Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_D07
         (903 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   256   4e-69
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   250   2e-67
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   126   6e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   110   3e-25

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  256 bits (626), Expect = 4e-69
 Identities = 111/170 (65%), Positives = 136/170 (80%)
 Frame = +3

Query: 177 HVGQAGVQLGNACWXLYCLEHGIQPDGQMPTDKXIGGGXDSFNTFFSETGAGKHVPRAVF 356
           HVGQAG Q+GNACW LYCLEHGIQP+G M  +         F+TFFSETG GK+VPR+++
Sbjct: 8   HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67

Query: 357 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 536
           VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68  VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127

Query: 537 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQV 686
            C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177



 Score = 86.2 bits (204), Expect = 6e-18
 Identities = 39/56 (69%), Positives = 45/56 (80%)
 Frame = +1

Query: 736 LEHSDCAFMVDNEAIYDICRRNLDIERPTXTNLNRFIGQIVSSXXASLKFXGPLNV 903
           L+ +DC FMVDNE+ YDICRRNLDIERP+  NLNR I Q+VSS  ASL+F G LNV
Sbjct: 195 LDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNV 250


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  250 bits (612), Expect = 2e-67
 Identities = 112/175 (64%), Positives = 138/175 (78%), Gaps = 5/175 (2%)
 Frame = +3

Query: 177 HVGQAGVQLGNACWXLYCLEHGIQPDGQMPTD-----KXIGGGXDSFNTFFSETGAGKHV 341
           HVGQAGVQ+GNACW LYCLEHGI PDG  PT+     K      D F TFFSETG GK V
Sbjct: 8   HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66

Query: 342 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 521
           PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67  PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126

Query: 522 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQV 686
           R++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAPQV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQV 181



 Score = 91.9 bits (218), Expect = 1e-19
 Identities = 42/56 (75%), Positives = 46/56 (82%)
 Frame = +1

Query: 736 LEHSDCAFMVDNEAIYDICRRNLDIERPTXTNLNRFIGQIVSSXXASLKFXGPLNV 903
           L++SDC FMVDNEA YDICRRNLDIERPT  NLNR I Q+VSS  ASL+F G LNV
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNV 254


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  126 bits (303), Expect = 6e-30
 Identities = 63/176 (35%), Positives = 94/176 (53%), Gaps = 2/176 (1%)
 Frame = +3

Query: 162 LHSLXHV--GQAGVQLGNACWXLYCLEHGIQPDGQMPTDKXIGGGXDSFNTFFSETGAGK 335
           +  + H+  GQ G Q+G A W     EHG+   G            +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSE--AQHERLNVYFNEAAGGK 58

Query: 336 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 515
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 516 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 683
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  110 bits (264), Expect = 3e-25
 Identities = 58/169 (34%), Positives = 92/169 (54%), Gaps = 2/169 (1%)
 Frame = +3

Query: 183 GQAGVQLGNACWXLYCLEHGIQPDGQMPTDKXIGGGXDSFNTFFSETGAGKHVPRAVFVD 362
           GQ G Q+G+  W   CLEHGI PDG +  +     G D  + FF ++   +++PRA+ +D
Sbjct: 11  GQCGNQIGSQFWQQLCLEHGIGPDGTL--ESFATEGVDRKDVFFYQSDDTRYIPRAILID 68

Query: 363 LEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLAD 536
           LEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++D I + AD
Sbjct: 69  LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127

Query: 537 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 683
               L+GF + H             L+ERL+  Y KK    ++++P  Q
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176



 Score = 32.3 bits (70), Expect = 0.097
 Identities = 13/53 (24%), Positives = 28/53 (52%)
 Frame = +1

Query: 742 HSDCAFMVDNEAIYDICRRNLDIERPTXTNLNRFIGQIVSSXXASLKFXGPLN 900
           ++D   ++DN A+  I    L  + PT    N+ +  ++S+   +L++ G +N
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMN 250


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,318,598
Number of Sequences: 5004
Number of extensions: 65233
Number of successful extensions: 204
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)