BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_M12 (920 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC) si... 113 1e-25 At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA) si... 113 1e-25 At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB) id... 112 4e-25 At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD) ri... 111 5e-25 At4g11860.1 68417.m01887 expressed protein contains Pfam domain ... 29 3.3 At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 28 7.6 At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 28 7.6 >At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC) similar to ribosomal protein L33B GB:NP_014877 from [Saccharomyces cerevisiae] Length = 112 Score = 113 bits (273), Expect = 1e-25 Identities = 53/110 (48%), Positives = 70/110 (63%) Frame = +1 Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402 RLY + GYKR NQ+ NT+L+++EG + + +Y GK Y+Y+AK + Sbjct: 11 RLYVRGTILGYKRSKSNQYPNTSLVQIEGVNTQEEVNWYKGKRMAYIYKAKTKK------ 64 Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552 + R IWGKVTRPHGNSG VRAKF SNLP ++MG R+RV +YPS I Sbjct: 65 --NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMGSRVRVFMYPSNI 112 >At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA) similar to ribosomal protein L35a GI:57118 from [Rattus norvegicus] Length = 112 Score = 113 bits (273), Expect = 1e-25 Identities = 54/110 (49%), Positives = 69/110 (62%) Frame = +1 Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402 RLY + GYKR NQ+ NT+L++VEG + +Y GK Y+Y+AK + Sbjct: 11 RLYVRGTILGYKRSKSNQYPNTSLVQVEGVNTTEEVSWYKGKRMAYIYKAKTKK------ 64 Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552 + R IWGKVTRPHGNSG VRAKF SNLP ++MG R+RV +YPS I Sbjct: 65 --NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMGSRVRVFMYPSNI 112 >At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB) identical to GB:CAB81600 from [Arabidopsis thaliana] Length = 111 Score = 112 bits (269), Expect = 4e-25 Identities = 52/110 (47%), Positives = 70/110 (63%) Frame = +1 Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402 RLY + GYKR NQ+ NT+L+++EG + + +Y GK Y+Y+AK + Sbjct: 10 RLYVRGTVLGYKRSKSNQYPNTSLIQIEGVNTQEEVNWYKGKRLAYIYKAKTKK------ 63 Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552 + R IWGKVTRPHGNSG VR+KF SNLP ++MG R+RV +YPS I Sbjct: 64 --NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMGARVRVFMYPSNI 111 >At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD) ribosomal protein L35a.e.c15, Saccharomyces cerevisiae, PIR:S44069 Length = 111 Score = 111 bits (268), Expect = 5e-25 Identities = 52/110 (47%), Positives = 70/110 (63%) Frame = +1 Query: 223 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 402 RLY + GYKR NQ+ NT+L+++EG + + +Y GK Y+Y+AK + Sbjct: 10 RLYVRGTVLGYKRSKSNQYPNTSLVQIEGVNTQEEVNWYKGKRLAYIYKAKTKK------ 63 Query: 403 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 552 + R IWGKVTRPHGNSG VR+KF SNLP ++MG R+RV +YPS I Sbjct: 64 --NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMGARVRVFMYPSNI 111 >At4g11860.1 68417.m01887 expressed protein contains Pfam domain PF04424: Protein of unknown function (DUF544) Length = 682 Score = 29.5 bits (63), Expect = 3.3 Identities = 14/62 (22%), Positives = 29/62 (46%) Frame = +1 Query: 304 EGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAK 483 EG K+R VF+ H +++ + + +G + +W K+ +G++ + A Sbjct: 508 EGLKERELCVFFRNNHFCTMFKYEGELYLLATDQGYLNQPDLVWEKLNEVNGDTAFMTAT 567 Query: 484 FK 489 FK Sbjct: 568 FK 569 >At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family protein / long-chain acyl-CoA synthetase family protein (LACS8) similar to LACS 4 [SP|O35547] from Rattus norvegicus, LACS 4 [SP|O60488] from Homo sapiens; contains Pfam HMM hit: AMP-binding enzymes PF00501 Length = 720 Score = 28.3 bits (60), Expect = 7.6 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = -1 Query: 578 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 438 F+ + GS I +GY +T ++ D + R P LPCG V L Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509 >At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family protein / long-chain acyl-CoA synthetase family protein (LACS8) similar to LACS 4 [SP|O35547] from Rattus norvegicus, LACS 4 [SP|O60488] from Homo sapiens; contains Pfam HMM hit: AMP-binding enzymes PF00501 Length = 720 Score = 28.3 bits (60), Expect = 7.6 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = -1 Query: 578 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 438 F+ + GS I +GY +T ++ D + R P LPCG V L Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,844,037 Number of Sequences: 28952 Number of extensions: 246573 Number of successful extensions: 642 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 610 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 638 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2188225800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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