Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_L19
(904 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 81 1e-15
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 76 4e-14
At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 46 3e-05
At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 43 2e-04
At2g02930.1 68415.m00241 glutathione S-transferase, putative 40 0.002
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 39 0.005
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 39 0.005
At4g02520.1 68417.m00345 glutathione S-transferase, putative 38 0.007
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 36 0.037
At1g16840.2 68414.m02028 expressed protein 30 2.4
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 29 3.2
At1g04380.1 68414.m00428 2-oxoglutarate-dependent dioxygenase, p... 28 7.4
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 81.0 bits (191), Expect = 1e-15
Identities = 49/121 (40%), Positives = 65/121 (53%), Gaps = 4/121 (3%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVA----NESLRGGXLATQARVWQW 354
NK+ AFL P GKVP E+ +G V ESNAIA YV+ + SL G L A++ QW
Sbjct: 40 NKTPAFLKMNPIGKVPVLETPEGSVF--ESNAIARYVSRLNGDNSLNGSSLIEYAQIEQW 97
Query: 355 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 534
+S E+ + W P +G M ++ E A S L AL L+ HL + T+LV IT
Sbjct: 98 IDFSSLEIYASILRWFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSIT 157
Query: 535 L 537
L
Sbjct: 158 L 158
Score = 36.7 bits (81), Expect = 0.021
Identities = 25/77 (32%), Positives = 35/77 (45%)
Frame = +2
Query: 536 LADVIVFSTLLHAFQHVLXPSVRSSLINVQRWFLTVAXQPQVSAVVGSLTLCAXPPTYXP 715
LAD+I L F V+ S +V+R+F TV QP + V+G + P
Sbjct: 158 LADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIAS 217
Query: 716 KKYQELAGAXNKKEGKK 766
KK + A K+E KK
Sbjct: 218 KKAAQ--PAKPKEEPKK 232
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 75.8 bits (178), Expect = 4e-14
Identities = 47/121 (38%), Positives = 61/121 (50%), Gaps = 4/121 (3%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVANE----SLRGGXLATQARVWQW 354
NKS FL P GKVP E+ +G + ESNAIA YV+ + SL G L A + QW
Sbjct: 40 NKSPEFLKMNPIGKVPVLETPEGPIF--ESNAIARYVSRKNGDNSLNGSSLIEYAHIEQW 97
Query: 355 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 534
+S E+ W P +G F+ E A S L L+ L+ HL + TFLV +T
Sbjct: 98 IDFSSLEIDANMLKWFAPRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVT 157
Query: 535 L 537
L
Sbjct: 158 L 158
Score = 35.9 bits (79), Expect = 0.037
Identities = 24/81 (29%), Positives = 36/81 (44%)
Frame = +2
Query: 536 LADVIVFSTLLHAFQHVLXPSVRSSLINVQRWFLTVAXQPQVSAVVGSLTLCAXPPTYXP 715
LAD++ L F V+ S+ +V+R+F T+ QP+ V+G P
Sbjct: 158 LADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPT 217
Query: 716 KKYQELAGAXNKKEGKKIXSV 778
KK + A K+E KK V
Sbjct: 218 KKAPQ--PAKPKEEPKKAAPV 236
>At1g02930.1 68414.m00260 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 208
Score = 46.4 bits (105), Expect = 3e-05
Identities = 55/173 (31%), Positives = 74/173 (42%), Gaps = 14/173 (8%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVANE-SLRGGXLAT----QARVWQ 351
+K E F+ + P GKVPAFE D K+ ES AI Y+A+E S +G L + A +
Sbjct: 41 HKKEPFILRNPFGKVPAFEDGDFKIF--ESRAITQYIAHEFSDKGNNLLSTGKDMAIIAM 98
Query: 352 WASWSDSELLP--ASCAW--VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLV 519
E P + W V L M +K VE ++ L L V + L +L
Sbjct: 99 GIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLA 158
Query: 520 TERITLGRCH---CLQYTAACFPARARXERPFVADKRSALVPD--RRPXATSV 663
++ TL H +QY + ERP V SA V D RP A V
Sbjct: 159 SDHFTLVDLHTIPVIQYLLGTPTKKLFDERPHV----SAWVADITSRPSAQKV 207
>At1g02920.1 68414.m00259 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]; supported by cDNA GI:443697.
