BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_J17 (898 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 163 4e-41 SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 159 5e-40 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 29 0.68 SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 28 2.1 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 26 8.4 >SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 163 bits (395), Expect = 4e-41 Identities = 75/108 (69%), Positives = 89/108 (82%) Frame = +1 Query: 91 APKMVAAKKQKKXIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRK 270 AP +KK KK ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLRK Sbjct: 8 APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRK 67 Query: 271 SEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 414 SE+EYYA+L++ VHHYSGNNI+LGTACGK +RV LA+ D GDSDI+ Sbjct: 68 SELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDIL 115 >SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Schizosaccharomyces pombe|chr 1|||Manual Length = 109 Score = 159 bits (386), Expect = 5e-40 Identities = 73/104 (70%), Positives = 86/104 (82%) Frame = +1 Query: 103 VAAKKQKKXIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIE 282 V KK KK ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E Sbjct: 4 VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63 Query: 283 YYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 414 YYA+L+K VHHY+G NI+LGTACGK +RV LAITD GDSDI+ Sbjct: 64 YYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDIL 107 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 29.5 bits (63), Expect = 0.68 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = +1 Query: 115 KQKKXIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 240 ++KK I+ ++L SGKYC G T+ L++G + ++ Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318 >SPAC977.14c |||aldo/keto reductase, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 351 Score = 27.9 bits (59), Expect = 2.1 Identities = 15/45 (33%), Positives = 26/45 (57%) Frame = +1 Query: 223 KAKLVIIAKNAPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 357 ++ +VI++K P+RK I+ + L+ GVH ++ EL CG Sbjct: 87 RSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFL--DSPELANQCG 129 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 25.8 bits (54), Expect = 8.4 Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 1/57 (1%) Frame = +1 Query: 97 KMVAAKK-QKKXIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 264 K +A KK KK ++++ K G K+ +K +R+G+ LVI+A + P+ Sbjct: 30 KPLAPKKLNKKMMKTVKK----ASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPM 82 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,827,913 Number of Sequences: 5004 Number of extensions: 47457 Number of successful extensions: 106 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 106 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 452494940 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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