Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_J20
(907 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 121 1e-28
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 81 2e-16
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 80 5e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 66 5e-12
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.39
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 29 1.2
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 2.8
SPAC1610.03c |crp79|meu5|poly|Schizosaccharomyces pombe|chr 1|||... 27 4.8
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 4.8
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.8
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 27 4.8
SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc... 26 6.4
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 26 6.4
SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces ... 26 8.5
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 121 bits (292), Expect = 1e-28
Identities = 51/66 (77%), Positives = 60/66 (90%)
Frame = +2
Query: 323 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 502
DLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+
Sbjct: 67 DLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEA 126
Query: 503 CDCLQG 520
CD LQG
Sbjct: 127 CDALQG 132
Score = 118 bits (284), Expect = 1e-27
Identities = 57/97 (58%), Positives = 67/97 (69%)
Frame = +3
Query: 510 AYRGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLS 689
A +GFQ KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188
Query: 690 VHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDL 800
+HQLVEN+DET+CIDNEAL I TLK+ +P+Y DL
Sbjct: 189 MHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDL 225
Score = 109 bits (262), Expect = 5e-25
Identities = 46/68 (67%), Positives = 55/68 (80%)
Frame = +1
Query: 124 MRXXVHLQAGQCGNQLGAKFWXLISDEHGIDPTGAYHGXSDLQLERINVYYNEASGGKYV 303
MR VH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 304 PRAILXRL 327
PRA+L L
Sbjct: 61 PRAVLVDL 68
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 81.0 bits (191), Expect = 2e-16
Identities = 32/66 (48%), Positives = 48/66 (72%)
Frame = +2
Query: 323 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 502
DLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132
Query: 503 CDCLQG 520
C LQG
Sbjct: 133 CSGLQG 138
Score = 76.6 bits (180), Expect = 5e-15
Identities = 33/72 (45%), Positives = 49/72 (68%)
Frame = +3
Query: 585 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 764
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 765 TLKLSTPTYGDL 800
L + PTY +L
Sbjct: 220 NLDIERPTYENL 231
Score = 43.2 bits (97), Expect = 5e-05
Identities = 23/70 (32%), Positives = 33/70 (47%), Gaps = 6/70 (8%)
Frame = +1
Query: 124 MRXXVHLQAGQCGNQLGAKFWXLISDEHGIDPTG------AYHGXSDLQLERINVYYNEA 285
MR + + GQ G Q+G W L EHGI P G H + + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 286 SGGKYVPRAI 315
GK+VPR+I
Sbjct: 61 GQGKFVPRSI 70
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 79.8 bits (188), Expect = 5e-16
Identities = 32/66 (48%), Positives = 46/66 (69%)
Frame = +2
Query: 323 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 502
DLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128
Query: 503 CDCLQG 520
C LQG
Sbjct: 129 CSGLQG 134
Score = 70.1 bits (164), Expect = 4e-13
Identities = 30/72 (41%), Positives = 47/72 (65%)
Frame = +3
Query: 585 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 764
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 765 TLKLSTPTYGDL 800
L + P+Y +L
Sbjct: 216 NLDIERPSYENL 227
Score = 48.4 bits (110), Expect = 1e-06
Identities = 24/66 (36%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +1
Query: 124 MRXXVHLQAGQCGNQLGAKFWXLISDEHGIDPTGAYHGXSDLQLE--RINVYYNEASGGK 297
MR + + GQ G Q+G W L EHGI P G + + Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 298 YVPRAI 315
YVPR+I
Sbjct: 61 YVPRSI 66
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 66.5 bits (155), Expect = 5e-12
Identities = 29/70 (41%), Positives = 44/70 (62%)
Frame = +1
Query: 127 RXXVHLQAGQCGNQLGAKFWXLISDEHGIDPTGAYHGXSDLQLERINVYYNEASGGKYVP 306
R + LQAGQCGNQ+G++FW + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 307 RAILXRLGAR 336
RAIL L R
Sbjct: 63 RAILIDLEPR 72
Score = 60.1 bits (139), Expect = 4e-10
Identities = 29/70 (41%), Positives = 41/70 (58%), Gaps = 1/70 (1%)
