BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_J11 (902 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.30 SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.68 SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 29 0.90 SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 29 1.2 SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 2.1 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 27 4.8 SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 26 6.4 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 8.4 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 26 8.4 SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 26 8.4 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 30.7 bits (66), Expect = 0.30 Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%) Frame = +1 Query: 493 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 669 VTP P S S PP +T T S P +++ S T + T +ST + P P Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVP 360 Query: 670 SNTTGT 687 +T + Sbjct: 361 PTSTSS 366 Score = 30.7 bits (66), Expect = 0.30 Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%) Frame = +1 Query: 493 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 669 VTP P S S PP +T T S P +++ S T + T +ST + P P Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVP 414 Query: 670 SNTTGT 687 ++ T Sbjct: 415 PTSSST 420 Score = 29.9 bits (64), Expect = 0.52 Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Frame = +1 Query: 499 PKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSN 675 P T + S P P T+ TS S PP NST + +T V + SST P P++ Sbjct: 264 PPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNST-TPVTPTVPPTSTSSTSTPP-PPAS 321 Query: 676 TTGT 687 T+ T Sbjct: 322 TSST 325 Score = 29.5 bits (63), Expect = 0.68 Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%) Frame = +1 Query: 493 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 669 +TP P S S+P P T+ + S P T ST + + + S+T + P P Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306 Query: 670 SNTTGT 687 +T + Sbjct: 307 PTSTSS 312 Score = 29.1 bits (62), Expect = 0.90 Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%) Frame = +1 Query: 493 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 669 +TP P S S+P P T+ + S P T ST S T + SS++ P+TP Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249 Query: 670 S 672 + Sbjct: 250 T 250 Score = 28.3 bits (60), Expect = 1.6 Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 1/69 (1%) Frame = +1 Query: 484 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP 660 L++ TP T P S S W TT T S T+ST +T T ++ + Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657 Query: 661 LTPSNTTGT 687 T S++T T Sbjct: 658 STSSSSTAT 666 >SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 29.5 bits (63), Expect = 0.68 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = -1 Query: 575 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 474 DLE N +V Q+ G G+FL G FG + FV+ Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444 >SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schizosaccharomyces pombe|chr 3|||Manual Length = 215 Score = 29.1 bits (62), Expect = 0.90 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = +3 Query: 219 ETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKE 362 ET K S K +K + + +++++ K NT++ A Q W+K KE Sbjct: 128 ETPKKKHSLIRKRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKE 175 >SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 28.7 bits (61), Expect = 1.2 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = -1 Query: 707 FVHGGLKVPVVFEGVSGAITVDDTVIT 627 F H + V G+ GAIT+DD++IT Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIIT 133 >SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1237 Score = 27.9 bits (59), Expect = 2.1 Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = +3 Query: 186 QLYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 302 Q+++++V+ Y A+ EYL+EK I + K ++E Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 26.6 bits (56), Expect = 4.8 Identities = 23/89 (25%), Positives = 41/89 (46%) Frame = -2 Query: 289 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 110 F S ++ F R+S + S TT+ + S SVL ++ A+E S TA ++ Sbjct: 3567 FVPSSVSRSFSYSRFSSGSLDSSSVFNSTTVSTASGISQGSVLSSTRAIE-SESTASHRS 3625 Query: 109 NKVSLILAQWLSLKASQQTTQNLKGIPYS 23 + +S + + LS A ++ + YS Sbjct: 3626 SVLSELSSYDLSTSAFSSSSSEPSTLDYS 3654 >SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 892 Score = 26.2 bits (55), Expect = 6.4 Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 6/51 (11%) Frame = +3 Query: 627 SDDRIIYGDSTADTFKHHWYLEP----SMYESDVMFFVYNR--EYNSVMTL 761 SDDR+ GD+ + +Y++ +MYE ++ Y YN V+ L Sbjct: 84 SDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLAL 134 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 25.8 bits (54), Expect = 8.4 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +3 Query: 423 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 515 L+ K+D A K+ DQ H K+ +DK S Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 25.8 bits (54), Expect = 8.4 Identities = 34/130 (26%), Positives = 48/130 (36%), Gaps = 4/130 (3%) Frame = +3 Query: 453 KLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRV--YFKIMSTEDKQYLKLDNTKGS 626 K ID+ I +G K S+ F P E +R+ K++ ED LD K Sbjct: 219 KDIDENEEVTINYGSEKGSAEFLFSYGFLPEPEGDRITNVMKLLIPEDSND-SLDLAKRR 277 Query: 627 SDDRIIYGDSTADTFKHHWYLEPSMYESDVMFFVYNRE--YNSVMTLDEDMAANEXREAL 800 S + +D+ W+ P ++ F V N E N M DE A E Sbjct: 278 SCKTPPMIEFVSDSSGELWWHAPFLF-----FSVLNVEDFTNFKMVCDESKAQTVDWEFE 332 Query: 801 GHSGXVXVIP 830 G V +P Sbjct: 333 GQKCSVEDLP 342 >SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 417 Score = 25.8 bits (54), Expect = 8.4 Identities = 16/71 (22%), Positives = 31/71 (43%) Frame = -2 Query: 322 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVAL 143 M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++ Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57 Query: 142 EASAHTARTKA 110 +A A T A Sbjct: 58 NGAAKEAATAA 68 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,562,828 Number of Sequences: 5004 Number of extensions: 74660 Number of successful extensions: 303 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 271 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 301 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 456499320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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