BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_K03
(902 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF513639-1|AAM53611.1| 195|Anopheles gambiae glutathione S-tran... 84 6e-18
L07880-1|AAA29358.1| 218|Anopheles gambiae glutathione S-transf... 77 6e-16
AF515526-1|AAM61893.1| 229|Anopheles gambiae glutathione S-tran... 31 0.063
Z81291-1|CAB03592.1| 209|Anopheles gambiae GSTD1-5 protein prot... 29 0.19
AF071160-3|AAC79993.1| 209|Anopheles gambiae glutathione S-tran... 29 0.19
AF515525-1|AAM61892.1| 235|Anopheles gambiae glutathione S-tran... 29 0.26
AY063776-1|AAL59658.1| 224|Anopheles gambiae glutathione S-tran... 26 1.8
AF316635-1|AAG45163.1| 224|Anopheles gambiae glutathione S-tran... 26 1.8
AF515521-1|AAM61888.1| 233|Anopheles gambiae glutathione S-tran... 25 3.1
AF117750-1|AAD38336.1| 380|Anopheles gambiae serine protease 18... 25 3.1
AY017417-1|AAG54081.1| 383|Anopheles gambiae arrestin protein. 24 5.5
AJ304409-1|CAC39103.2| 383|Anopheles gambiae arrestin protein. 24 5.5
AF071162-1|AAC79998.1| 216|Anopheles gambiae glutathione S-tran... 24 5.5
AF071160-2|AAC79994.1| 216|Anopheles gambiae glutathione S-tran... 24 5.5
M93690-1|AAA29364.1| 613|Anopheles gambiae ORF1 protein. 23 9.6
AY028786-1|AAK32960.1| 501|Anopheles gambiae cytochrome P450 pr... 23 9.6
>AF513639-1|AAM53611.1| 195|Anopheles gambiae glutathione
S-transferase S1-2 protein.
Length = 195
Score = 83.8 bits (198), Expect = 6e-18
Identities = 41/86 (47%), Positives = 56/86 (65%)
Frame = +2
Query: 254 GQMPVLVIDGKQYAQSTAICRYLGRKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYE 433
GQMPVL +DGK+ QS A+ RYL + GLAGA+D E ID V+ ++D R K A V YE
Sbjct: 41 GQMPVLEVDGKKVHQSVAMSRYLANQVGLAGADDWENLMIDTVVDTVNDFRLKIAVVSYE 100
Query: 434 ADEELKAKKHEDFSKNVYPXYVEEVE 511
D+E+K KK + V P Y+E+++
Sbjct: 101 PDDEIKEKKLVTLNNEVIPFYLEKLD 126
Score = 54.8 bits (126), Expect = 3e-09
Identities = 26/84 (30%), Positives = 46/84 (54%)
Frame = +3
Query: 450 KQKNTRTFRKTYTLXMLKKLNSIVEANKGHIAAGKLTWGDFVFTSMFDYLKTMLQIPDLE 629
K+K T L+KL+ I N G++A KL+W D FT++ DYL M + DL
Sbjct: 106 KEKKLVTLNNEVIPFYLEKLDDIARDNNGYLANSKLSWADIYFTAILDYLNYMTK-SDLV 164
Query: 630 VQYPAFKKVLQSVLTQPKVKAFLD 701
+P ++V+ +V + +++++D
Sbjct: 165 ANHPNLQRVVDNVTSIESIRSWID 188
Score = 43.2 bits (97), Expect = 1e-05
Identities = 19/39 (48%), Positives = 23/39 (58%)
Frame = +1
Query: 133 FACKALGEXGRMLLAYGGQDFEDHRVLSADWPDFKPKTP 249
F KALGE R LL+YG F+D R+ +WP KP P
Sbjct: 1 FNVKALGEPLRFLLSYGNLPFDDVRITREEWPALKPTMP 39
>L07880-1|AAA29358.1| 218|Anopheles gambiae glutathione
S-transferase protein.