Length = 209
Score = 43.2 bits (97), Expect = 2e-04
Identities = 55/174 (31%), Positives = 74/174 (42%), Gaps = 15/174 (8%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGXLATQ-----ARVW 348
+K E F+ + P GKVPAFE DG L ES AI Y+A+ S +G L + A +
Sbjct: 41 HKKEPFIFRNPFGKVPAFE--DGDFKLFESRAITQYIAHFYSDKGNQLVSLGSKDIAGIA 98
Query: 349 QWASWSDSELLP--ASCAW--VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 516
E P + W V L M +K VE ++ L L V + L +L
Sbjct: 99 MGIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYL 158
Query: 517 VTERITLGRCH---CLQYTAACFPARARXERPFVADKRSALVPD--RRPXATSV 663
+++ TL H +QY + ERP V SA V D RP A V
Sbjct: 159 ASDKFTLVDLHTIPVIQYLLGTPTKKLFDERPHV----SAWVADITSRPSAKKV 208
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 39.9 bits (89), Expect = 0.002
Identities = 46/157 (29%), Positives = 68/157 (43%), Gaps = 16/157 (10%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVANE-SLRGGXL--ATQARVWQWA 357
+K E FL + P G+VPAFE DG + L ES AI Y+A+ +G L A + Q+A
Sbjct: 41 HKKEPFLSRNPFGQVPAFE--DGDLKLFESRAITQYIAHRYENQGTNLLPADSKNIAQYA 98
Query: 358 SWS--------DSELLPASCAW--VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTR 507
S + + + AW VF + + ++ V ++ L L V + L
Sbjct: 99 IMSIGIQVEAHQFDPVASKLAWEQVFKFNYGLNTDQAVVAEEEAKLAKVLDVYEARLKEF 158
Query: 508 TFLVTERITLGRCH---CLQYTAACFPARARXERPFV 609
+L E TL H +QY + ERP V
Sbjct: 159 KYLAGETFTLTDLHHIPVIQYLLGTPTKKLFTERPRV 195
>At1g02950.2 68414.m00263 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 245
Score = 38.7 bits (86), Expect = 0.005
Identities = 41/135 (30%), Positives = 58/135 (42%), Gaps = 12/135 (8%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGXL------ATQARV 345
+K+E FL P G+VP FE DG V L ES AI Y+A S RG L T A +
Sbjct: 64 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQLLNLRSHETMATL 121
Query: 346 WQWASWSDSELLP--ASCAW---VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRT 510
W + P + W + P G ++ ++ V+ ++ L L + + L
Sbjct: 122 TMWMEIEAHQFDPPASKLTWEQVIKPIYG-LETDQTIVKENEAILEKVLNIYEKRLEESR 180
Query: 511 FLVTERITLGRCHCL 555
FL TL H L
Sbjct: 181 FLACNSFTLVDLHHL 195
>At1g02950.1 68414.m00262 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 243
Score = 38.7 bits (86), Expect = 0.005
Identities = 41/135 (30%), Positives = 58/135 (42%), Gaps = 12/135 (8%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGXL------ATQARV 345
+K+E FL P G+VP FE DG V L ES AI Y+A S RG L T A +
Sbjct: 62 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQLLNLRSHETMATL 119
Query: 346 WQWASWSDSELLP--ASCAW---VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRT 510
W + P + W + P G ++ ++ V+ ++ L L + + L
Sbjct: 120 TMWMEIEAHQFDPPASKLTWEQVIKPIYG-LETDQTIVKENEAILEKVLNIYEKRLEESR 178
Query: 511 FLVTERITLGRCHCL 555
FL TL H L
Sbjct: 179 FLACNSFTLVDLHHL 193
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 38.3 bits (85), Expect = 0.007
Identities = 20/39 (51%), Positives = 26/39 (66%)
Frame = +1
Query: 187 NKSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVAN 303
+K E FL + P G+VPAFE DG + L ES AI Y+A+
Sbjct: 41 HKKEPFLSRNPFGQVPAFE--DGDLKLFESRAITQYIAH 77
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 35.9 bits (79), Expect = 0.037
Identities = 41/155 (26%), Positives = 64/155 (41%), Gaps = 15/155 (9%)
Frame = +1
Query: 190 KSEAFLXQFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGXLAT--------QAR 342
K +FL P G+VP F DG + LTES AI+ Y+A RG L R
Sbjct: 77 KKPSFLAINPFGQVPVF--LDGGLKLTESRAISEYIATVHKSRGTQLLNYKSYKTMGTQR 134
Query: 343 VWQWASWSDSELLPASCAW---VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTF 513
+W + + L ++ W + P G ++ + + V ++ L L + + L +F
Sbjct: 135 MWMAIESFEFDPLTSTLTWEQSIKPMYG-LKTDYKVVNETEAKLEKVLDIYEERLKNSSF 193
Query: 514 LVTERITLGRCHCL---QYTAACFPARARXERPFV 609
L + T+ + L QY R RP V
Sbjct: 194 LASNSFTMADLYHLPNIQYLMDTHTKRMFVNRPSV 228
>At1g16840.2 68414.m02028 expressed protein
Length = 161
Score = 29.9 bits (64), Expect = 2.4
Identities = 20/61 (32%), Positives = 26/61 (42%)
Frame = +2
Query: 308 VSAEEXWLPKPVSGSGHHGLTVNYCLLPVLGSSLTLVSCNSTNRMLNVQSLTYWPP*KYW 487
V W+P P SGS HG+ L+ L + LT STN S Y+ YW
Sbjct: 83 VPGASYWVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLSTNSSHGWPSSDYFLKGSYW 139
Query: 488 T 490
+
Sbjct: 140 S 140
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 29.5 bits (63), Expect = 3.2
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +2
Query: 413 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRES 532
L++ + N N +S + WPP K W D H + E+
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEA 234
>At1g04380.1 68414.m00428 2-oxoglutarate-dependent dioxygenase,
putative Strong similarity to Arabidopsis 2A6
(gb|X83096), tomato ethylene synthesis regulatory
protein E8 (SP|P10967)
Length = 345
Score = 28.3 bits (60), Expect = 7.4
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -2
Query: 363 P*CPLPDTGLGSQXSSAETFIGNVVSDGI 277
P CP PD LG+ S TFI ++ D I
Sbjct: 204 PPCPQPDQTLGTSKHSDNTFITILLQDNI 232
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,211,831
Number of Sequences: 28952
Number of extensions: 315931
Number of successful extensions: 788
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 769
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 786
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2129873112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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