BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_G14 (929 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R6... 35 0.067 At1g50970.1 68414.m05730 membrane trafficking VPS53 family prote... 29 3.3 At3g50380.1 68416.m05511 expressed protein 28 7.7 >At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R65511, gb|T42324 and gb|T20569 come from this gene Length = 571 Score = 35.1 bits (77), Expect = 0.067 Identities = 24/100 (24%), Positives = 51/100 (51%) Frame = +2 Query: 194 LVSIAVSNANILQGTVDINRLQTILKDNLKSKDVGTLYYAVRGLKQLKADVPNICEDLKT 373 L ++A AN+ + + LQ ++N K +D L + + L+ ++ IC+D+K Sbjct: 427 LKALAEQEANMEKVVQESKLLQQEAEENSKLRDF--LMDRGQIVDTLQGEISVICQDVKL 484 Query: 374 IKYDVKNLEQVFYLTNLALLTNCQNSLKPEVLATPTQALD 493 +K +N + + + ++C +S+K VL P++ L+ Sbjct: 485 LKEKFENRVPLTKSISSSFTSSCGSSMKSLVLENPSERLN 524 >At1g50970.1 68414.m05730 membrane trafficking VPS53 family protein contains Pfam domain PF04100: Vps53-like, N-terminal Length = 569 Score = 29.5 bits (63), Expect = 3.3 Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 1/96 (1%) Frame = +2 Query: 326 KQLKADVPNICEDLKTIKYDVKNL-EQVFYLTNLALLTNCQNSLKPEVLATPTQALDKKD 502 +Q +A V +IC D+K + + KN+ V L+ L +L + L+ + + Sbjct: 81 EQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSAVQQLQVMTSKRQYKEAATQL 140 Query: 503 VTIQELYLAVYTLXALGKGTIYDXEDALKNLIQLLK 610 I EL L K I + + LKN+ Q+LK Sbjct: 141 EAINELCNHFKAYMDLPK--IMELREKLKNIKQILK 174 >At3g50380.1 68416.m05511 expressed protein Length = 3071 Score = 28.3 bits (60), Expect = 7.7 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = -2 Query: 265 YGLK-AIDIHSALENVGITYCYTH*DEQLYSKVHFQI 158 YG+K A+ I S ++ TYC + Q KVHF I Sbjct: 1974 YGVKKALSIESVKVSLTTTYCSVTSESQCVGKVHFLI 2010 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,683,358 Number of Sequences: 28952 Number of extensions: 323784 Number of successful extensions: 730 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 616 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 714 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2217402144 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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