BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_J18 (904 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 177 1e-45 SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 158 1e-39 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 108 1e-24 SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 104 2e-23 SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 90 3e-19 SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 49 1e-06 SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 27 3.6 SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 26 6.4 SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharom... 26 6.4 >SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 177 bits (432), Expect = 1e-45 Identities = 85/182 (46%), Positives = 113/182 (62%) Frame = +1 Query: 262 GLFINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMD 441 GLFINN+ V S G K +P+ ++I EV F+ +PWR Sbjct: 24 GLFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTDAPWRKFS 83 Query: 442 ASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIH 621 +++RG +++LAD IE++ YLAS+ETLDNGK + GD+ A+ RYY GWADK + Sbjct: 84 SAQRGRCLSRLADCIEQNLEYLASIETLDNGKSITLAR-GDVQAAADCFRYYGGWADKDY 142 Query: 622 GNVLPADGKYFAYTRHEPVGVCGQIIPWNFPILMAAWKLGPALATGCTVVMXPAXXTPLT 801 G + D K FAYTRHEP+GVCGQIIPWNFP LM AWK+ PA+A G T+++ A TPL+ Sbjct: 143 GQTIETDIKRFAYTRHEPIGVCGQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELTPLS 202 Query: 802 XL 807 L Sbjct: 203 AL 204 >SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 158 bits (383), Expect = 1e-39 Identities = 79/194 (40%), Positives = 115/194 (59%) Frame = +1 Query: 262 GLFINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMD 441 GL+IN EW KS++ T++T +P+ +VIA+V FK W+ + Sbjct: 22 GLYINGEWHKSAE--TWETVDPSIEEVIAKVYLAGEKEIDYAVKSAKEAFKT---WKKVP 76 Query: 442 ASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIH 621 SE+G L+ KLA+L E+ LA++E +D+GKP + GD+ +I LRY AGWADKI+ Sbjct: 77 GSEKGELLMKLAELTEKHADTLAAIEAMDSGKPLVSNARGDVDGTIALLRYCAGWADKIY 136 Query: 622 GNVLPADGKYFAYTRHEPVGVCGQIIPWNFPILMAAWKLGPALATGCTVVMXPAXXTPLT 801 G V+P + AY + P+GVCGQI+PWN+P+ MA WK+ PALA G +++ A TPL+ Sbjct: 137 GQVIPTGPEKLAYAKRTPIGVCGQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAETTPLS 196 Query: 802 XLXHRSTXXGXRFP 843 L + FP Sbjct: 197 LLYFATLVEEAGFP 210 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 108 bits (259), Expect = 1e-24 Identities = 65/181 (35%), Positives = 95/181 (52%), Gaps = 1/181 (0%) Frame = +1 Query: 268 FINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDAS 447 +I +WV ++ GKTF ENP + +A V F +R D Sbjct: 27 YIGGKWVTAASGKTFDVENPGLNETLAPVTDMSVEETRKAIKVAHEAFL---SYRNSDIK 83 Query: 448 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN 627 ER A++ + DLI + LA++ TL+NGK D+ G++ + K + ++AG A +I G+ Sbjct: 84 ERYAILRRWYDLIMENADDLATMMTLENGKALGDAK-GEVVYAAKFIDWFAGEALRISGD 142 Query: 628 V-LPADGKYFAYTRHEPVGVCGQIIPWNFPILMAAWKLGPALATGCTVVMXPAXXTPLTX 804 + ++ + T +PVGV G I PWNFP M K+G ALA GCTVV+ PA TP T Sbjct: 143 SSMSSNPQNRIITIKQPVGVVGIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETPFTA 202 Query: 805 L 807 L Sbjct: 203 L 203 >SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 493 Score = 104 bits (250), Expect = 2e-23 Identities = 59/182 (32%), Positives = 90/182 (49%), Gaps = 2/182 (1%) Frame = +1 Query: 268 FINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDAS 