Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_G13
(893 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5; Obtectomera|... 157 4e-37
UniRef50_P50725 Cluster: Attacin-A precursor; n=14; Obtectomera|... 128 2e-28
UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria cunea... 125 1e-27
UniRef50_Q95NH6 Cluster: Attacin-C precursor [Contains: Immune-i... 48 3e-04
UniRef50_Q29QG5 Cluster: IP02686p; n=5; Sophophora|Rep: IP02686p... 41 0.037
UniRef50_Q5MGP9 Cluster: Defense protein 2; n=1; Lonomia obliqua... 40 0.065
UniRef50_P24490 Cluster: Sarcotoxin II-3 precursor; n=5; Sarcoph... 40 0.11
UniRef50_Q17FI3 Cluster: Antibacterial peptide, putative; n=1; A... 35 3.2
UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin ... 34 4.3
>UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5;
Obtectomera|Rep: Attacin-like protein - Antheraea
mylitta (Tasar silkworm)
Length = 230
Score = 157 bits (381), Expect = 4e-37
Identities = 76/120 (63%), Positives = 89/120 (74%)
Frame = +1
Query: 175 RARXXAGSFTVXSAGTSGAALKVPLTGNXKXVLSAIGSADFNDRHKLSAASAGXALDNVN 354
R R AG+ TV S GTSGAA+K+P+TGN LSAIGS DFNDR+KL AA+AG A DNVN
Sbjct: 43 RVRRQAGALTVNSDGTSGAAVKIPITGNENHKLSAIGSLDFNDRNKLGAATAGLAYDNVN 102
Query: 355 GHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAFAIRNSXXAIPXAPXFXXLGGG 534
GHG +LT T IPGFG+++ AGKVNLFHN+NH L+A AFA RN IP P F +GGG
Sbjct: 103 GHGATLTKTHIPGFGDKMTAAGKVNLFHNDNHDLNANAFATRNMPN-IPQVPNFNTVGGG 161
Score = 81.0 bits (191), Expect = 4e-14
Identities = 36/59 (61%), Positives = 43/59 (72%)
Frame = +2
Query: 530 GGVDYMFKXKVGASLSAXHSDVIXRNDYSXGGKLNLFRSPSXSXXXXAGXXKFDTPFXR 706
GGVDYMFK ++GAS SA H+D I RNDYS GGKLN+F++P+ S AG KFD P R
Sbjct: 160 GGVDYMFKDRIGASASAAHTDFINRNDYSLGGKLNIFKTPTTSLDFNAGWKKFDMPSYR 218
>UniRef50_P50725 Cluster: Attacin-A precursor; n=14;
Obtectomera|Rep: Attacin-A precursor - Trichoplusia ni
(Cabbage looper)
Length = 254
Score = 128 bits (308), Expect = 2e-28
Identities = 62/122 (50%), Positives = 79/122 (64%)
Frame = +1
Query: 169 PXRARXXAGSFTVXSAGTSGAALKVPLTGNXKXVLSAIGSADFNDRHKLSAASAGXALDN 348
P R GS T+ S G+ G KVP+ GN K VLSA+GS D ND+ K ++ G ALDN
Sbjct: 58 PRVRRQAQGSVTLNSDGSMGLGAKVPIVGNEKNVLSALGSVDLNDQLKPASRGMGLALDN 117
Query: 349 VNGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAFAIRNSXXAIPXAPXFXXLG 528
VNGHGLS+ +PGFG++L AG+VN+FHN+NH +SAKAF +N P P F +G
Sbjct: 118 VNGHGLSVMKETVPGFGDRLTGAGRVNVFHNDNHDISAKAFVTKNMPD-FPNVPNFNTVG 176
Query: 529 GG 534
GG
Sbjct: 177 GG 178
Score = 70.1 bits (164), Expect = 7e-11
Identities = 33/64 (51%), Positives = 40/64 (62%)
Frame = +2
Query: 530 GGVDYMFKXKVGASLSAXHSDVIXRNDYSXGGKLNLFRSPSXSXXXXAGXXKFDTPFXRX 709
GGVDYM+K KVGASL ++ + R DYS G LN+FRSP+ S AG KFDTP +
