Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_D11
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 90 4e-19
SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 46 7e-06
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 34 0.024
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 28 1.5
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 28 2.0
SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 27 2.7
SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch... 27 2.7
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 27 4.7
SPBC691.02c |||RINT1 family protein|Schizosaccharomyces pombe|ch... 27 4.7
SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase Ggt2|Schizosacc... 26 6.2
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 26 6.2
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe... 26 8.2
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 89.8 bits (213), Expect = 4e-19
Identities = 42/92 (45%), Positives = 59/92 (64%), Gaps = 1/92 (1%)
Frame = +1
Query: 388 LQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPI 567
+Q R+ +Y H + M+ + A M+ T+LY KRFFPYYV +AG+D +GKG +YS+DP+
Sbjct: 72 IQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDKEGKGEIYSFDPV 131
Query: 568 GHCARHNFRAGGSAAAQLQPLLE-QSNWLKEY 660
G R RAGGSAA + P L+ Q N +Y
Sbjct: 132 GSYEREWCRAGGSAANFITPFLDNQVNLHNQY 163
Score = 66.9 bits (156), Expect = 4e-12
Identities = 29/66 (43%), Positives = 44/66 (66%)
Frame = +3
Query: 180 QHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCW 359
Q +F+PY NGG+ VAIAGD +A++ DTR G++I TR Q ++ ++ + V+GA+G
Sbjct: 3 QSQFDPYVQNGGTTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFE 62
Query: 360 CDTLTL 377
D L L
Sbjct: 63 ADALAL 68
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = +3
Query: 717 DVFISAAERDIYTGDSIYILIITAHGIQEEKFELRKD 827
D F SA ER I GDS+ + IIT G++ L+KD
Sbjct: 189 DAFTSAGERHIEVGDSVLVKIITKEGVETRIIPLKKD 225
>SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 204
Score = 46.0 bits (104), Expect = 7e-06
Identities = 25/86 (29%), Positives = 44/86 (51%), Gaps = 2/86 (2%)
Frame = +1
Query: 385 LLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDP 564
L + ++ +Y+ + + A ++S+ LY KRF PY+ V+AG+ +D + +D
Sbjct: 66 LFRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVSNDNTPFICGFDS 125
Query: 565 IGHCA--RHNFRAGGSAAAQLQPLLE 636
IG C +F G+A QL + E
Sbjct: 126 IG-CIDFAEDFIVSGTATEQLYGMCE 150
Score = 43.2 bits (97), Expect = 5e-05
Identities = 23/57 (40%), Positives = 30/57 (52%)
Frame = +3
Query: 207 NGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTL 377
NGGS VA+AG + I +D RL T + K+F + + T LG TG D TL
Sbjct: 7 NGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDVQTL 63
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 34.3 bits (75), Expect = 0.024
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = +3
Query: 210 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDT 368
G +IV + D V+GADTR + G I ++ KKL +S + G DT
Sbjct: 35 GTTIVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADT 87
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -2
Query: 418 RVRTSASSLVEDAXKVNVSHQQPVAPNTVLSDN 320
R++TSA+ LVE K S+ + + PN S N
Sbjct: 588 RIKTSANDLVETLMKCQPSYIRTIKPNQTKSPN 620
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +3
Query: 135 SVNENFPEYAVPGAKQHRFEPYADNGGSIVAIAGD-DYAVIGADT 266
S N F P K ++ +GG+ AIA D YA +G+DT
Sbjct: 118 SENNLFGRTVNPVVKDSNYDVGGSSGGAAAAIAADICYASVGSDT 162
>SPAC6C3.06c |||P-type ATPase, calcium
transporting|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1033
Score = 27.5 bits (58), Expect = 2.7
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 401 CRCTNTSITNL*PLRQSHRCSQLCCI 478
CRCT T N+ L Q + + +CCI
Sbjct: 743 CRCTPTQKANMTRLIQEKKQASVCCI 768
>SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 775
Score = 27.5 bits (58), Expect = 2.7
Identities = 18/90 (20%), Positives = 38/90 (42%)
Frame = +3
Query: 69 HSIRSISKRLKLIFP*KSYKMLSVNENFPEYAVPGAKQHRFEPYADNGGSIVAIAGDDYA 248
H +++ K +F K + + + P A+P + D + I GDD
Sbjct: 318 HLLKTAEKDNDFVFHDLVVKEVELPKISPLQALPPLPLEKLYGSQDGFETAKKIVGDDLF 377
Query: 249 VIGADTRLSTGFSIYTRDQKKLFKLSESTV 338
+ ++T S+Y+ + K+F+ ++ V
Sbjct: 378 KAFVPSAVTTASSLYSEETAKVFRAEQAEV 407
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.6 bits (56), Expect = 4.7
Identities = 20/77 (25%), Positives = 30/77 (38%)
Frame = +1
Query: 385 LLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDP 564
LL+ + MY + + A + S M Y + ++AG D G VYS
Sbjct: 82 LLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNM-LSAGLIVAGYDEKTGGDVYSIPL 140
Query: 565 IGHCARHNFRAGGSAAA 615
G + GGS +A
Sbjct: 141 GGSLHKQPLAIGGSGSA 157
>SPBC691.02c |||RINT1 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 678
Score = 26.6 bits (56), Expect = 4.7
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +1
Query: 610 AAQLQPLLEQSNWLKEYAECNRSS 681
+ QL+P+++ NWL+ + E + SS
Sbjct: 396 SVQLKPMMQYVNWLEAFYESHESS 419
>SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase
Ggt2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 611
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -1
Query: 302 VSGIYGKSSAETSVCSDNGIIIAGDGYN 219
V G G ++E VCSD G+ + DG N
Sbjct: 77 VRGRRGAVASEVPVCSDIGVSMLADGGN 104
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +3
Query: 225 AIAGDDYAVIGADTRLSTGFSI 290
AI GD Y+ IG++ GFSI
Sbjct: 321 AIVGDTYSTIGSNVEALPGFSI 342
>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 377
Score = 25.8 bits (54), Expect = 8.2
Identities = 22/77 (28%), Positives = 30/77 (38%)
Frame = +1
Query: 406 MYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIGHCARH 585
++ E S TPAV + + P VS VL G+DS + V P+ R
Sbjct: 105 LFGREKQNSEATPAVQPPATCPMSNSNQKPAGVSEVLTGVDSKQQSYVPEGCPVATPKRG 164
Query: 586 NFRAGGSAAAQLQPLLE 636
F G+ Q Q E
Sbjct: 165 WFNWFGNNDDQKQEAYE 181
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,497,791
Number of Sequences: 5004
Number of extensions: 72878
Number of successful extensions: 236
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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