Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_I12
(897 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40670.1 68418.m04937 PQ-loop repeat family protein / transme... 82 4e-16
At2g41050.1 68415.m05069 PQ-loop repeat family protein / transme... 39 0.005
At4g20100.1 68417.m02941 PQ-loop repeat family protein / transme... 37 0.016
At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme... 35 0.064
At5g39020.1 68418.m04722 protein kinase family protein contains ... 34 0.11
At1g17665.1 68414.m02187 expressed protein 31 1.4
At3g51550.1 68416.m05645 protein kinase family protein contains ... 30 2.4
At5g59470.1 68418.m07453 PQ-loop repeat family protein / transme... 29 3.2
At5g38260.1 68418.m04612 serine/threonine protein kinase, putati... 29 4.2
At4g07390.1 68417.m01134 PQ-loop repeat family protein / transme... 29 4.2
At5g54010.1 68418.m06718 glycosyltransferase family protein cont... 29 5.5
At3g09010.1 68416.m01055 protein kinase family protein contains ... 29 5.5
At1g26730.1 68414.m03255 EXS family protein / ERD1/XPR1/SYG1 fam... 29 5.5
At4g14140.1 68417.m02181 DNA (cytosine-5-)-methyltransferase (ME... 28 7.3
At3g54240.1 68416.m05995 hydrolase, alpha/beta fold family prote... 28 9.7
At1g71170.1 68414.m08212 6-phosphogluconate dehydrogenase NAD-bi... 28 9.7
>At5g40670.1 68418.m04937 PQ-loop repeat family protein /
transmembrane family protein similar to SP|O60931
Cystinosin {Homo sapiens}; contains Pfam profile
PF04193: PQ loop repeat
Length = 270
Score = 82.2 bits (194), Expect = 4e-16
Identities = 35/58 (60%), Positives = 47/58 (81%), Gaps = 1/58 (1%)
Frame = +3
Query: 654 ISY-IMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNCGLYF 824
ISY I+GWI FA+WS+SFYPQ+ +NF+R+SVVGLNFDF+ LN+ + Y ++N LYF
Sbjct: 11 ISYEIVGWIAFASWSISFYPQLILNFRRRSVVGLNFDFVMLNLTKHSSYMIYNVCLYF 68
>At2g41050.1 68415.m05069 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 376
Score = 38.7 bits (86), Expect = 0.005
Identities = 20/61 (32%), Positives = 34/61 (55%)
Frame = +3
Query: 630 MHSNEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFN 809
+HS+ + +S +G I +W V+ PQI N+ KS GL+ FL ++G +++L
Sbjct: 3 LHSSFRDGLSLSLGIISVISWGVAEIPQIMTNYSEKSTEGLSITFLTTWMIG-DIFNLLG 61
Query: 810 C 812
C
Sbjct: 62 C 62
Score = 28.7 bits (61), Expect = 5.5
Identities = 16/42 (38%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +3
Query: 633 HSN-EINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLN 755
HS E + I +GW A + PQI +N +R V GLN
Sbjct: 261 HSGGESSRIGMFLGWAMAAIYMGGRLPQICLNMRRGHVEGLN 302
>At4g20100.1 68417.m02941 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 288
Score = 37.1 bits (82), Expect = 0.016
Identities = 17/53 (32%), Positives = 32/53 (60%)
Frame = +3
Query: 654 ISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNC 812
+S +G I +WSV+ PQI N+ +KS+ G++ FL ++G ++++ C
Sbjct: 6 LSLSLGIISVISWSVAEIPQIMTNYNQKSIEGVSITFLTTWMLG-DIFNVVGC 57
Score = 29.5 bits (63), Expect = 3.2
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +3
Query: 615 LRITVMHSNEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLN 755
L ++ + E N I +GW A + PQI +N +R +V GLN
Sbjct: 166 LEVSSGNLGENNNIGMWLGWAMAAIYMGGRLPQICMNVRRGNVEGLN 212