Length = 218
Score = 77.4 bits (182), Expect = 6e-16
Identities = 37/86 (43%), Positives = 54/86 (62%)
Frame = +2
Query: 257 QMPVLVIDGKQYAQSTAICRYLGRKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYEA 436
QMPVL +DGK+ QS A+CRY+ ++ LAG N EA +ID V+ ++D R K A V YE
Sbjct: 65 QMPVLEVDGKRVHQSLAMCRYVAKQINLAGDNPLEALQIDAIVDTINDFRLKIAIVAYEP 124
Query: 437 DEELKAKKHEDFSKNVYPXYVEEVEL 514
D+ +K KK + V P Y+ ++ +
Sbjct: 125 DDMVKEKKMVTLNNEVIPFYLTKLNV 150
Score = 57.6 bits (133), Expect = 5e-10
Identities = 30/83 (36%), Positives = 42/83 (50%)
Frame = +3
Query: 450 KQKNTRTFRKTYTLXMLKKLNSIVEANKGHIAAGKLTWGDFVFTSMFDYLKTMLQIPDLE 629
K+K T L KLN I + N GH+ GK TW D F + DYL + + LE
Sbjct: 129 KEKKMVTLNNEVIPFYLTKLNVIAKENNGHLVLGKPTWADVYFAGILDYLNYLTKTNLLE 188
Query: 630 VQYPAFKKVLQSVLTQPKVKAFL 698
+P ++V+Q VL VKA++
Sbjct: 189 -NFPNLQEVVQKVLDNENVKAYI 210
Score = 51.2 bits (117), Expect = 4e-08
Identities = 23/49 (46%), Positives = 28/49 (57%)
Frame = +1
Query: 109 MPKVVYHYFACKALGEXGRMLLAYGGQDFEDHRVLSADWPDFKPKTPXR 255
MP +YF KALGE R LL+YG F+D R+ +WP KP P R
Sbjct: 16 MPDYKVYYFNVKALGEPLRFLLSYGNLPFDDVRITREEWPALKPTMPMR 64
>AF515526-1|AAM61893.1| 229|Anopheles gambiae glutathione
S-transferase protein.
Length = 229
Score = 30.7 bits (66), Expect = 0.063
Identities = 18/66 (27%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
Frame = +2
Query: 248 RFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAG----ANDEEAFEIDQNVEFLHDIRAKA 415
RF ++P + + A+S AI RYL R+Y + A+ +D+ +E+ H
Sbjct: 52 RFQKVPCITDSQIKLAESVAIFRYLCREYQVPDHWYPADSRRQALVDEYLEWQHHNTRAT 111
Query: 416 AAVYYE 433
A+Y++
Sbjct: 112 CAIYFQ 117
>Z81291-1|CAB03592.1| 209|Anopheles gambiae GSTD1-5 protein
protein.
Length = 209
Score = 29.1 bits (62), Expect = 0.19
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 260 MPVLVIDGKQYAQSTAICRYLGRKYG 337
+P LV +G +S AIC YL KYG
Sbjct: 52 IPTLVDNGFALWESRAICTYLAEKYG 77
>AF071160-3|AAC79993.1| 209|Anopheles gambiae glutathione
S-transferase protein.
Length = 209
Score = 29.1 bits (62), Expect = 0.19
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 260 MPVLVIDGKQYAQSTAICRYLGRKYG 337
+P LV +G +S AIC YL KYG
Sbjct: 52 IPTLVDNGFALWESRAICTYLAEKYG 77
>AF515525-1|AAM61892.1| 235|Anopheles gambiae glutathione
S-transferase protein.
Length = 235
Score = 28.7 bits (61), Expect = 0.26
Identities = 17/66 (25%), Positives = 36/66 (54%), Gaps = 5/66 (7%)
Frame = +2
Query: 248 RFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAG----ANDEEAFEIDQNVEFLH-DIRAK 412
R+G++P +V + A+S AI RYL R++ G ++ +D+ + + H ++RA
Sbjct: 53 RYGKVPCIVDGSFRLAESVAIYRYLCREFPTDGHWYPSDTVRQARVDEYLSWQHLNLRAD 112
Query: 413 AAAVYY 430
+ ++
Sbjct: 113 VSLYFF 118
>AY063776-1|AAL59658.1| 224|Anopheles gambiae glutathione
S-transferase E1 protein.
