), Expect = 0.054
Identities = 18/66 (27%), Positives = 28/66 (42%)
Frame = +2
Query: 137 YRNAFTES*INNTTIMSEAQANENTCVVCFKNVLYYSIGECDHPVCFECSTRMRVLCLQN 316
Y A E ++ ++ E + E C +CF + + C H C C M+ L
Sbjct: 145 YETAVAEF-LHMEDLLQENVSRELECQICFGMLYDPVVSPCGHTFCGPC--LMQALTQSP 201
Query: 317 ECPICR 334
+CP CR
Sbjct: 202 QCPTCR 207
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 30.7 bits (66), Expect = 0.29
Identities = 12/33 (36%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +2
Query: 188 EAQANENTCVVCF-KNVLYYSIGECDHPVCFEC 283
E +E TC +C+ + L + ECDH C C
Sbjct: 123 EVVHHEGTCEICYDEGCLPFFSAECDHEFCLAC 155
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 30.3 bits (65), Expect = 0.38
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = -2
Query: 256 LAN*VVQNVLETNYASILVRLSLRHDCCI 170
LA VVQNVL N +S+LV+ + R+ CI
Sbjct: 312 LAEPVVQNVLWVNTSSVLVQFTNRNSTCI 340
>SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 496
Score = 29.1 bits (62), Expect = 0.88
Identities = 18/66 (27%), Positives = 26/66 (39%), Gaps = 1/66 (1%)
Frame = +2
Query: 371 HPYKELGNRXIPXID-CLKDNTKLYSVLKT*KMHMKTCWRIGVKSVIRYHRSATLXLLND 547
H +E+G + C DN K+Y+V K H +C GV + L N
Sbjct: 11 HIGREIGTGAFASVRLCYDDNAKIYAVKFVNKKHATSCMNAGVWARRMASEIQLHKLCNG 70
Query: 548 HMRRVH 565
H +H
Sbjct: 71 HKNIIH 76
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 29.1 bits (62), Expect = 0.88
Identities = 13/54 (24%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
Frame = +2
Query: 179 IMSEAQANENTCVVCFKNVLYYSIGECDHPVCFEC-STRMRVLCLQNECPICRK 337
+ S + + CV+C + S C H C C + + +CP+CR+
Sbjct: 195 VPSSQRLADYKCVICLDSPENLSCTPCGHIFCNFCILSALGTTAATQKCPVCRR 248
>SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit
Rrn6 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 868
Score = 28.7 bits (61), Expect = 1.2
Identities = 29/107 (27%), Positives = 52/107 (48%), Gaps = 4/107 (3%)
Frame = +2
Query: 146 AFTES*INNTTIMSEAQANENTCVVCFKN---VLYYSIGEC-DHPVCFECSTRMRVLCLQ 313
A+ E IN + A+ E V N +L+ S+GE D + F CS+ +R+L +
Sbjct: 334 AYHEEEINPDNTIYRAKWEEYFGGVILHNAFFILHVSLGEVPDFHLLFHCSSDVRILQVV 393
Query: 314 NECPICRKDLLRVVFTDSIHPYKELGNRXIPXIDCLKDNTKLYSVLK 454
NE + + ++ T+S+ + ++ + P ++ K N KL LK
Sbjct: 394 NESMPIKSEFF-ILTTESV-LWMDIQHPQKPLLE-WKHNRKLDPTLK 437
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/44 (29%), Positives = 19/44 (43%)
Frame = +2
Query: 212 CVVCFKNVLYYSIGECDHPVCFECSTRMRVLCLQNECPICRKDL 343
C +C + + C H C+EC + L CP CR+ L
Sbjct: 85 CPICTEALQRPFTTHCGHTYCYEC--LLNWLKESKSCPTCRQKL 126
>SPBC25B2.03 |||zf-C3HC4 type zinc finger|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 554
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 5/47 (10%)
Frame = +2
Query: 173 TTIMSEAQANENTCVVCFKNVLYYSIGE-----CDHPVCFECSTRMR 298
TT ++ AN C +CF LYY C P+C EC +R
Sbjct: 138 TTAITTIYANAMECPICF---LYYPSNFNYTRCCAQPICSECFVEIR 181
>SPBC26H8.13c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 124
Score = 27.9 bits (59), Expect = 2.0
Identities = 18/79 (22%), Positives = 31/79 (39%), Gaps = 2/79 (2%)
Frame = +2
Query: 101 ASTYSQFNKTLGYRNAFTES*INNTTIMSEA--QANENTCVVCFKNVLYYSIGECDHPVC 274
A+ F++ G+R NN + ++TC +C + +C+ VC
Sbjct: 45 AAVKEAFSENRGFRTCHVCHRKNNMRLKVGTCESCKKHTCAICIRQC-----HKCESNVC 99
Query: 275 FECSTRMRVLCLQNECPIC 331
CS + R + CP C
Sbjct: 100 SMCSKQERTFESEAFCPSC 118
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 26.2 bits (55), Expect = 6.2
Identities = 14/48 (29%), Positives = 22/48 (45%), Gaps = 4/48 (8%)
Frame = +2
Query: 203 ENTCVVCFKNVLYYS----IGECDHPVCFECSTRMRVLCLQNECPICR 334
+ C+VC N + +C+H EC + + QN CP+CR
Sbjct: 522 DERCLVCLSNFELNDECRRLKQCNHFFHRECIDQW-LTSSQNSCPLCR 568
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 26.2 bits (55), Expect = 6.2
Identities = 9/27 (33%), Positives = 12/27 (44%)
Frame = +2
Query: 254 ECDHPVCFECSTRMRVLCLQNECPICR 334
+C H C+ C L CPIC+
Sbjct: 212 DCGHTYCYACIMSRLKLVNNVSCPICK 238
>SPAC3F10.05c |mug113||DUF1766 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 326
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -2
Query: 772 TSAPAQVTVQLVRIHEPXLTEXTKWMAP 689
TS P QV+ ++ R+ L E WM P
Sbjct: 259 TSKPCQVSFKVERLVHKELNEYLSWMQP 286
>SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 434
Score = 25.8 bits (54), Expect = 8.2
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 6/45 (13%)
Frame = -1
Query: 521 NGGILSQILHRFSN------KFSYAFFMSSEQNTILYCLSNSLXR 405
NGGI+ Q+L SN KF+ FF++ + N Y + N + R
Sbjct: 91 NGGIVIQVLGEMSNKGKLSRKFAQTFFLAEQPNG--YFVLNDIFR 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,559,593
Number of Sequences: 5004
Number of extensions: 73105
Number of successful extensions: 235
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 234
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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