Sbjct: 177 GGVDYMYKNKVGASLGMANTPFLDRKDYSAMGNLNVFRSPTTSVDFNAGFKKFDTPVFKS 236
Query: 710 XGSP 721
P
Sbjct: 237 NWEP 240
>UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria
cunea|Rep: Putative attacin - Hyphantria cunea (Fall
webworm)
Length = 233
Score = 125 bits (302), Expect = 1e-27
Identities = 65/118 (55%), Positives = 76/118 (64%)
Frame = +1
Query: 175 RARXXAGSFTVXSAGTSGAALKVPLTGNXKXVLSAIGSADFNDRHKLSAASAGXALDNVN 354
RAR GS + TS A +K+PL G+ K VLSA+GS F+ LS+AS G ALDNV
Sbjct: 44 RARRQLGSVFLNPDSTSRANIKLPLAGSNKNVLSALGSVGFDANKHLSSASGGLALDNVR 103
Query: 355 GHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAFAIRNSXXAIPXAPXFXXLG 528
GHGLSLTGT IP FG QL AG++NLFHN NH L+A AF RN IP P F +G
Sbjct: 104 GHGLSLTGTHIPNFGNQLTGAGRLNLFHNQNHDLNANAFLTRN-MPTIPQVPNFNTVG 160
Score = 62.9 bits (146), Expect = 1e-08
Identities = 31/64 (48%), Positives = 38/64 (59%)
Frame = +2
Query: 530 GGVDYMFKXKVGASLSAXHSDVIXRNDYSXGGKLNLFRSPSXSXXXXAGXXKFDTPFXRX 709
G ++YMFK KVGASL A + + R DYS G LNLFR+PS S AG K +PF +
Sbjct: 160 GSLNYMFKNKVGASLGASRTPFLQRTDYSANGNLNLFRNPSTSLDFNAGVSKSVSPFMQS 219
Query: 710 XGSP 721
P
Sbjct: 220 SWLP 223
>UniRef50_Q95NH6 Cluster: Attacin-C precursor [Contains:
Immune-induced peptide 16 (DIM-16) (MPAC)]; n=21;
Sophophora|Rep: Attacin-C precursor [Contains:
Immune-induced peptide 16 (DIM-16) (MPAC)] - Drosophila
melanogaster (Fruit fly)
Length = 241
Score = 48.0 bits (109), Expect = 3e-04
Identities = 33/107 (30%), Positives = 48/107 (44%), Gaps = 4/107 (3%)
Frame = +1
Query: 175 RARXXAGSFTVXSAGTSGAAL---KVPLTGNXKXVLSAIGSADFNDRHKLSAASAGXALD 345
R + GS T +G + A L K T + + + + + + ++G L
Sbjct: 47 RRQVLGGSLTSNPSGGADARLDLSKAVGTPDHHVIGQVFAAGNTQTKPVSTPVTSGATLG 106
Query: 346 -NVNGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAFAIRN 483
N +GHGL LT T PG + NLF+N H L AKAFA +N
Sbjct: 107 YNNHGHGLELTKTHTPGVRDSFQQTATANLFNNGVHNLDAKAFASQN 153
Score = 40.3 bits (90), Expect = 0.065
Identities = 18/41 (43%), Positives = 27/41 (65%), Gaps = 2/41 (4%)
Frame = +1
Query: 331 GXALD--NVNGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNN 447
G ALD ++ GHG +LT IPG G+QL + G+ NL+ + +
Sbjct: 165 GAALDYSHIKGHGATLTHANIPGLGKQLELGGRANLWQSQD 205
>UniRef50_Q29QG5 Cluster: IP02686p; n=5; Sophophora|Rep: IP02686p -
Drosophila melanogaster (Fruit fly)
Length = 192
Score = 41.1 bits (92), Expect = 0.037
Identities = 20/42 (47%), Positives = 23/42 (54%)
Frame = +1
Query: 346 NVNGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAF 471
N NGH LSL I G G A + NLF +NN L+A AF
Sbjct: 64 NANGHALSLQHGHIEGVGSTTTAAAQANLFQSNNAALNATAF 105
>UniRef50_Q5MGP9 Cluster: Defense protein 2; n=1; Lonomia
obliqua|Rep: Defense protein 2 - Lonomia obliqua (Moth)
Length = 113
Score = 40.3 bits (90), Expect = 0.065
