Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_C12
(906 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.30
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.69
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 29 1.2
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 2.1
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 27 2.8
SPAC11D3.18c |||nicotinic acid plasma membrane transporter |Schi... 27 4.8
SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 26 6.4
SPACUNK4.11c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.4
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 8.5
SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 26 8.5
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.7 bits (66), Expect = 0.30
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 673
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVP 360
Query: 674 SNTTGT 691
+T +
Sbjct: 361 PTSTSS 366
Score = 30.7 bits (66), Expect = 0.30
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 673
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVP 414
Query: 674 SNTTGT 691
++ T
Sbjct: 415 PTSSST 420
Score = 29.9 bits (64), Expect = 0.52
Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +2
Query: 503 PKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSN 679
P T + S P P T+ TS S PP NST + +T V + SST P P++
Sbjct: 264 PPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNST-TPVTPTVPPTSTSSTSTPP-PPAS 321
Query: 680 TTGT 691
T+ T
Sbjct: 322 TSST 325
Score = 29.5 bits (63), Expect = 0.69
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 673
+TP P S S+P P T+ + S P T ST + + + S+T + P P
Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306
Query: 674 SNTTGT 691
+T +
Sbjct: 307 PTSTSS 312
Score = 29.1 bits (62), Expect = 0.91
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 673
+TP P S S+P P T+ + S P T ST S T + SS++ P+TP
Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249
Query: 674 S 676
+
Sbjct: 250 T 250
Score = 28.3 bits (60), Expect = 1.6
Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = +2
Query: 488 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP 664
L++ TP T P S S W TT T S T+ST +T T ++ +
Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657
Query: 665 LTPSNTTGT 691
T S++T T
Sbjct: 658 STSSSSTAT 666
>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 29.5 bits (63), Expect = 0.69
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -1
Query: 579 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 478
DLE N +V Q+ G G+FL G FG + FV+
Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444
>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 711 FVHGGLKVPVVFEGVSGAITVDDTVIT 631
F H + V G+ GAIT+DD++IT
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIIT 133
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 27.9 bits (59), Expect = 2.1
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 189 QLYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 305
Q+++++V+ Y A+ EYL+EK I + K ++E
Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 27.5 bits (58), Expect = 2.8
Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 4/68 (5%)
Frame = +1
Query: 685 WYLEPSMYE--SDVMFFVYNREYNSVMTLDEDMAANEDVKP--WGTAEKXPVIPNFLHGT 852
+Y EP+ + D + YNR Y V++LD+ + +E + W TA K P + FL
Sbjct: 155 YYQEPAFEKLLPDPLPEPYNRPYTLVLSLDDLLIHSEWTRQHGWRTA-KRPGLDYFLGYL 213
Query: 853 SSLLSVVV 876
S VV+
Sbjct: 214 SMYYEVVI 221
>SPAC11D3.18c |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 26.6 bits (56), Expect = 4.8
Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 2/75 (2%)
Frame = +1
Query: 667 DTFKHHWYLEPSMYESDVMFFVYNREYNSVMTLD--EDMAANEDVKPWGTAEKXPVIPNF 840
D ++ W+L E ++ N Y+ + E A +D K + +A
Sbjct: 243 DKMENAWFLTREEREVAIIRRDINARYHGEQHFEWSEVRKAFKDPKVYVSATSQFCADMV 302
Query: 841 LHGTSSLLSVVVKGL 885
L+G SS L V++KGL
Sbjct: 303 LYGFSSFLPVIIKGL 317
>SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 892
Score = 26.2 bits (55), Expect = 6.4
Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 6/51 (11%)
Frame = +1
Query: 631 SDDRIIYGDSTADTFKHHWYLEP----SMYESDVMFFVYNR--EYNSVMTL 765
SDDR+ GD+ + +Y++ +MYE ++ Y YN V+ L
Sbjct: 84 SDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLAL 134
>SPACUNK4.11c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 188
Score = 26.2 bits (55), Expect = 6.4
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +1
Query: 694 EPSMYESDVMFFVYNREYNSVMTLDEDMAANEDVKPWGT 810
EP++ + F ++N+E ++D++ NE+V GT
Sbjct: 74 EPNLVQGRASFGLFNKELGEENVDEKDVSKNEEVDVNGT 112
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 25.8 bits (54), Expect = 8.5
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 427 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 519
L+ K+D A K+ DQ H K+ +DK S
Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526
>SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 25.8 bits (54), Expect = 8.5
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = -3
Query: 325 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVAL 146
M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++
Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57
Query: 145 EASAHTARTKA 113
+A A T A
Sbjct: 58 NGAAKEAATAA 68
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,670,580
Number of Sequences: 5004
Number of extensions: 77232
Number of successful extensions: 298
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 266
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 296
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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