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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_G14
         (929 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R6...    35   0.067
At1g50970.1 68414.m05730 membrane trafficking VPS53 family prote...    29   3.3  
At3g50380.1 68416.m05511 expressed protein                             28   7.7  

>At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966,
           gb|R65511, gb|T42324 and gb|T20569 come from this gene
          Length = 571

 Score = 35.1 bits (77), Expect = 0.067
 Identities = 24/100 (24%), Positives = 51/100 (51%)
 Frame = +2

Query: 194 LVSIAVSNANILQGTVDINRLQTILKDNLKSKDVGTLYYAVRGLKQLKADVPNICEDLKT 373
           L ++A   AN+ +   +   LQ   ++N K +D   L    + +  L+ ++  IC+D+K 
Sbjct: 427 LKALAEQEANMEKVVQESKLLQQEAEENSKLRDF--LMDRGQIVDTLQGEISVICQDVKL 484

Query: 374 IKYDVKNLEQVFYLTNLALLTNCQNSLKPEVLATPTQALD 493
           +K   +N   +    + +  ++C +S+K  VL  P++ L+
Sbjct: 485 LKEKFENRVPLTKSISSSFTSSCGSSMKSLVLENPSERLN 524


>At1g50970.1 68414.m05730 membrane trafficking VPS53 family protein
           contains Pfam domain PF04100: Vps53-like, N-terminal
          Length = 569

 Score = 29.5 bits (63), Expect = 3.3
 Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 1/96 (1%)
 Frame = +2

Query: 326 KQLKADVPNICEDLKTIKYDVKNL-EQVFYLTNLALLTNCQNSLKPEVLATPTQALDKKD 502
           +Q +A V +IC D+K + +  KN+   V  L+ L +L +    L+        +    + 
Sbjct: 81  EQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSAVQQLQVMTSKRQYKEAATQL 140

Query: 503 VTIQELYLAVYTLXALGKGTIYDXEDALKNLIQLLK 610
             I EL         L K  I +  + LKN+ Q+LK
Sbjct: 141 EAINELCNHFKAYMDLPK--IMELREKLKNIKQILK 174


>At3g50380.1 68416.m05511 expressed protein
          Length = 3071

 Score = 28.3 bits (60), Expect = 7.7
 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
 Frame = -2

Query: 265  YGLK-AIDIHSALENVGITYCYTH*DEQLYSKVHFQI 158
            YG+K A+ I S   ++  TYC    + Q   KVHF I
Sbjct: 1974 YGVKKALSIESVKVSLTTTYCSVTSESQCVGKVHFLI 2010


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,683,358
Number of Sequences: 28952
Number of extensions: 323784
Number of successful extensions: 730
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 714
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2217402144
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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