447 F+ +W+ S + KTF+ +NPA G++I +V FK ++ Sbjct: 21 FVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKT---YKNFTHV 77 Query: 448 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN 627 +R L+ + A+LI ++ L + TL+NGKP + ++ L++YA A + G+ Sbjct: 78 QRSQLLERWAELIMENKDDLVKMLTLENGKPLSQAEM-EVTTCSGYLKWYAAEAVRTFGD 136 Query: 628 VLPAD--GKYFAYTRHEPVGVCGQIIPWNFPILMAAWKLGPALATGCTVVMXPAXXTPLT 801 V P+ + F + +PVGV I PWNFP M A K G ALA GCT + PA TP Sbjct: 137 VAPSSLQSQNFLISIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTPYV 196 Query: 802 XL 807 L Sbjct: 197 CL 198 >SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 500 Score = 90.2 bits (214), Expect = 3e-19 Identities = 57/186 (30%), Positives = 90/186 (48%), Gaps = 5/186 (2%) Frame = +1 Query: 265 LFINNEWVKSSD--GKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTM 438 LFI+ ++V + K NPA ++I + F+ G W Sbjct: 23 LFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTFRSGI-WAKW 81 Query: 439 DASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKI 618 +RG ++ K+A ++ R LA ++T++ GKP + F D+ + YYA A+ Sbjct: 82 PGKQRGLVLRKIAKMMREKRELLAGIDTINCGKPTPYALF-DIDSCADMFEYYAEVAETD 140 Query: 619 HGNV---LPADGKYFAYTRHEPVGVCGQIIPWNFPILMAAWKLGPALATGCTVVMXPAXX 789 + V LP + + A+ + P GV G I PWNFP+ MA WKL PA+A+G VV+ P+ Sbjct: 141 NPTVKVPLPNNPGFCAFEKRFPRGVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSEL 200 Query: 790 TPLTXL 807 P + L Sbjct: 201 APWSCL 206 >SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 48.8 bits (111), Expect = 1e-06 Identities = 29/126 (23%), Positives = 57/126 (45%), Gaps = 4/126 (3%) Frame = +1 Query: 427 WRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRY-YAG 603 W++ +ER + L + I R++ A + D GK D+ FG++ +++ + + A Sbjct: 41 WKSTSFAERRNFLKALKENIIRNQDKYAEIACKDTGKTLVDAAFGEILVTLEKINWTLAN 100 Query: 604 WADKIHGNVLPAD---GKYFAYTRHEPVGVCGQIIPWNFPILMAAWKLGPALATGCTVVM 774 + P Y ++EP+GV ++ WN+P+ A + AL G +V+ Sbjct: 101 GEQSLRPTKRPNSLLTSYKGGYVKYEPLGVIAALVSWNYPLHNALGPIISALFAGNAIVV 160 Query: 775 XPAXXT 792 + T Sbjct: 161 KGSELT 166 >SPAC139.03 |||transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 27.1 bits (57), Expect = 3.6 Identities = 14/44 (31%), Positives = 20/44 (45%), Gaps = 1/44 (2%) Frame = +3 Query: 600 RVGRQDPRECPA-RRRKILCLHSS*ASGCVRSDNTMELPYIDGG 728 + R+ C A RR+K+ C H S C++ YID G Sbjct: 14 QTSRRAIHSCLACRRKKLKCDHGRPCSNCLKRSTIQSCIYIDPG 57 >SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1692 Score = 26.2 bits (55), Expect = 6.4 Identities = 14/46 (30%), Positives = 21/46 (45%), Gaps = 1/46 (2%) Frame = +3 Query: 159 VAERLQIGSGAKSEFCNSCFSGTGSPNKT-GNFIHWSLHKQ*VGEV 293 +A + + + CN+ G NK GN IH+ L K G+V Sbjct: 1054 LAHEINAADSSSEQICNALRRGFLRLNKKLGNVIHYDLRKSSEGDV 1099 >SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 26.2 bits (55), Expect = 6.4 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = -1 Query: 604 TRRSTVGFLLKHTDHRNTSP 545 +RRST GFL KH+ ++ SP Sbjct: 19 SRRSTKGFLQKHSSLKDGSP 38 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,602,859 Number of Sequences: 5004 Number of extensions: 73637 Number of successful extensions: 182 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 173 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 456499320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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