BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_E20 (888 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Sch... 142 6e-35 SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosac... 140 2e-34 SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr... 28 1.5 SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein... 26 6.2 >SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Schizosaccharomyces pombe|chr 1|||Manual Length = 127 Score = 142 bits (344), Expect = 6e-35 Identities = 65/122 (53%), Positives = 88/122 (72%) Frame = +3 Query: 138 IVKKRPQRFXXHQSXRXAKLKRXWRKPXGXDNXVRRRFKGQYLMPNIGYGSNKKXRHMLP 317 I+KKR + F HQS R ++ WRKP G D+ VRRRF+G MP IGYG+NKK R+ +P Sbjct: 6 IIKKRTKPFKRHQSDRFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCMP 65 Query: 318 NGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAXARLRS 497 NG + LV NV ++E+L+M N+ Y AEIA VS++KR IVE+A+ L ++VTNA A++RS Sbjct: 66 NGLKAFLVRNVSDVELLLMHNKTYAAEIAGNVSARKRVEIVEKARALGVKVTNAGAKVRS 125 Query: 498 QE 503 QE Sbjct: 126 QE 127 >SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosaccharomyces pombe|chr 2|||Manual Length = 127 Score = 140 bits (339), Expect = 2e-34 Identities = 65/122 (53%), Positives = 87/122 (71%) Frame = +3 Query: 138 IVKKRPQRFXXHQSXRXAKLKRXWRKPXGXDNXVRRRFKGQYLMPNIGYGSNKKXRHMLP 317 IVKKR + F HQS ++ WRKP G D+ VRRRF+G MP IGYG+NKK R+ +P Sbjct: 6 IVKKRTKPFKRHQSDLFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCMP 65 Query: 318 NGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAXARLRS 497 NG + LV NV ++E+L+M N+ Y AEIA VS++KR IVE+A+ L ++VTNA A++RS Sbjct: 66 NGLKAFLVRNVSDVELLLMHNKTYAAEIASNVSARKRVEIVEKARALGVKVTNAGAKVRS 125 Query: 498 QE 503 QE Sbjct: 126 QE 127 >SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 511 Score = 28.3 bits (60), Expect = 1.5 Identities = 13/41 (31%), Positives = 23/41 (56%) Frame = -2 Query: 506 ILLGAEASXRIRHSDAELLGSFHDQLPLLRRDTMSDLCAVL 384 +L ++ R+ HSD + + +F DQ +RR + LC +L Sbjct: 139 VLKNSKQISRLIHSDVQNISNFFDQNFGIRRSNIYQLCLLL 179 >SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein Mad3|Schizosaccharomyces pombe|chr 3|||Manual Length = 310 Score = 26.2 bits (55), Expect = 6.2 Identities = 10/31 (32%), Positives = 18/31 (58%) Frame = +2 Query: 251 QGSILDAQHWLRFQQEXPSYAPKWIP*GPSS 343 +G + A+ +LRFQQ+ + +W+ P S Sbjct: 175 KGKRMKAKPFLRFQQKYQQFTHRWLEFAPQS 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,146,004 Number of Sequences: 5004 Number of extensions: 31654 Number of successful extensions: 58 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 58 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 58 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 446488370 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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