Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_D11
(887 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 105 5e-23
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 44 1e-04
At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2) id... 43 2e-04
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 33 0.25
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 33 0.33
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 33 0.33
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 30 2.4
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 29 3.1
At4g26920.1 68417.m03874 hypothetical protein 29 3.1
At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-... 29 4.1
At4g04680.1 68417.m00684 hypothetical protein 29 5.4
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 28 9.5
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 28 9.5
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 28 9.5
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 28 9.5
At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1) (P... 28 9.5
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 105 bits (251), Expect = 5e-23
Identities = 43/85 (50%), Positives = 62/85 (72%)
Frame = +1
Query: 382 RLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYD 561
++L++R +Y+H+HNK M+ PA+AQ+LS LY+KRFFPYY NVL GLD +GKGCV++YD
Sbjct: 72 KVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDEEGKGCVFTYD 131
Query: 562 PIGHCARHNFRAGGSAAAQLQPLLE 636
+G R + A GS + + P L+
Sbjct: 132 AVGSYERVGYGAQGSGSTLIMPFLD 156
Score = 75.4 bits (177), Expect = 5e-14
Identities = 36/68 (52%), Positives = 47/68 (69%), Gaps = 1/68 (1%)
Frame = +3
Query: 177 KQH-RFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATG 353
KQH + PY +NGG+ VAIAG DY VI ADTR+STG+SI +RD K+ KL++ VL ++G
Sbjct: 3 KQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSG 62
Query: 354 CWCDTLTL 377
D L
Sbjct: 63 FQADVKAL 70
Score = 48.4 bits (110), Expect = 6e-06
Identities = 22/36 (61%), Positives = 27/36 (75%)
Frame = +3
Query: 720 VFISAAERDIYTGDSIYILIITAHGIQEEKFELRKD 827
VF SA ERDIYTGD + I+I+ A GI+ E +LRKD
Sbjct: 188 VFASATERDIYTGDKLEIMILKADGIKTELMDLRKD 223
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 44.4 bits (100), Expect = 1e-04
Identities = 21/58 (36%), Positives = 31/58 (53%)
Frame = +1
Query: 397 RMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIG 570
R ++Y+ + M A ++S +LY KRF PY V+AGL D K + + D IG
Sbjct: 70 RHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIAGLGDDDKPFICTMDSIG 127
>At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2)
identical to residues 14-204 of 20S proteasome beta
subunit PBC2 GB:AAC32069 [Arabidopsis thaliana]
Length = 204
Score = 43.2 bits (97), Expect = 2e-04
Identities = 20/58 (34%), Positives = 31/58 (53%)
Frame = +1
Query: 397 RMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIG 570
R ++Y+ + M A ++S +LY KRF P+ V+AGL D K + + D IG
Sbjct: 70 RHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQPVIAGLGDDNKPFICTMDSIG 127
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 33.1 bits (72), Expect = 0.25
Identities = 15/53 (28%), Positives = 30/53 (56%)
Frame = +3
Query: 210 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDT 368
G +I+ + + V+GAD+R STG + R K+ +L+++ + +G D+
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADS 64
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 32.7 bits (71), Expect = 0.33
Identities = 18/76 (23%), Positives = 34/76 (44%)
Frame = +1
Query: 382 RLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYD 561
R L + +++E + + ++ +++L+ MLY R V ++AG D G G Y +
Sbjct: 114 RNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDN 173
Query: 562 PIGHCARHNFRAGGSA 609
G F G +
Sbjct: 174 EGGRLKGDRFSVGSGS 189
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 32.7 bits (71), Expect = 0.33
Identities = 18/76 (23%), Positives = 34/76 (44%)
Frame = +1
Query: 382 RLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYD 561
R L + +++E + + ++ +++L+ MLY R V ++AG D G G Y +
Sbjct: 114 RNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDN 173
Query: 562 PIGHCARHNFRAGGSA 609