>At4g36850.1 68417.m05225 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 374
Score = 35.1 bits (77), Expect = 0.064
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 654 ISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNC 812
+S+ +G W V+ PQ+ NF+ KS G++ FL + G +++L C
Sbjct: 33 VSFALGIASLLCWGVAEIPQVITNFRTKSSNGVSLSFLLAWVAG-DIFNLVGC 84
Score = 32.7 bits (71), Expect = 0.34
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 666 MGWIYFAAWSVSFYPQIYINFKRKSVVGLN 755
+GW+ A + PQI++N KR SV GLN
Sbjct: 262 LGWLMAAIYMGGRIPQIWLNIKRGSVEGLN 291
>At5g39020.1 68418.m04722 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 813
Score = 34.3 bits (75), Expect = 0.11
Identities = 18/45 (40%), Positives = 25/45 (55%)
Frame = -1
Query: 432 LISLLGMCYIGLFGPV*VVEFLRLTDSDQLLRRTSCLTGNVHSVY 298
++SLLG CY G + + EFL DQ + R LT NV ++Y
Sbjct: 551 IVSLLGFCYEGSKRAI-ISEFLEHGSLDQFISRNKSLTPNVTTLY 594
>At1g17665.1 68414.m02187 expressed protein
Length = 338
Score = 30.7 bits (66), Expect = 1.4
Identities = 21/86 (24%), Positives = 39/86 (45%), Gaps = 1/86 (1%)
Frame = +3
Query: 189 KQITMAECSAVLRPRAAACSISSHRIRFCLVVMLILGIPSAHSQLDKMYVEVTDLNLLVE 368
KQ+ E VL PR + SIS ++ F + + ++ + Y ++ N
Sbjct: 140 KQLGEGEDGLVLNPRTDSISISENKPEFVCLYPFTSTSSATQRKIKQQYDQLVKCN---- 195
Query: 369 ETRLLKLVQIGQY-NTSLEVISNIQH 443
+ L L Q+G++ N +E + +QH
Sbjct: 196 NAKGLTLAQVGEFANCLIEAKNELQH 221
>At3g51550.1 68416.m05645 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 895
Score = 29.9 bits (64), Expect = 2.4
Identities = 17/64 (26%), Positives = 32/64 (50%)
Frame = +3
Query: 396 IGQYNTSLEVISNIQHPDIVQLVPPSIEIPGSNEPNKTVVISYDICAYGKSPGHSEVTFN 575
+ ++ T +E++S ++H +V L+ G E N +++ YD A+G H T N
Sbjct: 575 VHEFQTEIEMLSKLRHRHLVSLI-------GYCEENCEMILVYDYMAHGTMREHLYKTQN 627
Query: 576 VTPP 587
+ P
Sbjct: 628 PSLP 631
>At5g59470.1 68418.m07453 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q60441
Mannose-P-dolichol utilization defect 1 protein
(Suppressor of Lec15 and Lec35 glycosylation mutation)
(SL15) {Cricetulus griseus}; contains Pfam profile
PF04193: PQ loop repeat
Length = 239
Score = 29.5 bits (63), Expect = 3.2
Identities = 19/54 (35%), Positives = 28/54 (51%)
Frame = +3
Query: 651 IISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNC 812
+IS ++G+ AA PQI KSV GL+ L ++G+T+ SL C
Sbjct: 29 LISKLLGYFLVAASMTVKLPQIMKIVDNKSVKGLSVVAFELEVIGYTI-SLAYC 81
>At5g38260.1 68418.m04612 serine/threonine protein kinase, putative
similar to receptor serine/threonine kinase PR55K
gi|1235680|gb|AAC49208; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 638
Score = 29.1 bits (62), Expect = 4.2
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = -1
Query: 432 LISLLGMCYIGLFGPV*VVEFLRLTDSDQLLRRTSCLTGNVHSVY 298
++SLLG CY G + V EFL DQ L L +V ++Y
Sbjct: 376 IVSLLGFCYEGSKRAI-VYEFLENGSLDQFLSEKKSLNLDVSTLY 419
>At4g07390.1 68417.m01134 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q60441
Mannose-P-dolichol utilization defect 1 protein
(Suppressor of Lec15 and Lec35 glycosylation mutation)
{Cricetulus griseus}, Lec35 protein [Cricetulus griseus]
GI:9858721; contains Pfam profile PF04193: PQ loop
repeat
