Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_E05
         (895 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   253   2e-68
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   249   4e-67
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   126   4e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   114   2e-26
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce...    26   6.3  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  253 bits (620), Expect = 2e-68
 Identities = 112/173 (64%), Positives = 135/173 (78%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGXMPTDKTIGGGXDSFNTFFSETGAGK 329
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +         F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 330 XVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGXYTIGKEIVDLVLD 509
            VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARG YT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 510 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFXIYP 668
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y  KSKL+F +YP
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP 173



 Score = 90.6 bits (215), Expect = 3e-19
 Identities = 44/70 (62%), Positives = 50/70 (71%)
 Frame = +1

Query: 667 PXPQVSTXVVEPYNSILTTHTXLEHSDCAFMVDXXAIYDICRR*SXH*APTXTNLNRLXG 846
           P PQVST VVEPYNS+LTTH  L+ +DC FMVD  + YDICRR      P+  NLNRL  
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIA 232

Query: 847 QIVSSIXASL 876
           Q+VSSI ASL
Sbjct: 233 QVVSSITASL 242


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  249 bits (610), Expect = 4e-67
 Identities = 114/178 (64%), Positives = 138/178 (77%), Gaps = 5/178 (2%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGXMPTD-----KTIGGGXDSFNTFFSE 314
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K      D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 315 TGAGKXVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGXYTIGKEIV 494
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARG YT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 495 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFXIYP 668
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YG KS L+F +YP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP 177



 Score = 96.3 bits (229), Expect = 5e-21
 Identities = 47/70 (67%), Positives = 51/70 (72%)
 Frame = +1

Query: 667 PXPQVSTXVVEPYNSILTTHTXLEHSDCAFMVDXXAIYDICRR*SXH*APTXTNLNRLXG 846
           P PQVST VVEPYNS+LTTH  L++SDC FMVD  A YDICRR      PT  NLNRL  
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIA 236

Query: 847 QIVSSIXASL 876
           Q+VSSI ASL
Sbjct: 237 QVVSSITASL 246


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  126 bits (304), Expect = 4e-30
 Identities = 63/173 (36%), Positives = 90/173 (52%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGXMPTDKTIGGGXDSFNTFFSETGAGK 329
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 330 XVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGXYTIGKEIVDLVLD 509
            VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+G YT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 510 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFXIYP 668
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F + P
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAP 171



 Score = 46.0 bits (104), Expect = 7e-06
 Identities = 23/69 (33%), Positives = 37/69 (53%)
 Frame = +1

Query: 667 PXPQVSTXVVEPYNSILTTHTXLEHSDCAFMVDXXAIYDICRR*SXH*APTXTNLNRLXG 846
           P P+ S  VVEPYN+ L+ H  +E+SD  F +D  A+  I        +P+  +LN L  
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230

Query: 847 QIVSSIXAS 873
            +++ +  S
Sbjct: 231 AVMAGVTTS 239


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  114 bits (274), Expect = 2e-26
 Identities = 61/174 (35%), Positives = 95/174 (54%), Gaps = 2/174 (1%)
 Frame = +3

Query: 153 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGXMPTDKTIGGGXDSFNTFFSETGAGKX 332
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   + 
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 333 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGXYTIGKEIVDLVL 506
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 507 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFXIYP 668
           D I + AD    L+GF + H             L+ERL+  Y  K    + ++P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173



 Score = 30.7 bits (66), Expect = 0.29
 Identities = 19/66 (28%), Positives = 31/66 (46%)
 Frame = +1

Query: 679 VSTXVVEPYNSILTTHTXLEHSDCAFMVDXXAIYDICRR*SXH*APTXTNLNRLXGQIVS 858
           VS  VV+PYNS+L       ++D   ++D  A+  I         PT    N+L   ++S
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMS 237

Query: 859 SIXASL 876
           +   +L
Sbjct: 238 ASTTTL 243


>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2100

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 16/68 (23%), Positives = 31/68 (45%)
 Frame = -3

Query: 581  TSTEGVEDQESLQTGTLVSELADSVQNQIYDFLSNGVXTTGIVVGRIFLTSNKLFWMKQL 402
            TS  G+    +  T  L S L ++V+N+      N      I++G++F  + ++ +    
Sbjct: 1329 TSLNGLRTDSADSTDALNSNLNNTVENEANQTALNYARNLLIIIGQLFQLAAQMPYTSMQ 1388

Query: 401  SVCASADL 378
             V A  +L
Sbjct: 1389 EVPADHEL 1396


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,111,766
Number of Sequences: 5004
Number of extensions: 60108
Number of successful extensions: 142
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)