BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_I22
(909 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.17
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 1.2
SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|c... 28 1.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.9
SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 27 4.9
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.5 bits (68), Expect = 0.17
Identities = 22/77 (28%), Positives = 28/77 (36%)
Frame = +1
Query: 670 ALPXPXGASXXDEPPTSSXGHRTPYDEPDXHAGXSPHPXXFVPWVXXPXXGGXIPMSTXX 849
++P P GA PP + P +P A P P VP V P +P +
Sbjct: 1075 SIPAPSGA-----PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPS-V 1128
Query: 850 XXPPPXXPPPXPXXPXP 900
PP P P P P
Sbjct: 1129 AAPPVPVPSGAPPVPKP 1145
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/73 (26%), Positives = 21/73 (28%)
Frame = +1
Query: 682 PXGASXXDEPPTSSXGHRTPYDEPDXHAGXSPHPXXFVPWVXXPXXGGXIPMSTXXXXPP 861
P A PP P P A P P VP V P +P + P
Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPV 1229
Query: 862 PXXPPPXPXXPXP 900
P P P P
Sbjct: 1230 PVPTAKAPPVPAP 1242
Score = 26.2 bits (55), Expect = 6.4
Identities = 22/79 (27%), Positives = 26/79 (32%)
Frame = +1
Query: 664 AGALPXPXGASXXDEPPTSSXGHRTPYDEPDXHAGXSPHPXXFVPWVXXPXXGGXIPMST 843
A +P P GA +P ++ P P P P P V P G IP
Sbjct: 1130 APPVPVPSGAPPVPKPSVAAPPVPAPSGAPPV-----PKPSVAAPPVPAPSSG--IPPVP 1182
Query: 844 XXXXPPPXXPPPXPXXPXP 900
P PPP P P
Sbjct: 1183 KPAAGVPPVPPPSEAPPVP 1201
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.7 bits (61), Expect = 1.2
Identities = 25/90 (27%), Positives = 29/90 (32%), Gaps = 7/90 (7%)
Frame = +1
Query: 652 RXGXAGALPXPX-GASXXDEPP---TSSXGHRTPYDEPDXHAGXSPHPXXFVPW---VXX 810
R AG++P P G S PP S G + P P P +P
Sbjct: 344 RSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPAL 403
Query: 811 PXXGGXIPMSTXXXXPPPXXPPPXPXXPXP 900
P G ST PP PP P P
Sbjct: 404 PPLGNASRTSTPPVPTPPSLPPSAPPSLPP 433
>SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 297
Score = 28.3 bits (60), Expect = 1.6
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = +1
Query: 709 PPTSSXGHRTPYDEPDXHAGXSPHPXXF 792
P T GH TP+ + D H G H F
Sbjct: 137 PDTPDSGHSTPHSKGDVHPGMPQHNTRF 164
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +3
Query: 855 PPXXPPXPXPGSSXPLXP 908
PP PP P P SS P P
Sbjct: 1727 PPSAPPPPLPASSAPSVP 1744
>SPCC1223.08c |dfr1||dihydrofolate reductase
Dfr1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 461
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +2
Query: 551 GXAQGCGAGARMASKYPAARPPSP 622
G +QG G GA +A +PP+P
Sbjct: 110 GFSQGAGIGAMLAQMLQPGQPPNP 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,479,845
Number of Sequences: 5004
Number of extensions: 39972
Number of successful extensions: 106
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 87
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 460503700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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