BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_L06 (1050 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 125 9e-30 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 125 9e-30 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 125 9e-30 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 68 3e-12 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 54 3e-08 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 1.1 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.1 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 1.9 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 28 2.5 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 28 2.5 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 5.8 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 7.7 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 125 bits (302), Expect = 9e-30 Identities = 61/83 (73%), Positives = 64/83 (77%) Frame = +2 Query: 122 MGKEXTHINHCPSSRHVDSGKSTTTGHLIYKCGGIDXRTIXXFXXEAHEMGXGSFKYAWV 301 MGKE HIN HVDSGKSTTTGHLIYKCGGID RTI F EA E+G GSFKYAWV Sbjct: 1 MGKEKGHINVVVIG-HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWV 59 Query: 302 LDXLXADRXRGIXIDIALWKFQT 370 LD L A+R RGI IDIALWKF+T Sbjct: 60 LDKLKAERERGITIDIALWKFET 82 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 125 bits (302), Expect = 9e-30 Identities = 61/83 (73%), Positives = 64/83 (77%) Frame = +2 Query: 122 MGKEXTHINHCPSSRHVDSGKSTTTGHLIYKCGGIDXRTIXXFXXEAHEMGXGSFKYAWV 301 MGKE HIN HVDSGKSTTTGHLIYKCGGID RTI F EA E+G GSFKYAWV Sbjct: 1 MGKEKGHINVVVIG-HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWV 59 Query: 302 LDXLXADRXRGIXIDIALWKFQT 370 LD L A+R RGI IDIALWKF+T Sbjct: 60 LDKLKAERERGITIDIALWKFET 82 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 125 bits (302), Expect = 9e-30 Identities = 61/83 (73%), Positives = 64/83 (77%) Frame = +2 Query: 122 MGKEXTHINHCPSSRHVDSGKSTTTGHLIYKCGGIDXRTIXXFXXEAHEMGXGSFKYAWV 301 MGKE HIN HVDSGKSTTTGHLIYKCGGID RTI F EA E+G GSFKYAWV Sbjct: 1 MGKEKGHINVVVIG-HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWV 59 Query: 302 LDXLXADRXRGIXIDIALWKFQT 370 LD L A+R RGI IDIALWKF+T Sbjct: 60 LDKLKAERERGITIDIALWKFET 82 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 67.7 bits (158), Expect = 3e-12 Identities = 30/74 (40%), Positives = 45/74 (60%) Frame = +2 Query: 149 HCPSSRHVDSGKSTTTGHLIYKCGGIDXRTIXXFXXEAHEMGXGSFKYAWVLDXLXADRX 328 H + HVDSGKST G ++++ G I+ R++ EA G GSF YAW+LD +R Sbjct: 179 HLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERA 238 Query: 329 RGIXIDIALWKFQT 370 RG+ +D+A F++ Sbjct: 239 RGVTMDVASTTFES 252 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 54.0 bits (124), Expect = 3e-08 Identities = 24/68 (35%), Positives = 39/68 (57%) Frame = +2 Query: 167 HVDSGKSTTTGHLIYKCGGIDXRTIXXFXXEAHEMGXGSFKYAWVLDXLXADRXRGIXID 346 HVD+GKST G++++ G +D RT+ EA E G S+ +W LD +R +G ++ Sbjct: 246 HVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVE 305 Query: 347 IALWKFQT 370 + F+T Sbjct: 306 VGRAYFET 313 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 29.1 bits (62), Expect = 1.1 Identities = 22/75 (29%), Positives = 33/75 (44%) Frame = +2 Query: 128 KEXTHINHCPSSRHVDSGKSTTTGHLIYKCGGIDXRTIXXFXXEAHEMGXGSFKYAWVLD 307 K I + S H+DSGK+T T ++Y G I + HE+ G +D Sbjct: 54 KRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMD 103 Query: 308 XLXADRXRGIXIDIA 352 + +R +GI I A Sbjct: 104 FMELEREKGITIQSA 118 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.1 bits (62), Expect = 1.1 Identities = 17/69 (24%), Positives = 21/69 (30%), Gaps = 1/69 (1%) Frame = +1 Query: 592 PGPPTTMXXHPXPRTSPXAXAPGPQSXEXTAXLXHLPP-PXXXPXTTPAXXTYPXPPIXP 768 P P P P +P P + A +PP P P PP+ Sbjct: 1143 PKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPK 1202 Query: 769 PXXGXPQXP 795 P G P P Sbjct: 1203 PSVGVPPVP 1211 Score = 28.3 bits (60), Expect = 1.9 Identities = 16/69 (23%), Positives = 25/69 (36%) Frame = +1 Query: 592 PGPPTTMXXHPXPRTSPXAXAPGPQSXEXTAXLXHLPPPXXXPXTTPAXXTYPXPPIXPP 771 P P P P++S A + P ++ + +P P P P PP+ P Sbjct: 1041 PPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAP---PVPAPSGIPPVPKP 1097 Query: 772 XXGXPQXPR 798 P P+ Sbjct: 1098 SVAAPPVPK 1106 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 28.3 bits (60), Expect = 1.9 Identities = 13/32 (40%), Positives = 13/32 (40%) Frame = +1 Query: 700 PPPXXXPXTTPAXXTYPXPPIXPPXXGXPQXP 795 PPP TPA P PP P G P P Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP 765 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 27.9 bits (59), Expect = 2.5 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = +2 Query: 122 MGKEXTHINHCPSSRHVDSGKSTTTGHLIYKCG 220 MGK +++ + HVD GKST T L+ K G Sbjct: 13 MGKP-SNVRNMSVIAHVDHGKSTLTDSLVQKAG 44 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 27.9 bits (59), Expect = 2.5 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = +2 Query: 122 MGKEXTHINHCPSSRHVDSGKSTTTGHLIYKCG 220 MGK +++ + HVD GKST T L+ K G Sbjct: 13 MGKP-SNVRNMSVIAHVDHGKSTLTDSLVQKAG 44 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.6 bits (56), Expect = 5.8 Identities = 15/52 (28%), Positives = 20/52 (38%) Frame = +1 Query: 640 PXAXAPGPQSXEXTAXLXHLPPPXXXPXTTPAXXTYPXPPIXPPXXGXPQXP 795 P A + P + T+ + LPPP T + PP PP G P Sbjct: 888 PGASSAQPAAMSRTSSVSTLPPPPPTASMTASAPAIASPP--PPKVGETYHP 937 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 7.7 Identities = 19/73 (26%), Positives = 24/73 (32%), Gaps = 4/73 (5%) Frame = +1 Query: 592 PGPPTTMXXHPXPRTSPXAXAPGPQSXEXTAXLXHLPPPXXXPXTTPAXXTYP--XPPIX 765 P PPT P ++P + P S + PP PA P PP Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSA 187 Query: 766 P--PXXGXPQXPR 798 P P P P+ Sbjct: 188 PSLPSAVPPMPPK 200 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,231,154 Number of Sequences: 5004 Number of extensions: 30465 Number of successful extensions: 91 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 59 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 86 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 551223336 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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