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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_M12
         (920 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC) si...   113   1e-25
At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA) si...   113   1e-25
At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB) id...   112   4e-25
At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD) ri...   111   5e-25
At4g11860.1 68417.m01887 expressed protein contains Pfam domain ...    29   3.3  
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil...    28   7.6  
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil...    28   7.6  

>At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC)
           similar to ribosomal protein L33B GB:NP_014877 from
           [Saccharomyces cerevisiae]
          Length = 112

 Score =  113 bits (273), Expect = 1e-25
 Identities = 53/110 (48%), Positives = 70/110 (63%)
 Frame = +1

Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402
           RLY +    GYKR   NQ+ NT+L+++EG   + +  +Y GK   Y+Y+AK +       
Sbjct: 11  RLYVRGTILGYKRSKSNQYPNTSLVQIEGVNTQEEVNWYKGKRMAYIYKAKTKK------ 64

Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552
               +  R IWGKVTRPHGNSG VRAKF SNLP ++MG R+RV +YPS I
Sbjct: 65  --NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMGSRVRVFMYPSNI 112


>At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA)
           similar to ribosomal protein L35a GI:57118 from [Rattus
           norvegicus]
          Length = 112

 Score =  113 bits (273), Expect = 1e-25
 Identities = 54/110 (49%), Positives = 69/110 (62%)
 Frame = +1

Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402
           RLY +    GYKR   NQ+ NT+L++VEG     +  +Y GK   Y+Y+AK +       
Sbjct: 11  RLYVRGTILGYKRSKSNQYPNTSLVQVEGVNTTEEVSWYKGKRMAYIYKAKTKK------ 64

Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552
               +  R IWGKVTRPHGNSG VRAKF SNLP ++MG R+RV +YPS I
Sbjct: 65  --NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMGSRVRVFMYPSNI 112


>At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB)
           identical to GB:CAB81600 from [Arabidopsis thaliana]
          Length = 111

 Score =  112 bits (269), Expect = 4e-25
 Identities = 52/110 (47%), Positives = 70/110 (63%)
 Frame = +1

Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402
           RLY +    GYKR   NQ+ NT+L+++EG   + +  +Y GK   Y+Y+AK +       
Sbjct: 10  RLYVRGTVLGYKRSKSNQYPNTSLIQIEGVNTQEEVNWYKGKRLAYIYKAKTKK------ 63

Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552
               +  R IWGKVTRPHGNSG VR+KF SNLP ++MG R+RV +YPS I
Sbjct: 64  --NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMGARVRVFMYPSNI 111


>At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD)
           ribosomal protein L35a.e.c15, Saccharomyces cerevisiae,
           PIR:S44069
          Length = 111

 Score =  111 bits (268), Expect = 5e-25
 Identities = 52/110 (47%), Positives = 70/110 (63%)
 Frame = +1

Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402
           RLY +    GYKR   NQ+ NT+L+++EG   + +  +Y GK   Y+Y+AK +       
Sbjct: 10  RLYVRGTVLGYKRSKSNQYPNTSLVQIEGVNTQEEVNWYKGKRLAYIYKAKTKK------ 63

Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552
               +  R IWGKVTRPHGNSG VR+KF SNLP ++MG R+RV +YPS I
Sbjct: 64  --NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMGARVRVFMYPSNI 111


>At4g11860.1 68417.m01887 expressed protein contains Pfam domain
           PF04424: Protein of unknown function (DUF544)
          Length = 682

 Score = 29.5 bits (63), Expect = 3.3
 Identities = 14/62 (22%), Positives = 29/62 (46%)
 Frame = +1

Query: 304 EGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAK 483
           EG K+R   VF+   H   +++ +    +    +G   +   +W K+   +G++  + A 
Sbjct: 508 EGLKERELCVFFRNNHFCTMFKYEGELYLLATDQGYLNQPDLVWEKLNEVNGDTAFMTAT 567

Query: 484 FK 489
           FK
Sbjct: 568 FK 569


>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS8) similar to LACS 4 [SP|O35547] from Rattus
           norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
           contains Pfam HMM hit: AMP-binding enzymes PF00501
          Length = 720

 Score = 28.3 bits (60), Expect = 7.6
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = -1

Query: 578 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 438
           F+ +  GS I +GY +T       ++   D  + R  P LPCG V L
Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509


>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS8) similar to LACS 4 [SP|O35547] from Rattus
           norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
           contains Pfam HMM hit: AMP-binding enzymes PF00501
          Length = 720

 Score = 28.3 bits (60), Expect = 7.6
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = -1

Query: 578 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 438
           F+ +  GS I +GY +T       ++   D  + R  P LPCG V L
Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,844,037
Number of Sequences: 28952
Number of extensions: 246573
Number of successful extensions: 642
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 638
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2188225800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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