G F G +
Sbjct: 174 EGGRLKGDRFSVGSGS 189
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 29.9 bits (64), Expect = 2.4
Identities = 26/108 (24%), Positives = 47/108 (43%), Gaps = 6/108 (5%)
Frame = +1
Query: 334 QYWERLAAGVTH*PXTRLLQARMQMYEHEHNKSMTTPA--VAQMLS--TMLYYKRFF--P 495
+Y LA G+T + + QAR + E PA +A+ ++ + +Y + + P
Sbjct: 74 KYLGLLATGMTADSRSLVQQARNEAAEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRP 133
Query: 496 YYVSNVLAGLDSDGKGCVYSYDPIGHCARHNFRAGGSAAAQLQPLLEQ 639
V ++ G+D + +Y DP GH H + G + LE+
Sbjct: 134 LGVVAMVLGIDEERGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEK 181
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 29.5 bits (63), Expect = 3.1
Identities = 25/108 (23%), Positives = 47/108 (43%), Gaps = 6/108 (5%)
Frame = +1
Query: 334 QYWERLAAGVTH*PXTRLLQARMQMYEHE--HNKSMTTPAVAQMLS--TMLYYKRFF--P 495
+Y +A G+T + + QAR Q E + M +A+ ++ + +Y + + P
Sbjct: 74 KYIGLVATGITADARSLVQQARNQAAEFRFTYGYEMPVDILAKWIADKSQVYTQHAYMRP 133
Query: 496 YYVSNVLAGLDSDGKGCVYSYDPIGHCARHNFRAGGSAAAQLQPLLEQ 639
V ++ G+D + +Y DP GH H + G + LE+
Sbjct: 134 LGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEK 181
>At4g26920.1 68417.m03874 hypothetical protein
Length = 461
Score = 29.5 bits (63), Expect = 3.1
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 454 QMLSTMLYYKRFFPYYVSNV 513
+ML+ YY RFFP+Y NV
Sbjct: 93 EMLTLNEYYSRFFPWYAKNV 112
>At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-2)
similar to topoisomerase 6 subunit A (spo11)
[Arabidopsis thaliana] GI:12331186; contains Pfam
profile PF04406: Type IIB DNA topoisomerase; identical
to cDNA putative topoisomerase VIA (SPO11 gene 2)
GI:7270976
Length = 383
Score = 29.1 bits (62), Expect = 4.1
Identities = 12/49 (24%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = -1
Query: 287 GKSSAETSVCSDNGIIIAGD-GYNTTSVVRVGLETMLFRTRHSIFRKVL 144
GK + + S C +G I GD +++R ++ +H+IF +++
Sbjct: 178 GKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLV 226
>At4g04680.1 68417.m00684 hypothetical protein
Length = 261
Score = 28.7 bits (61), Expect = 5.4
Identities = 15/47 (31%), Positives = 22/47 (46%)
Frame = -2
Query: 712 RRAKGLFPSQRSFCYILHIL*ANLIVRGAVAAVQQRNHQRGSYGAHS 572
R LFPS FC+ L L A+ + +G + Q +H G H+
Sbjct: 193 RNRNFLFPSIYRFCFFLLNLRAHTMAKGFLDTGQSPSHNLGQGSTHT 239
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = +3
Query: 198 YADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDT 368
+ G +IV + D ++GADTR + G + ++ +K+ ++ + G DT
Sbjct: 35 FLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = +3
Query: 198 YADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDT 368
+ G +IV + D ++GADTR + G + ++ +K+ ++ + G DT
Sbjct: 35 FLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = +3
Query: 198 YADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDT 368
+ G +IV + D ++GADTR + G + ++ +K+ ++ + G DT
Sbjct: 35 FLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = +3
Query: 198 YADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDT 368
+ G +IV + D ++GADTR + G + ++ +K+ ++ + G DT
Sbjct: 35 FLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
>At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1)
(PRGB) identical to GB:CAA74026 from [Arabidopsis
thaliana] ( FEBS Lett. (1997) 416 (3), 281-285);
identical to cDNA proteasome subunit prgb GI:2511589
Length = 204
Score = 27.9 bits (59), Expect = 9.5
Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = +1
Query: 379 TRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYK-RFFPYYVSNVLAGLDSDGKGCVYS 555
T +Q + +Y+ + +TT A A L R PY V+ ++AG D + +Y
Sbjct: 57 TEYVQKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYY 116
Query: 556 YDPI 567
D I
Sbjct: 117 IDYI 120
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,175,089
Number of Sequences: 28952
Number of extensions: 379885
Number of successful extensions: 969
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 935
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 969
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2081245872
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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