BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_I22 (909 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.17 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 1.2 SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|c... 28 1.6 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.9 SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 27 4.9 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.5 bits (68), Expect = 0.17 Identities = 22/77 (28%), Positives = 28/77 (36%) Frame = +1 Query: 670 ALPXPXGASXXDEPPTSSXGHRTPYDEPDXHAGXSPHPXXFVPWVXXPXXGGXIPMSTXX 849 ++P P GA PP + P +P A P P VP V P +P + Sbjct: 1075 SIPAPSGA-----PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPS-V 1128 Query: 850 XXPPPXXPPPXPXXPXP 900 PP P P P P Sbjct: 1129 AAPPVPVPSGAPPVPKP 1145 Score = 28.7 bits (61), Expect = 1.2 Identities = 19/73 (26%), Positives = 21/73 (28%) Frame = +1 Query: 682 PXGASXXDEPPTSSXGHRTPYDEPDXHAGXSPHPXXFVPWVXXPXXGGXIPMSTXXXXPP 861 P A PP P P A P P VP V P +P + P Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPV 1229 Query: 862 PXXPPPXPXXPXP 900 P P P P Sbjct: 1230 PVPTAKAPPVPAP 1242 Score = 26.2 bits (55), Expect = 6.4 Identities = 22/79 (27%), Positives = 26/79 (32%) Frame = +1 Query: 664 AGALPXPXGASXXDEPPTSSXGHRTPYDEPDXHAGXSPHPXXFVPWVXXPXXGGXIPMST 843 A +P P GA +P ++ P P P P P V P G IP Sbjct: 1130 APPVPVPSGAPPVPKPSVAAPPVPAPSGAPPV-----PKPSVAAPPVPAPSSG--IPPVP 1182 Query: 844 XXXXPPPXXPPPXPXXPXP 900 P PPP P P Sbjct: 1183 KPAAGVPPVPPPSEAPPVP 1201 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 28.7 bits (61), Expect = 1.2 Identities = 25/90 (27%), Positives = 29/90 (32%), Gaps = 7/90 (7%) Frame = +1 Query: 652 RXGXAGALPXPX-GASXXDEPP---TSSXGHRTPYDEPDXHAGXSPHPXXFVPW---VXX 810 R AG++P P G S PP S G + P P P +P Sbjct: 344 RSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPAL 403 Query: 811 PXXGGXIPMSTXXXXPPPXXPPPXPXXPXP 900 P G ST PP PP P P Sbjct: 404 PPLGNASRTSTPPVPTPPSLPPSAPPSLPP 433 >SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 28.3 bits (60), Expect = 1.6 Identities = 11/28 (39%), Positives = 13/28 (46%) Frame = +1 Query: 709 PPTSSXGHRTPYDEPDXHAGXSPHPXXF 792 P T GH TP+ + D H G H F Sbjct: 137 PDTPDSGHSTPHSKGDVHPGMPQHNTRF 164 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.6 bits (56), Expect = 4.9 Identities = 10/18 (55%), Positives = 10/18 (55%) Frame = +3 Query: 855 PPXXPPXPXPGSSXPLXP 908 PP PP P P SS P P Sbjct: 1727 PPSAPPPPLPASSAPSVP 1744 >SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 461 Score = 26.6 bits (56), Expect = 4.9 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +2 Query: 551 GXAQGCGAGARMASKYPAARPPSP 622 G +QG G GA +A +PP+P Sbjct: 110 GFSQGAGIGAMLAQMLQPGQPPNP 133 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,479,845 Number of Sequences: 5004 Number of extensions: 39972 Number of successful extensions: 106 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 87 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 100 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 460503700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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