Identities = 19/54 (35%), Positives = 28/54 (51%)
Frame = +2
Query: 536 VDYMFKXKVGASLSAXHSDVIXRNDYSXGGKLNLFRSPSXSXXXXAGXXKFDTP 697
+DY++K K+ ASL HS ++ R D S GK+NL + G K +P
Sbjct: 42 LDYLYKDKLSASLGVAHSGLLDRTDLSALGKVNLLNDKNTRLDLFGGLTKSMSP 95
>UniRef50_P24490 Cluster: Sarcotoxin II-3 precursor; n=5;
Sarcophaga|Rep: Sarcotoxin II-3 precursor - Sarcophaga
peregrina (Flesh fly) (Boettcherisca peregrina)
Length = 294
Score = 39.5 bits (88), Expect = 0.11
Identities = 23/69 (33%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +1
Query: 274 SAIGSADFNDRHKLSAASAGXALD--NVNGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNN 447
+++ +D + + G LD + NGHGL+ TR G G Q V G LF +N+
Sbjct: 197 ASVFRSDVRQNNGFNFQKTGGMLDYSHANGHGLNAGLTRFSGIGNQANVGGYSTLFRSND 256
Query: 448 HXLSAKAFA 474
S KA A
Sbjct: 257 GLTSLKANA 265
>UniRef50_Q17FI3 Cluster: Antibacterial peptide, putative; n=1;
Aedes aegypti|Rep: Antibacterial peptide, putative -
Aedes aegypti (Yellowfever mosquito)
Length = 265
Score = 34.7 bits (76), Expect = 3.2
Identities = 18/50 (36%), Positives = 21/50 (42%)
Frame = +1
Query: 352 NGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAFAIRNSXXAIP 501
N H S T T PG G Q + G NLF ++ L AF R P
Sbjct: 134 NEHSFSATRTNQPGAGSQTRLDGSANLFKTPSNRLDLNAFKSRTQPVGSP 183
Score = 33.5 bits (73), Expect = 7.4
Identities = 19/49 (38%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = +1
Query: 328 AGXALDNVNGHGLSLTGTRIPGFGE-QLGVAGKVNLFHNNNHXLSAKAF 471
AG +N NGHG S R P E L G+ NL+ + N S AF
Sbjct: 190 AGLNWNNANGHGASAGFDRTPAIKETNLYARGRANLWQSKNRQTSLDAF 238
>UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin -
Musca domestica (House fly)
Length = 208
Score = 34.3 bits (75), Expect = 4.3
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +1
Query: 310 KLSAASAGXALDNVNGHGLSLTGTRIPGFG-EQLGVAGKVNLFHNNNHXLS 459
K + G ++ NGHG S+T +RIP + V GK NL+ + + S
Sbjct: 127 KFNTVGGGLDYNHANGHGASVTASRIPQLNMNTVDVTGKANLWKSADRATS 177
Score = 33.9 bits (74), Expect = 5.6
Identities = 21/63 (33%), Positives = 28/63 (44%)
Frame = +1
Query: 346 NVNGHGLSLTGTRIPGFGEQLGVAGKVNLFHNNNHXLSAKAFAIRNSXXAIPXAPXFXXL 525
N + G SL+ +R FG NLF N+ H L A AF R + + F +
Sbjct: 75 NADRFGGSLSHSRTDNFGSTFSQKLNANLFQNDKHKLDANAFHSRTN---LDNGFKFNTV 131
Query: 526 GGG 534
GGG
Sbjct: 132 GGG 134
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 460,609,550
Number of Sequences: 1657284
Number of extensions: 6451058
Number of successful extensions: 17276
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 16512
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17221
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 80751996367
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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