Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_E06
         (869 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   309   3e-85
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   308   5e-85
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate...    27   2.6  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    27   4.6  
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom...    26   8.0  

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  309 bits (759), Expect = 3e-85
 Identities = 153/220 (69%), Positives = 173/220 (78%), Gaps = 1/220 (0%)
 Frame = +3

Query: 132 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 308
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 309 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 488
           G LV++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65  GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124

Query: 489 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 668
           S DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM         
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTAT 184

Query: 669 XXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGXVIXLL 788
               DGPS K WR GRGA  NIIP+STGAAKAVG VI  L
Sbjct: 185 QKTVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPAL 224



 Score = 50.4 bits (115), Expect = 3e-07
 Identities = 24/35 (68%), Positives = 28/35 (80%)
 Frame = +2

Query: 758 QSCGXGYPALNGXLTGMAFRVPVANVSVVDLTVRL 862
           ++ G   PALNG LTGMAFRVP  +VSVVDLTV+L
Sbjct: 215 KAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTVKL 249


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  308 bits (757), Expect = 5e-85
 Identities = 152/220 (69%), Positives = 173/220 (78%), Gaps = 1/220 (0%)
 Frame = +3

Query: 132 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 308
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 309 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 488
           G LV++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65  GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124

Query: 489 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 668
           S DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM         
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTAT 184

Query: 669 XXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGXVIXLL 788
               DGPS K WR GRGA  NIIP+STGAAKAVG VI  L
Sbjct: 185 QKTVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPAL 224



 Score = 50.4 bits (115), Expect = 3e-07
 Identities = 24/35 (68%), Positives = 28/35 (80%)
 Frame = +2

Query: 758 QSCGXGYPALNGXLTGMAFRVPVANVSVVDLTVRL 862
           ++ G   PALNG LTGMAFRVP  +VSVVDLTV+L
Sbjct: 215 KAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTVKL 249


>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
           transferase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 453

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 15/45 (33%), Positives = 26/45 (57%)
 Frame = -2

Query: 190 SIEARSTKRPMRPNPLIPIFDMLSASYRKFVE*LTTTTYAQVQDG 56
           ++ A+ TK P+RP  + P   + SA + ++   +  T  AQVQ+G
Sbjct: 179 ALAAKLTK-PIRPLWVTPASRLTSAQFEEYFTVVLVTASAQVQNG 222


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
 Frame = -1

Query: 338 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 183
           +F  + N+ESIL+L   L +     + E I++IV+   +D R     HLS L  +
Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392


>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 15/62 (24%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
 Frame = -1

Query: 497 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPWNGLRVPF*E-NGNFVTVN 321
           + +G  +N  +S    +  C +C G     + ++ +P F     +R+P    NGN  T+ 
Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGFR--QQMRMPCNACNGNGRTIK 210

Query: 320 NK 315
           +K
Sbjct: 211 HK 212


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,437,439
Number of Sequences: 5004
Number of extensions: 72994
Number of successful extensions: 220
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)