BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_I12 (897 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC354.09c |||Tre1 family protein |Schizosaccharomyces pombe|ch... 30 0.39 SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccha... 30 0.39 SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizos... 28 1.6 SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ... 28 1.6 SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 28 2.1 SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 28 2.1 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.7 SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosacchar... 27 2.7 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 27 2.7 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 27 4.8 SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces ... 26 6.3 SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces po... 26 6.3 SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c... 26 8.4 SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 26 8.4 SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharo... 26 8.4 >SPBC354.09c |||Tre1 family protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 794 Score = 30.3 bits (65), Expect = 0.39 Identities = 15/48 (31%), Positives = 24/48 (50%) Frame = +3 Query: 471 SIEIPGSNEPNKTVVISYDICAYGKSPGHSEVTFNVTPPGFVNLDTVY 614 S+ I G + + +++ SY + Y + TF +P GFVN VY Sbjct: 197 SLSIDGDDSFHPSLIESYQVGGYDGVSIPTPATFGGSPSGFVNAPLVY 244 >SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccharomyces pombe|chr 1|||Manual Length = 931 Score = 30.3 bits (65), Expect = 0.39 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = +1 Query: 106 YSYCXSNFYSNKLYL*LNSVFVFCERAVNRSRWRNV 213 Y + N YS ++L + VFC VN RW N+ Sbjct: 382 YDHKFRNLYSPNIFLSIVQALVFCGDMVNIQRWYNM 417 >SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 28.3 bits (60), Expect = 1.6 Identities = 12/45 (26%), Positives = 23/45 (51%), Gaps = 1/45 (2%) Frame = +2 Query: 599 LRHSVPTYNRHALQR-NKHNIVHNGLDILRGLVSFVLPSNIHKLQ 730 + HS+P H L+ HN N +D+L ++++V I ++ Sbjct: 258 VNHSLPNVRPHTLESVMPHNCTKNAMDLLHKMLTYVPSKRISAIE 302 >SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 28.3 bits (60), Expect = 1.6 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = +3 Query: 690 WSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFN 809 W V PQI N++ KS GL+ F+ ++ S++N Sbjct: 30 WCVQLIPQIIKNYRAKSTEGLDTLFILSWVVASIPLSVYN 69 Score = 27.9 bits (59), Expect = 2.1 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 2/48 (4%) Frame = +3 Query: 666 MGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMG--FTMYSL 803 MG + ++ F PQ F+ ++V G+++ FLA++ G F+ SL Sbjct: 145 MGVLATVLVNIGFLPQYISIFRARAVTGISYLFLAIDSSGSLFSFLSL 192 >SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual Length = 968 Score = 27.9 bits (59), Expect = 2.1 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = +2 Query: 617 TYNRHALQRNKHNIVHNGLDILRGLVSFVLPSNIHKLQ 730 +Y H L R +H+I+ N L+ + FV +N +Q Sbjct: 539 SYGNHVLNRIQHDILENHFCCLKNVKDFVCGANTISMQ 576 >SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 851 Score = 27.9 bits (59), Expect = 2.1 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +2 Query: 686 GLVSFVLPSNIHKLQKEKRSRSQLRFSGAKHN 781 G V++ SN+HK KEK SQ+R + K N Sbjct: 643 GRVAYSRISNLHKSSKEKGKDSQVRVALHKEN 674 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 27.5 bits (58), Expect = 2.7 Identities = 22/82 (26%), Positives = 32/82 (39%) Frame = +3 Query: 288 LILGIPSAHSQLDKMYVEVTDLNLLVEETRLLKLVQIGQYNTSLEVISNIQHPDIVQLVP 467 LILGIPS +Y + ++ E T + K S ++N P + Sbjct: 792 LILGIPSLQLVRPDIYDSIREMIASNENTEVEK-----DKLDSFSALANPASPKAPEYTF 846 Query: 468 PSIEIPGSNEPNKTVVISYDIC 533 SI +P N PN I +C Sbjct: 847 VSIHVPPLNVPNAEGEIEESVC 868 >SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 27.5 bits (58), Expect = 2.7 Identities = 13/50 (26%), Positives = 26/50 (52%) Frame = +3 Query: 636 SNEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMG 785 +N + +S +G + W V PQ+ N+K +S ++ FL + ++G Sbjct: 11 ANILTELSSFLGALSLGCWVVLLIPQLLENYKNQSGESISDLFLIIWLIG 60 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 27.5 bits (58), Expect = 2.7 Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 6/44 (13%) Frame = +2 Query: 524 RYMRVRKES-GTFGSDVQCY---TARFCQLR--HSVPTYNRHAL 637 RY VR GTFG +QCY T R C ++ ++P Y +L Sbjct: 361 RYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASL 404 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 26.6 bits (56), Expect = 4.8 Identities = 10/31 (32%), Positives = 20/31 (64%) Frame = +3 Query: 660 YIMGWIYFAAWSVSFYPQIYINFKRKSVVGL 752 Y++ W+ AAW + Y + Y ++R +++GL Sbjct: 102 YVLFWLSMAAWVLQLYARSY--WQRDTLLGL 130 >SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 318 Score = 26.2 bits (55), Expect = 6.3 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = -3 Query: 826 RKYSPQLKSEYMVKPI-MFSARKSKLRPTTLFLLKFM 719 RKY P L+S I F +K++LR T+ +LLK M Sbjct: 188 RKYDPFLRSRMFTLTIRYFLLQKNRLR-TSFYLLKHM 223 >SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 26.2 bits (55), Expect = 6.3 Identities = 14/32 (43%), Positives = 16/32 (50%) Frame = +3 Query: 348 DLNLLVEETRLLKLVQIGQYNTSLEVISNIQH 443 DLNL V +L LVQ GQ L I + H Sbjct: 358 DLNLQVYHEKLRNLVQQGQAAECLNTIKRMSH 389 >SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 25.8 bits (54), Expect = 8.4 Identities = 15/45 (33%), Positives = 21/45 (46%) Frame = +3 Query: 348 DLNLLVEETRLLKLVQIGQYNTSLEVISNIQHPDIVQLVPPSIEI 482 DL++LV E L L + + EVI HPD L+ +I Sbjct: 439 DLDILVTEQGLADLRGLSPTERAREVIDKCAHPDYKPLLREYFDI 483 >SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 1496 Score = 25.8 bits (54), Expect = 8.4 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +2 Query: 815 TVFSKEIQNEYFSRHPRGLNPVQLNDV 895 +VFS+E+Q +Y R G V NDV Sbjct: 547 SVFSEEVQEDYDERQFTGEAAVSANDV 573 >SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 25.8 bits (54), Expect = 8.4 Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 3/57 (5%) Frame = +3 Query: 171 VL*TSGKQITMA--ECSAVLRPRAAACSISSHRIRFCLVVMLILGIPSAH-SQLDKM 332 VL TS ++++ +C+A+ + A + S R L V LI P+AH SQ+ + Sbjct: 301 VLVTSPEEVSQVIEQCNAIRKTAATDFNTYSSRSHAILQVFLIRNNPTAHTSQISSL 357 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,523,466 Number of Sequences: 5004 Number of extensions: 74667 Number of successful extensions: 206 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 198 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 206 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 452494940 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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