Length = 235
Score = 29.1 bits (62), Expect = 4.2
Identities = 19/54 (35%), Positives = 29/54 (53%)
Frame = +3
Query: 651 IISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNC 812
+IS ++G+ AA PQI + KSV GL+ L ++G+T+ SL C
Sbjct: 29 LISKLLGYCLVAASITVKLPQIMKIVQHKSVRGLSVVAFELEVVGYTI-SLAYC 81
>At5g54010.1 68418.m06718 glycosyltransferase family protein
contains Pfam profile: PF00201 UDP-glucoronosyl and
UDP-glucosyl transferase
Length = 453
Score = 28.7 bits (61), Expect = 5.5
Identities = 24/81 (29%), Positives = 35/81 (43%)
Frame = +3
Query: 510 VVISYDICAYGKSPGHSEVTFNVTPPGFVNLDTVYLRITVMHSNEINIISYIMGWIYFAA 689
+ IS A PG S+ TPPG+ + V LR +N ++ +SY G
Sbjct: 134 ITISAACVAISFVPGRSQDDLGSTPPGYPS-SKVLLR--GHETNSLSFLSYPFG------ 184
Query: 690 WSVSFYPQIYINFKRKSVVGL 752
SFY +I I K V+ +
Sbjct: 185 DGTSFYERIMIGLKNCDVISI 205
>At3g09010.1 68416.m01055 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 393
Score = 28.7 bits (61), Expect = 5.5
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +3
Query: 402 QYNTSLEVISNIQHPDIVQLVPPSIE 479
++ T + +ISNI HP++V+L+ IE
Sbjct: 86 EFLTEINLISNIHHPNLVKLIGCCIE 111
>At1g26730.1 68414.m03255 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 750
Score = 28.7 bits (61), Expect = 5.5
Identities = 15/45 (33%), Positives = 27/45 (60%)
Frame = +1
Query: 85 PHLKILTYSYCXSNFYSNKLYL*LNSVFVFCERAVNRSRWRNVVQ 219
P+++++ SY S+ NKL L + S FV +NRS+ N+++
Sbjct: 295 PYMEMVDKSYLTSSDEINKLMLRVESTFVEHFAGLNRSKGMNLLR 339
>At4g14140.1 68417.m02181 DNA (cytosine-5-)-methyltransferase (METII)
nearly identical to cytosine-5 methyltransferase (METII)
[Arabidopsis thaliana] GI:6523846
Length = 1519
Score = 28.3 bits (60), Expect = 7.3
Identities = 22/80 (27%), Positives = 37/80 (46%)
Frame = +2
Query: 593 CQLRHSVPTYNRHALQRNKHNIVHNGLDILRGLVSFVLPSNIHKLQKEKRSRSQLRFSGA 772
C++R + ++ L +K + NG++ G +VLP+ I K +K SR G
Sbjct: 863 CKIRENEEERSKTKLNDSKTGFLSNGIEYHNGDFVYVLPNYITKDGLKKGSRRTTLKCG- 921
Query: 773 KHNGLHHVLAFQLWTVFSKE 832
++ GL + QL V E
Sbjct: 922 RNVGLKAFVVCQLLDVIVLE 941
>At3g54240.1 68416.m05995 hydrolase, alpha/beta fold family protein
low similarity to SP|P22862|ESTE_PSEFL Arylesterase (EC
3.1.1.2) (Aryl-ester hydrolase) {Pseudomonas
fluorescens}; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 350
Score = 27.9 bits (59), Expect = 9.7
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +3
Query: 483 PGSNEPNKTVVISYDICAYGKSPGHS 560
PG E N V+SYD YG+S HS
Sbjct: 91 PGFLEKNGIYVVSYDRPGYGESDPHS 116
>At1g71170.1 68414.m08212 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein contains Pfam
profile: PF03446 NAD binding domain of
6-phosphogluconate
Length = 299
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -3
Query: 592 KPGGVTLNVTSECPGL 545
KPGGVT+++TS PGL
Sbjct: 98 KPGGVTVDMTSSKPGL 113
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,973,473
Number of Sequences: 28952
Number of extensions: 373298
Number of successful extensions: 998
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 970
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 996
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2110422216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -