Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_H04
(894 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 88 1e-18
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 72 1e-13
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 68 2e-12
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 56 7e-09
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 53 6e-08
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 49 1e-06
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 38 0.002
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 37 0.003
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 28 2.1
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 2.7
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 27 2.7
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 27 4.8
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 6.3
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 88.2 bits (209), Expect = 1e-18
Identities = 38/77 (49%), Positives = 52/77 (67%)
Frame = +3
Query: 201 LSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQ 380
++K +I+ + ++V+FYAPWCGHCK+LAPEY AA +L E+ I L +VD T+E
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEG 84
Query: 381 DLAESYGVRGYPTLKFF 431
DL Y +RGYPTL F
Sbjct: 85 DLCSEYSIRGYPTLNVF 101
Score = 79.4 bits (187), Expect = 6e-16
Identities = 38/85 (44%), Positives = 60/85 (70%), Gaps = 1/85 (1%)
Frame = +3
Query: 180 TEXNVLVLSKANFETVI-STTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 356
++ +++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 357 KVDATQEQDLAESYGVRGYPTLKFF 431
K+DAT E D+ S + G+PT+ FF
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFF 433
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 71.7 bits (168), Expect = 1e-13
Identities = 30/70 (42%), Positives = 46/70 (65%)
Frame = +3
Query: 222 TVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYG 401
T+ ++ + L+EFYA WCGHCKSLAP Y + L E+ + + + K+DA D+A+ Y
Sbjct: 34 TIRASKKGALIEFYATWCGHCKSLAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYH 92
Query: 402 VRGYPTLKFF 431
+ G+PTL +F
Sbjct: 93 ITGFPTLIWF 102
Score = 68.5 bits (160), Expect = 1e-12
Identities = 32/82 (39%), Positives = 50/82 (60%), Gaps = 1/82 (1%)
Frame = +3
Query: 189 NVLVLSKANFETVISTTEY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVD 365
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y + K+ + E +++ K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNVEIVKIN 199
Query: 366 ATQEQDLAESYGVRGYPTLKFF 431
A D+ + V +PT+KFF
Sbjct: 200 ADVFADIGRLHEVASFPTIKFF 221
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 67.7 bits (158), Expect = 2e-12
Identities = 31/79 (39%), Positives = 42/79 (53%)
Frame = +3
Query: 189 NVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDA 368
N + L+ NF + LV FYAPWCG+CK L P Y K A+ L P+ DA
Sbjct: 32 NTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNL-HSLLPVTAVDCDA 90
Query: 369 TQEQDLAESYGVRGYPTLK 425
Q + + Y V+G+PT+K
Sbjct: 91 DQNRAVCSQYQVQGFPTIK 109
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 56.0 bits (129), Expect = 7e-09
Identities = 25/78 (32%), Positives = 46/78 (58%)
Frame = +3
Query: 198 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 377
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 378 QDLAESYGVRGYPTLKFF 431
+ +A GV+ PT FF
Sbjct: 64 RQIASGLGVKAMPTFVFF 81
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 52.8 bits (121), Expect = 6e-08
Identities = 29/82 (35%), Positives = 42/82 (51%)
Frame = +3
Query: 198 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 377
V S ++ T IS + +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 378 QDLAESYGVRGYPTLKFFXEWQ 443
D+A+ GV PT+ F + Q
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQ 99
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 48.8 bits (111), Expect = 1e-06
Identities = 21/74 (28%), Positives = 40/74 (54%)
Frame = +3
Query: 198 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 377
V + F++++ + ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 378 QDLAESYGVRGYPT 419
++A GV P+
Sbjct: 61 SEIAAEAGVHAMPS 74
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/59 (30%), Positives = 30/59 (50%)
Frame = +3
Query: 240 EYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYP 416
+ IL+ FYAPW CK + + + A++ K++A + D+AES+ V P
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 37.1 bits (82), Expect = 0.003
Identities = 25/88 (28%), Positives = 42/88 (47%), Gaps = 2/88 (2%)
Frame = +3
Query: 174 VPTEXNVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAE--EESPI 347
V T + + L+ + E+ +S + +++Y P CG CK L P + K E E S
Sbjct: 22 VQTLVSGVPLTDNDLESEVSKGTWF-IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNF 80
Query: 348 KLAKVDATQEQDLAESYGVRGYPTLKFF 431
+VD ++E L+ +R PTL +
Sbjct: 81 HFGEVDCSKE--LSSCANIRAVPTLYLY 106
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 27.9 bits (59), Expect = 2.1
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +3
Query: 279 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVR 407
+C L P +AA L + +SP L VDA + L S+GVR
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVR 223
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -1
Query: 627 RKVLALVELWSEKKPNTITVLASISSLACSAEVTST 520
+K +L + W + + LASISS A SA + ST
Sbjct: 673 KKAASLPQFWLSPRSHNTARLASISSFAKSAVMNST 708
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 27.5 bits (58), Expect = 2.7
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -2
Query: 878 YLIFPTKILEAVSCENSTIVGMLWTNTP 795
Y FP CE + + ++WT TP
Sbjct: 238 YFTFPVNSFSIDGCEYNNVKALVWTTTP 265
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 26.6 bits (56), Expect = 4.8
Identities = 16/59 (27%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
Frame = -2
Query: 866 PTKILEAVSCENSTIVG----MLWTNTPGIEQILSDFFVFIXDTFLFEVLEKHNIFIFS 702
P ++ + VS ++ + G M+ I I+ ++ VFI + FL +++ N+FI S
Sbjct: 819 PERVEKFVSVISAGLAGSSTHMISATIIAISSIVMEYKVFISEPFLVQLISTLNLFITS 877
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 370 VASTFASLIGDSSSASFVAALAYSGARDLQW 278
V++ F SL+G S FVA + R L+W
Sbjct: 1039 VSNIFKSLVGSMSCVEFVAEARETINRSLEW 1069
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,048,650
Number of Sequences: 5004
Number of extensions: 53992
Number of successful extensions: 171
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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