BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_E16
(915 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.099
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 1.6
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 2.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 8.6
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 32.3 bits (70), Expect = 0.099
Identities = 23/69 (33%), Positives = 24/69 (34%)
Frame = -1
Query: 891 GGGAXGKXPXTXPFXGVGPFXGPXGXXGFXXGXPXXWGKXXXXXGXXGXGXPXPXXRGXG 712
GGG+ G P F G G F G G G G G G G P G G
Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG---PGGFGGG 250
Query: 711 XXGGGNGRG 685
G G G G
Sbjct: 251 LGGFGGGPG 259
Score = 27.9 bits (59), Expect = 2.1
Identities = 23/66 (34%), Positives = 23/66 (34%)
Frame = -1
Query: 840 GPFXGPXGXXGFXXGXPXXWGKXXXXXGXXGXGXPXPXXRGXGXXGGGNGRGPXXXTAFP 661
GP GP G GF G G G G G P G G GGG G F
Sbjct: 199 GPPPGPGGFGGF--GGFGGEGHHHGGHGGFGGG-PGGFEGGPGGFGGGPGGFGGGLGGFG 255
Query: 660 RGGGXF 643
G G F
Sbjct: 256 GGPGGF 261
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.3 bits (60), Expect = 1.6
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +1
Query: 652 PPPGKGGXXXGPPPVSPPXXS 714
PPPG G PPP PP S
Sbjct: 766 PPPGVAGAGPPPPPPPPPAVS 786
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/50 (28%), Positives = 14/50 (28%)
Frame = +2
Query: 743 PXPXXPXXXXXXPQXXGXPXXXPFXPXGPXXGPTPXXGXVXGXXPXAPPP 892
P P P P P P G P P G P PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +1
Query: 652 PPPGKGGXXXGPPPVSPP 705
PPP G GPPP PP
Sbjct: 764 PPPPPGVAGAGPPPPPPP 781
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = -3
Query: 127 FEDDKYFNRFTVRLPCTSVRQNKKTKIL 44
FED +Y RFT+R T+V K KIL
Sbjct: 634 FEDYQYMGRFTLRDQGTTVAVGKVVKIL 661
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/36 (36%), Positives = 14/36 (38%), Gaps = 1/36 (2%)
Frame = +1
Query: 655 PPGKGGXXXGPPPVS-PPXXSXPPXXRXXXXXSXPP 759
PP PPP+S PP S PP PP
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,947,762
Number of Sequences: 5004
Number of extensions: 22988
Number of successful extensions: 75
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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