Length = 224
Score = 25.8 bits (54), Expect = 1.8
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +2
Query: 260 MPVLVIDGKQYAQSTAICRYLGRKYGLAGAND 355
+PVL +G ++S AI YL RKYG D
Sbjct: 55 IPVLDDNGTIISESHAIMIYLVRKYGQGEGKD 86
>AF316635-1|AAG45163.1| 224|Anopheles gambiae glutathione
S-transferase E1 protein.
Length = 224
Score = 25.8 bits (54), Expect = 1.8
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +2
Query: 260 MPVLVIDGKQYAQSTAICRYLGRKYGLAGAND 355
+PVL +G ++S AI YL RKYG D
Sbjct: 55 IPVLDDNGTIISESHAIMIYLVRKYGQGEGKD 86
>AF515521-1|AAM61888.1| 233|Anopheles gambiae glutathione
S-transferase u1 protein.
Length = 233
Score = 25.0 bits (52), Expect = 3.1
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +2
Query: 257 QMPVLVIDGKQYAQSTAICRYLGRKY 334
++PVL DG ++S AI +YL KY
Sbjct: 51 EIPVLDDDGFFLSESNAILQYLCEKY 76
>AF117750-1|AAD38336.1| 380|Anopheles gambiae serine protease 18D
protein.
Length = 380
Score = 25.0 bits (52), Expect = 3.1
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +2
Query: 107 KCLRLCTITSLARLSGXAAVCCSPTEARTSKTTACSLLTGLTSSQR 244
KC R IT + + A VCC ++ S + S+ T L S R
Sbjct: 40 KCKRGNRITVCSYSATEAIVCCPQSQQLDSPPSGFSIPTPLNSQSR 85
>AY017417-1|AAG54081.1| 383|Anopheles gambiae arrestin protein.
Length = 383
Score = 24.2 bits (50), Expect = 5.5
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +1
Query: 118 VVYHYFACKALGEXGRMLLAYGGQDFEDH 204
+VY++ K G++ L G +DF DH
Sbjct: 1 MVYNFKVFKKCAPNGKVTLYMGKRDFVDH 29
>AJ304409-1|CAC39103.2| 383|Anopheles gambiae arrestin protein.
Length = 383
Score = 24.2 bits (50), Expect = 5.5
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +1
Query: 118 VVYHYFACKALGEXGRMLLAYGGQDFEDH 204
+VY++ K G++ L G +DF DH
Sbjct: 1 MVYNFKVFKKCAPNGKVTLYMGKRDFVDH 29
>AF071162-1|AAC79998.1| 216|Anopheles gambiae glutathione
S-transferase D1-4 protein.
Length = 216
Score = 24.2 bits (50), Expect = 5.5
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = +2
Query: 260 MPVLVIDGKQYAQSTAICRYLGRKYGLAGAND 355
+P LV G +S AI YL KYG ND
Sbjct: 52 VPTLVDSGFALWESRAIMCYLVEKYGKPCNND 83
>AF071160-2|AAC79994.1| 216|Anopheles gambiae glutathione
S-transferase protein.
Length = 216
Score = 24.2 bits (50), Expect = 5.5
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = +2
Query: 260 MPVLVIDGKQYAQSTAICRYLGRKYGLAGAND 355
+P LV G +S AI YL KYG ND
Sbjct: 52 VPTLVDSGFALWESRAIMCYLVEKYGKPCNND 83
>M93690-1|AAA29364.1| 613|Anopheles gambiae ORF1 protein.
Length = 613
Score = 23.4 bits (48), Expect = 9.6
Identities = 8/24 (33%), Positives = 16/24 (66%)
Frame = +2
Query: 209 CSLLTGLTSSQRPRFGQMPVLVID 280
C++L G + P G++P++V+D
Sbjct: 80 CTILAGPSGLAVPPMGKVPLVVLD 103
>AY028786-1|AAK32960.1| 501|Anopheles gambiae cytochrome P450
protein.
Length = 501
Score = 23.4 bits (48), Expect = 9.6
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +3
Query: 624 LEVQYPAFKKVLQSVLTQPKVKAF 695
L Q+P + L+ LTQP V F
Sbjct: 221 LSAQFPKIARTLRVKLTQPDVAEF 244
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 703,584
Number of Sequences: 2352
Number of extensions: 13035
Number of successful extensions: 43
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 43
length of database: 563,979
effective HSP length: 64
effective length of database: 413,451
effective search space used: 97574436
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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