BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_E16 (915 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.099 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 1.6 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 2.1 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 8.6 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 32.3 bits (70), Expect = 0.099 Identities = 23/69 (33%), Positives = 24/69 (34%) Frame = -1 Query: 891 GGGAXGKXPXTXPFXGVGPFXGPXGXXGFXXGXPXXWGKXXXXXGXXGXGXPXPXXRGXG 712 GGG+ G P F G G F G G G G G G G P G G Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG---PGGFGGG 250 Query: 711 XXGGGNGRG 685 G G G G Sbjct: 251 LGGFGGGPG 259 Score = 27.9 bits (59), Expect = 2.1 Identities = 23/66 (34%), Positives = 23/66 (34%) Frame = -1 Query: 840 GPFXGPXGXXGFXXGXPXXWGKXXXXXGXXGXGXPXPXXRGXGXXGGGNGRGPXXXTAFP 661 GP GP G GF G G G G G P G G GGG G F Sbjct: 199 GPPPGPGGFGGF--GGFGGEGHHHGGHGGFGGG-PGGFEGGPGGFGGGPGGFGGGLGGFG 255 Query: 660 RGGGXF 643 G G F Sbjct: 256 GGPGGF 261 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 28.3 bits (60), Expect = 1.6 Identities = 11/21 (52%), Positives = 11/21 (52%) Frame = +1 Query: 652 PPPGKGGXXXGPPPVSPPXXS 714 PPPG G PPP PP S Sbjct: 766 PPPGVAGAGPPPPPPPPPAVS 786 Score = 26.6 bits (56), Expect = 4.9 Identities = 14/50 (28%), Positives = 14/50 (28%) Frame = +2 Query: 743 PXPXXPXXXXXXPQXXGXPXXXPFXPXGPXXGPTPXXGXVXGXXPXAPPP 892 P P P P P P G P P G P PPP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781 Score = 26.6 bits (56), Expect = 4.9 Identities = 10/18 (55%), Positives = 10/18 (55%) Frame = +1 Query: 652 PPPGKGGXXXGPPPVSPP 705 PPP G GPPP PP Sbjct: 764 PPPPPGVAGAGPPPPPPP 781 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 27.9 bits (59), Expect = 2.1 Identities = 14/28 (50%), Positives = 17/28 (60%) Frame = -3 Query: 127 FEDDKYFNRFTVRLPCTSVRQNKKTKIL 44 FED +Y RFT+R T+V K KIL Sbjct: 634 FEDYQYMGRFTLRDQGTTVAVGKVVKIL 661 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.8 bits (54), Expect = 8.6 Identities = 13/36 (36%), Positives = 14/36 (38%), Gaps = 1/36 (2%) Frame = +1 Query: 655 PPGKGGXXXGPPPVS-PPXXSXPPXXRXXXXXSXPP 759 PP PPP+S PP S PP PP Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,947,762 Number of Sequences: 5004 Number of extensions: 22988 Number of successful extensions: 75 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 43 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 70 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 464508080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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