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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_E17
         (890 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc...    27   4.7  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   4.7  
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    27   4.7  
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    26   6.3  
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p...    26   8.3  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    26   8.3  

>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 762

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -2

Query: 433 LGRALDVLPRLFQSLLGLAVRVSERXPGDSW 341
           +G  +  L RLF SL GL    ++  PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
 Frame = +1

Query: 178 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 297
           D   +F D++ H K FH   E+  +   +  +K   D  K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +1

Query: 172 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 261
           ++   DFFK +    ++ H TL ++ NSL+
Sbjct: 56  KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85


>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 19/64 (29%), Positives = 29/64 (45%)
 Frame = +1

Query: 85  PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 264
           P S S   + A    +      L +   V R    F + +EH+     K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229

Query: 265 SKDA 276
           SKDA
Sbjct: 230 SKDA 233


>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 394

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
 Frame = +1

Query: 142 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 318
           C  + +G  +RR  P +++ I      ++ KTL + FN+  + +++  + KA ++G   V
Sbjct: 13  CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72

Query: 319 LQQL 330
             Q+
Sbjct: 73  NNQI 76


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = -1

Query: 410 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 288
           AST+ K PWP  + L  +P     + +  CS+ +E  ++ +
Sbjct: 38  ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,493,334
Number of Sequences: 5004
Number of extensions: 40979
Number of successful extensions: 153
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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