BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_J11 (902 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 1.0 At1g62250.2 68414.m07023 expressed protein 30 1.8 At1g62250.1 68414.m07022 expressed protein 30 1.8 At5g09400.1 68418.m01089 potassium transporter family protein si... 29 4.2 At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 29 4.2 At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 5.6 At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 5.6 At1g64150.1 68414.m07267 expressed protein contains Pfam profile... 29 5.6 At1g47310.1 68414.m05238 expressed protein 28 7.4 At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 28 9.7 At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro... 28 9.7 At3g04160.1 68416.m00440 expressed protein ; expression supporte... 28 9.7 At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain... 28 9.7 >At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein / VPS11 family protein similar to Vacuolar protein sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo sapiens]; similar to Vacuolar biogenesis protein END1 (PEP5 protein) (Vacuolar protein sorting 11) (Swiss-Prot:P12868) [Saccharomyces cerevisiae] Length = 932 Score = 31.1 bits (67), Expect = 1.0 Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%) Frame = +3 Query: 207 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 380 +G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557 Query: 381 --PIQFRVIFTEQTVKLINKRDHHA 449 P+ F +F + L++ + +A Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582 >At1g62250.2 68414.m07023 expressed protein Length = 223 Score = 30.3 bits (65), Expect = 1.8 Identities = 12/43 (27%), Positives = 23/43 (53%) Frame = +1 Query: 103 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 231 LC P + +RW TP + E+++ WR C +++ R ++ Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222 >At1g62250.1 68414.m07022 expressed protein Length = 267 Score = 30.3 bits (65), Expect = 1.8 Identities = 12/43 (27%), Positives = 23/43 (53%) Frame = +1 Query: 103 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 231 LC P + +RW TP + E+++ WR C +++ R ++ Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222 >At5g09400.1 68418.m01089 potassium transporter family protein similar to K+ transporter HAK5 [Arabidopsis thaliana] GI:7108597; contains Pfam profile PF02705: K+ potassium transporter; KUP/HAK/KT Transporter family member, PMID:11500563; Note: possible sequencing error causes a frameshift in the 4th exon|15810448|gb|AY056263 Length = 858 Score = 29.1 bits (62), Expect = 4.2 Identities = 19/73 (26%), Positives = 35/73 (47%) Frame = -2 Query: 289 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 110 F +S+ S F+ + ++A ++ MTT +SC S+ LG L+ HT+R Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468 Query: 109 NKVSLILAQWLSL 71 ++ + + W L Sbjct: 469 GQIYIPVLNWFLL 481 >At1g77240.1 68414.m08996 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501 Length = 545 Score = 29.1 bits (62), Expect = 4.2 Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = +3 Query: 366 VKSYFPIQFRVIFTEQTVKLINKR-DHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTP 542 V S + +QF V + + IN R D HAL ++ + + +K+ F D + + F P Sbjct: 76 VPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLP 135 Query: 543 VLENNRV 563 E R+ Sbjct: 136 KDERPRL 142 >At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (ATHIM) identical to SP|P34881 DNA (cytosine-5)-methyltransferase AthI (EC 2.1.1.37) {Arabidopsis thaliana} Length = 1534 Score = 28.7 bits (61), Expect = 5.6 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = -3 Query: 351 PLSITGRRSPWCSSCRFRSDASR 283 PLS GR S +C+SC+ R D + Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891 >At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19) Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis thaliana]; similar to cytochrome P450LXXIA1, Persea americana, M32885 Length = 490 Score = 28.7 bits (61), Expect = 5.6 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = -1 Query: 449 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 357 G +P + +D + G+DH + + RFD+FL Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251 >At1g64150.1 68414.m07267 expressed protein contains Pfam profile PF01169: Uncharacterized protein family UPF0016 Length = 370 Score = 28.7 bits (61), Expect = 5.6 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = -1 Query: 668 GVSGAITVDDTVITRTFRVIELQVLFVLGGHDLEVNSV 555 G G +T+ V+ RTF ++ + F GG DL ++ + Sbjct: 197 GALGIMTIISVVLGRTFHYVDEVLPFRFGGTDLPIDDI 234 >At1g47310.1 68414.m05238 expressed protein Length = 395 Score = 28.3 bits (60), Expect = 7.4 Identities = 19/67 (28%), Positives = 35/67 (52%) Frame = +3 Query: 405 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 584 T QTV + + A ++ Q + + G ++ SKKV+W+ P +E RV+F++ + Sbjct: 286 TRQTVSSVTAK-LKASGMVRFQLEIERSIGKNESVISKKVAWRTKPKIE--RVWFEVTAK 342 Query: 585 EDKQYLK 605 + LK Sbjct: 343 IEGDKLK 349 >At3g62000.1 68416.m06963 O-methyltransferase family 3 protein several O-methyltransferases - different species; contains Pfam 01596 O-methyltransferase domain Length = 278 Score = 27.9 bits (59), Expect = 9.7 Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%) Frame = +3 Query: 174 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 353 V E+ S+ + + +A S + K+G + E++K +I+NG+ + DFA+ Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202 Query: 354 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 527 K + + YF + +++ + + N H + +++ I + K K+VS Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262 Query: 528 WKFTPV 545 P+ Sbjct: 263 ISMVPI 268 >At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family protein contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 362 Score = 27.9 bits (59), Expect = 9.7 Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 1/58 (1%) Frame = +3 Query: 495 DSKDKTSKKVSWKFTPVLENNRVYF-KIMSTEDKQYLKLDNTKGSSDDRIIYGDSTAD 665 D +++ KK+ WK E N+VY +I++++ K L+ K + YG S+++ Sbjct: 277 DMEEERDKKMFWKMYFAFEGNKVYCEQILNSDLKAELEKLKEKEYTLSDYYYGSSSSN 334 >At3g04160.1 68416.m00440 expressed protein ; expression supported by MPSS Length = 712 Score = 27.9 bits (59), Expect = 9.7 Identities = 11/37 (29%), Positives = 18/37 (48%) Frame = +2 Query: 281 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 391 SR ++E G +P DK WKG + + +P+ Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599 >At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein low similarity to SP|Q99614 Tetratricopeptide repeat protein 1 {Homo sapiens}; contains Pfam profiles PF00564: PB1 domain, PF00515: TPR Domain Length = 697 Score = 27.9 bits (59), Expect = 9.7 Identities = 17/49 (34%), Positives = 25/49 (51%) Frame = +3 Query: 192 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 338 YM + +GEY AI +C+ L E K +KR N +DFA++ Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,972,614 Number of Sequences: 28952 Number of extensions: 403928 Number of successful extensions: 1360 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 1307 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1359 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2129873112 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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