Frame = +3
Query: 585 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICF 761
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 762 RTLKLSTPTY 791
L PT+
Sbjct: 216 DRLHTQNPTF 225
Score = 56.4 bits (130), Expect = 5e-09
Identities = 22/68 (32%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
Frame = +2
Query: 323 DLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKES 496
DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E+
Sbjct: 68 DLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREA 126
Query: 497 ESCDCLQG 520
+ D L+G
Sbjct: 127 DGSDSLEG 134
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.3 bits (65), Expect = 0.39
Identities = 25/82 (30%), Positives = 34/82 (41%)
Frame = +1
Query: 511 PTGASNLHIPSVAAPGPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQ 690
PTG S+ + P + SS+ + T T S T T SP P T + T +
Sbjct: 180 PTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSS-TDTNSSPLPTTSTSCTTSTSIPTG 238
Query: 691 FTS*LKTQTKPTASTTRLYTTS 756
+S L T PT T +TS
Sbjct: 239 GSSSLSTPITPTVPPTSTSSTS 260
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 28.7 bits (61), Expect = 1.2
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 428 AKGHYTEGAELVDSVLDVVRKESESCDCLQGLP 526
A+GH G ELV + D +RK+SE+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/53 (30%), Positives = 29/53 (54%)
Frame = +3
Query: 351 SARDLSDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAI 509
S+ S S++ ++S S S P+T+ +T LSS + SS S+ + + +I
Sbjct: 220 SSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSI 272
>SPAC1610.03c |crp79|meu5|poly|Schizosaccharomyces pombe|chr
1|||Manual
Length = 710
Score = 26.6 bits (56), Expect = 4.8
Identities = 21/102 (20%), Positives = 39/102 (38%)
Frame = -3
Query: 464 QRAQHPLCSVPWPSCCRRRTVRKRSCPDGRSVRKVPSGQSPWCRAPSRXRMARGTYLPPE 285
Q A L +VP+ +++ ++ +S+ P+G+S W +
Sbjct: 257 QNAIEKLNNVPYKGSILEVSIKNKASSSVKSIPTTPTGESLWPFPSENANKTQINIENAT 316
Query: 284 ASL*YTLMRSNCKSEXPW*APVGSMPCSSEMSXQNLAPSWLP 159
S +M S K + W GS+ + + QN +W P
Sbjct: 317 CSKMTWIMGSPTKEKSQW----GSVSTTGVSNQQNHPAAWNP 354
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.6 bits (56), Expect = 4.8
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = +1
Query: 511 PTGASNLHIPSVAAPGPVWAPSS 579
P+GA + PSVAAP PV APSS
Sbjct: 1155 PSGAPPVPKPSVAAP-PVPAPSS 1176
Score = 25.8 bits (54), Expect = 8.5
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +1
Query: 508 LPTGASNLHIPSVAAPGPVWAPSSS 582
+P+GA + PSVAAP PV APS +
Sbjct: 1135 VPSGAPPVPKPSVAAP-PVPAPSGA 1158
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.8
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 359 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 460
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 26.6 bits (56), Expect = 4.8
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +3
Query: 366 SDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SA 488
S SS T+ S + + T P ++ V+SS++ SSM S+
Sbjct: 342 SSSSSASATATSSAESSIATSPITSSSNVVSSISTSSMDSS 382
>SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein
4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 636
Score = 26.2 bits (55), Expect = 6.4
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +1
Query: 481 SPQRIRILRLPTGASNLHIPSVAAPGPVWAPSSSQRSVKSTPTES 615
+P + ++ P A+N+H+ SVAA PS+ V +T T S
Sbjct: 160 TPHKAGVVSNPA-AANVHVLSVAASPNPSTPSNGPAPVSTTATPS 203
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 26.2 bits (55), Expect = 6.4
Identities = 24/72 (33%), Positives = 31/72 (43%)
Frame = -2
Query: 417 PAPDCPKTKLSGRKICPKGPERTESMVPGSKSXEDGAGHVLAAGGFIVVYIDALQLQVRX 238
P+ PK L R I P GPE + + GS S A ++ +G + YID VR
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHSL--WAHYLWNSGIELANYIDKNPDTVRA 77
Query: 237 PMVGTGGVDAVL 202
V G A L
Sbjct: 78 KKVLELGAGAGL 89
>SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 886
Score = 25.8 bits (54), Expect = 8.5
Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +3
Query: 306 PRHPRSTWSPAPWTLSARDLSDRSSVRTTSFS-DSPAPATTGPRDTTQ 446
P+H R +PAP L + VRTT+ + P P TT T Q
Sbjct: 293 PQHRRFQSAPAPIPRPVSTLIPLTQVRTTASNVIKPRPQTTERPSTAQ 340
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,182,735
Number of Sequences: 5004
Number of extensions: 63963
Number of successful extensions: 236
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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