Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_M01
         (857 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P04142 Cluster: Cecropin-B precursor; n=16; Obtectomera...    76   1e-12
UniRef50_P01507 Cluster: Cecropin-A precursor; n=17; Ditrysia|Re...    75   3e-12
UniRef50_A6BMG0 Cluster: Cecropin A; n=1; Plutella xylostella|Re...    57   5e-07
UniRef50_P01511 Cluster: Cecropin-D; n=6; Obtectomera|Rep: Cecro...    46   0.001
UniRef50_Q2WGL2 Cluster: Antibacterial peptide; n=4; Obtectomera...    44   0.005
UniRef50_P48821 Cluster: Antibacterial peptide enbocin precursor...    36   0.99 
UniRef50_UPI00006CB5FA Cluster: hypothetical protein TTHERM_0053...    33   9.2  
UniRef50_Q0Q027 Cluster: Putative defense protein; n=1; Antherae...    33   9.2  

>UniRef50_P04142 Cluster: Cecropin-B precursor; n=16;
           Obtectomera|Rep: Cecropin-B precursor - Bombyx mori
           (Silk moth)
          Length = 63

 Score = 75.8 bits (178), Expect = 1e-12
 Identities = 33/63 (52%), Positives = 42/63 (66%)
 Frame = +3

Query: 123 MNFVRIXXXXXXXXXXXXXXXXXPEPRWKLFKKIEKVGRXVRDGLIKAGPAIAVIGQAKS 302
           MNF +I                 PEPRWK+FKKIEK+GR +RDG++KAGPAI V+G AK+
Sbjct: 1   MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60

Query: 303 LGK 311
           +GK
Sbjct: 61  IGK 63


>UniRef50_P01507 Cluster: Cecropin-A precursor; n=17; Ditrysia|Rep:
           Cecropin-A precursor - Hyalophora cecropia (Cecropia
           moth)
          Length = 64

 Score = 74.5 bits (175), Expect = 3e-12
 Identities = 34/63 (53%), Positives = 41/63 (65%)
 Frame = +3

Query: 123 MNFVRIXXXXXXXXXXXXXXXXXPEPRWKLFKKIEKVGRXVRDGLIKAGPAIAVIGQAKS 302
           MNF RI                 PEP+WKLFKKIEKVG+ +RDG+IKAGPA+AV+GQA  
Sbjct: 1   MNFSRIFFFVFACLTALAMVNAAPEPKWKLFKKIEKVGQNIRDGIIKAGPAVAVVGQATQ 60

Query: 303 LGK 311
           + K
Sbjct: 61  IAK 63


>UniRef50_A6BMG0 Cluster: Cecropin A; n=1; Plutella xylostella|Rep:
           Cecropin A - Plutella xylostella (Diamondback moth)
          Length = 66

 Score = 57.2 bits (132), Expect = 5e-07
 Identities = 26/41 (63%), Positives = 34/41 (82%), Gaps = 1/41 (2%)
 Frame = +3

Query: 198 PRWKLFKKIEKVGRXVRDGLIK-AGPAIAVIGQAKSLGK*T 317
           PRWK FKK+EKVGR +R+G+I+  GPA+AVIGQA S+ + T
Sbjct: 24  PRWKPFKKLEKVGRNIRNGIIRYNGPAVAVIGQATSIARPT 64


>UniRef50_P01511 Cluster: Cecropin-D; n=6; Obtectomera|Rep:
           Cecropin-D - Antheraea pernyi (Chinese oak silk moth)
          Length = 36

 Score = 46.4 bits (105), Expect = 0.001
 Identities = 18/36 (50%), Positives = 26/36 (72%)
 Frame = +3

Query: 204 WKLFKKIEKVGRXVRDGLIKAGPAIAVIGQAKSLGK 311
           W  FK++E+ G+ VRD +I AGPA+A + QA +L K
Sbjct: 1   WNPFKELERAGQRVRDAIISAGPAVATVAQATALAK 36


>UniRef50_Q2WGL2 Cluster: Antibacterial peptide; n=4;
           Obtectomera|Rep: Antibacterial peptide - Bombyx mori
           (Silk moth)
          Length = 66

 Score = 44.0 bits (99), Expect = 0.005
 Identities = 19/34 (55%), Positives = 25/34 (73%)
 Frame = +3

Query: 204 WKLFKKIEKVGRXVRDGLIKAGPAIAVIGQAKSL 305
           W  FK++E VG+ VRD +I AGPAI V+ +AK L
Sbjct: 23  WDFFKELEGVGQRVRDSIISAGPAIDVLQKAKGL 56


>UniRef50_P48821 Cluster: Antibacterial peptide enbocin precursor;
           n=5; Ditrysia|Rep: Antibacterial peptide enbocin
           precursor - Bombyx mori (Silk moth)
          Length = 59

 Score = 36.3 bits (80), Expect = 0.99
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +3

Query: 204 WKLFKKIEKVGRXVRDGLIKAGPAIAVIGQAKSL 305
           W +FK+IE+     RD +I AGPA+  +  A S+
Sbjct: 23  WNIFKEIERAVARTRDAVISAGPAVRTVAAATSV 56


>UniRef50_UPI00006CB5FA Cluster: hypothetical protein TTHERM_00537400;
            n=1; Tetrahymena thermophila SB210|Rep: hypothetical
            protein TTHERM_00537400 - Tetrahymena thermophila SB210
          Length = 2268

 Score = 33.1 bits (72), Expect = 9.2
 Identities = 21/52 (40%), Positives = 29/52 (55%)
 Frame = -1

Query: 263  FD*SIANVASHFLNFLEEFPPGLRSSADRAESQHQREDEAQNTYEIHFTEXL 108
            +D S  N+ S  +NFL +F       A    SQ+QRED+ QNT +I  +E L
Sbjct: 1147 YDLSKYNLISSIINFLSKF-------ATENTSQYQREDDPQNTEDIVASEGL 1191


>UniRef50_Q0Q027 Cluster: Putative defense protein; n=1; Antheraea
           mylitta|Rep: Putative defense protein - Antheraea
           mylitta (Tasar silkworm)
          Length = 144

 Score = 33.1 bits (72), Expect = 9.2
 Identities = 21/79 (26%), Positives = 41/79 (51%)
 Frame = +3

Query: 219 KIEKVGRXVRDGLIKAGPAIAVIGQAKSLGK*TS*YSTKDAFSLKQYCK*L*ISSLNDLR 398
           ++E +G+ VRD +I AGPAI V+  +    +  +  +T D+  L Q  +   + +L+   
Sbjct: 55  ELEGIGQRVRDSIIIAGPAIDVLQMSHRSFRRQTNLTTNDSKVLLQIIRKCIVQTLHSSN 114

Query: 399 SYLNSIRHFYIYYVTLCYV 455
             + +I +    YV L ++
Sbjct: 115 YPIPNIYYTRTMYVCLVHI 133


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 465,139,391
Number of Sequences: 1657284
Number of extensions: 7707346
Number of successful extensions: 15576
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 15234
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15571
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 75833093035
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)