BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_H12 (922 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 29 0.93 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 27 4.9 SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharo... 27 4.9 >SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 29.1 bits (62), Expect = 0.93 Identities = 11/21 (52%), Positives = 16/21 (76%) Frame = +3 Query: 177 VQTTVKKIHAYSHHLFALRTL 239 ++ VKKIH ++H +F LRTL Sbjct: 63 LKVAVKKIHPFNHPVFCLRTL 83 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.6 bits (56), Expect = 4.9 Identities = 21/76 (27%), Positives = 34/76 (44%) Frame = +2 Query: 80 TRNVFKNFKMKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHA 259 +RN F+ FK ++ +AS G + D+ + + PPL PK+ HR A Sbjct: 298 SRN-FEKFKKSNKKTVLFTTDIASR--GLDIPNVDFVLQ---LDPPLD-PKSFSHRCGRA 350 Query: 260 GKCACCPACVTLLGEG 307 G+ + LL +G Sbjct: 351 GRAGRAGVAIVLLNDG 366 >SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 26.6 bits (56), Expect = 4.9 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 5/30 (16%) Frame = +3 Query: 156 RTVLWYVVQTTV-----KKIHAYSHHLFAL 230 R + WY + TT+ + I+AY HHLF + Sbjct: 109 RFINWYFLMTTLYYAYGESIYAYFHHLFIM 138 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,762,510 Number of Sequences: 5004 Number of extensions: 48131 Number of successful extensions: 132 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 468512460 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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