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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_D04
         (904 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot...    31   1.0  
At1g62250.2 68414.m07023 expressed protein                             30   1.8  
At1g62250.1 68414.m07022 expressed protein                             30   1.8  
At5g09400.1 68418.m01089 potassium transporter family protein si...    29   4.2  
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si...    29   4.2  
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT...    29   5.6  
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A...    29   5.6  
At1g64150.1 68414.m07267 expressed protein contains Pfam profile...    29   5.6  
At1g47310.1 68414.m05238 expressed protein                             28   7.4  
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se...    28   9.7  
At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro...    28   9.7  
At3g04160.1 68416.m00440 expressed protein ; expression supporte...    28   9.7  
At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain...    28   9.7  

>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
           / VPS11 family protein similar to Vacuolar protein
           sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
           sapiens]; similar to Vacuolar biogenesis protein END1
           (PEP5 protein) (Vacuolar protein sorting 11)
           (Swiss-Prot:P12868) [Saccharomyces cerevisiae]
          Length = 932

 Score = 31.1 bits (67), Expect = 1.0
 Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
 Frame = +3

Query: 210 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 383
           +G Y+ A+   S     + G  I++  K LIE+  + T+D   +L T+ G    +  S  
Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557

Query: 384 --PIQFRVIFTEQTVKLINKRDHHA 452
             P+ F  +F +    L++  + +A
Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582


>At1g62250.2 68414.m07023 expressed protein
          Length = 223

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 12/43 (27%), Positives = 23/43 (53%)
 Frame = +1

Query: 106 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 234
           LC P  +  +RW  TP +  E+++ WR  C  +++    R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222


>At1g62250.1 68414.m07022 expressed protein
          Length = 267

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 12/43 (27%), Positives = 23/43 (53%)
 Frame = +1

Query: 106 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 234
           LC P  +  +RW  TP +  E+++ WR  C  +++    R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222


>At5g09400.1 68418.m01089 potassium transporter family protein
           similar to K+ transporter HAK5 [Arabidopsis thaliana]
           GI:7108597; contains Pfam profile PF02705: K+ potassium
           transporter; KUP/HAK/KT Transporter family member,
           PMID:11500563; Note: possible sequencing error causes a
           frameshift in the 4th exon|15810448|gb|AY056263
          Length = 858

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 19/73 (26%), Positives = 35/73 (47%)
 Frame = -1

Query: 292 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 113
           F +S+  S F+   +  ++A  ++   MTT  +SC    S+ LG    L+   HT+R   
Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468

Query: 112 NKVSLILAQWLSL 74
            ++ + +  W  L
Sbjct: 469 GQIYIPVLNWFLL 481


>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
           similarity to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501
          Length = 545

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
 Frame = +3

Query: 369 VKSYFPIQFRVIFTEQTVKLINKR-DHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTP 545
           V S + +QF V  +   +  IN R D HAL ++ + + +K+ F D    +    +  F P
Sbjct: 76  VPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLP 135

Query: 546 VLENNRV 566
             E  R+
Sbjct: 136 KDERPRL 142


>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
           (ATHIM) identical to SP|P34881 DNA
           (cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
           {Arabidopsis thaliana}
          Length = 1534

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = -2

Query: 354 PLSITGRRSPWCSSCRFRSDASR 286
           PLS  GR S +C+SC+ R D  +
Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891


>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
           Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
           thaliana]; similar to cytochrome P450LXXIA1, Persea
           americana, M32885
          Length = 490

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = -3

Query: 452 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 360
           G  +P +  +D + G+DH   + + RFD+FL
Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251


>At1g64150.1 68414.m07267 expressed protein contains Pfam profile
           PF01169: Uncharacterized protein family UPF0016
          Length = 370

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = -3

Query: 671 GVSGAITVDDTVITRTFRVIELQVLFVLGGHDLEVNSV 558
           G  G +T+   V+ RTF  ++  + F  GG DL ++ +
Sbjct: 197 GALGIMTIISVVLGRTFHYVDEVLPFRFGGTDLPIDDI 234


>At1g47310.1 68414.m05238 expressed protein
          Length = 395

 Score = 28.3 bits (60), Expect = 7.4
 Identities = 19/67 (28%), Positives = 35/67 (52%)
 Frame = +3

Query: 408 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 587
           T QTV  +  +   A  ++  Q   + + G ++   SKKV+W+  P +E  RV+F++ + 
Sbjct: 286 TRQTVSSVTAK-LKASGMVRFQLEIERSIGKNESVISKKVAWRTKPKIE--RVWFEVTAK 342

Query: 588 EDKQYLK 608
            +   LK
Sbjct: 343 IEGDKLK 349


>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
           several O-methyltransferases - different species;
           contains Pfam 01596 O-methyltransferase domain
          Length = 278

 Score = 27.9 bits (59), Expect = 9.7
 Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%)
 Frame = +3

Query: 177 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 356
           V  E+   S+ + +    +A  S  +  K+G +  E++K +I+NG+  + DFA+      
Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202

Query: 357 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 530
            K + + YF +  +++     + + N   H  +   +++      I   + K    K+VS
Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262

Query: 531 WKFTPV 548
               P+
Sbjct: 263 ISMVPI 268


>At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family
           protein contains Pfam profile PF00179:
           Ubiquitin-conjugating enzyme
          Length = 362

 Score = 27.9 bits (59), Expect = 9.7
 Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
 Frame = +3

Query: 498 DSKDKTSKKVSWKFTPVLENNRVYF-KIMSTEDKQYLKLDNTKGSSDDRIIYGDSTAD 668
           D +++  KK+ WK     E N+VY  +I++++ K  L+    K  +     YG S+++
Sbjct: 277 DMEEERDKKMFWKMYFAFEGNKVYCEQILNSDLKAELEKLKEKEYTLSDYYYGSSSSN 334


>At3g04160.1 68416.m00440 expressed protein ; expression supported
           by MPSS
          Length = 712

 Score = 27.9 bits (59), Expect = 9.7
 Identities = 11/37 (29%), Positives = 18/37 (48%)
 Frame = +2

Query: 284 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 394
           SR      ++E   G  +P  DK WKG  +  + +P+
Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599


>At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1)
           domain-containing protein / tetratricopeptide repeat
           (TPR)-containing protein low similarity to SP|Q99614
           Tetratricopeptide repeat protein 1 {Homo sapiens};
           contains Pfam profiles PF00564: PB1 domain, PF00515: TPR
           Domain
          Length = 697

 Score = 27.9 bits (59), Expect = 9.7
 Identities = 17/49 (34%), Positives = 25/49 (51%)
 Frame = +3

Query: 195 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 341
           YM + +GEY  AI +C+  L E      K  +KR       N +DFA++
Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,064,607
Number of Sequences: 28952
Number of extensions: 405268
Number of successful extensions: 1349
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1306
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1348
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2129873112
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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