Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_L18
(895 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 241 9e-65
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 233 3e-62
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 1e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 110 3e-25
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.3
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.3
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 241 bits (590), Expect = 9e-65
Identities = 106/158 (67%), Positives = 126/158 (79%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 506 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 619
+IR++AD C+GLQGFL+FH LL+ERL+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLA 158
Score = 107 bits (258), Expect = 2e-24
Identities = 46/63 (73%), Positives = 56/63 (88%)
Frame = +3
Query: 621 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDCAFMVDXEAIYDICRRNLD 800
++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH L+ +DC FMVD E+ YDICRRNLD
Sbjct: 159 MEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLD 218
Query: 801 IER 809
IER
Sbjct: 219 IER 221
Score = 40.3 bits (90), Expect = 4e-04
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = +2
Query: 809 PTYTNLNRLIGQIVSSITASLRSTAL*NV 895
P+Y NLNRLI Q+VSSITASLR NV
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNV 250
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 233 bits (569), Expect = 3e-62
Identities = 107/163 (65%), Positives = 128/163 (78%), Gaps = 5/163 (3%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 310
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 311 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 490
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 491 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 619
D VL+RIR++AD C+GLQGFL+FH LL+ERL+
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLN 162
Score = 113 bits (272), Expect = 3e-26
Identities = 50/66 (75%), Positives = 59/66 (89%)
Frame = +3
Query: 621 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDCAFMVDXEAIYDICRRNLD 800
++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH L++SDC FMVD EA YDICRRNLD
Sbjct: 163 MEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLD 222
Query: 801 IERQPT 818
IER PT
Sbjct: 223 IER-PT 227
Score = 42.3 bits (95), Expect = 9e-05
Identities = 21/29 (72%), Positives = 22/29 (75%)
Frame = +2
Query: 809 PTYTNLNRLIGQIVSSITASLRSTAL*NV 895
PTY NLNRLI Q+VSSITASLR NV
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNV 254
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (300), Expect = 1e-29
Identities = 61/157 (38%), Positives = 86/157 (54%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 506 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERL 616
+R+ A+ C LQGF + H LL+ ++
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKI 155
Score = 52.0 bits (119), Expect = 1e-07
Identities = 24/61 (39%), Positives = 36/61 (59%)
Frame = +3
Query: 624 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDCAFMVDXEAIYDICRRNLDI 803
+Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +D EA+ I L I
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217
Query: 804 E 806
+
Sbjct: 218 K 218
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 110 bits (264), Expect = 3e-25
Identities = 58/159 (36%), Positives = 90/159 (56%), Gaps = 2/159 (1%)
Frame = +2
Query: 149 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 328
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 329 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 502
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 503 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 619
D I + AD L+GF + H L+ERL+
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
Score = 34.3 bits (75), Expect = 0.024
Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = +3
Query: 627 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTXLEHSDCAFMVDXEAIYDICRRNLDI 803
Y KK ++++P Q VS VV+PYNS+L ++D ++D A+ I L
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220
Query: 804 ERQPT 818
+ PT
Sbjct: 221 Q-NPT 224
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,424,778
Number of Sequences: 5004
Number of extensions: 70643
Number of successful extensions: 201
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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