BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_C07 (888 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 55 5e-08 At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 55 5e-08 At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 49 5e-06 At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 44 1e-04 At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 44 1e-04 At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 42 7e-04 At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 38 0.007 At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 38 0.009 At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 37 0.021 At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 37 0.021 At3g01930.2 68416.m00144 nodulin family protein similar to nodul... 32 0.44 At3g01930.1 68416.m00143 nodulin family protein similar to nodul... 32 0.44 At5g14120.1 68418.m01652 nodulin family protein similar to nodul... 31 0.77 At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 31 1.4 At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 31 1.4 At2g39210.1 68415.m04816 nodulin family protein similar to nodul... 30 1.8 At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase fam... 29 5.4 At1g51805.1 68414.m05838 leucine-rich repeat protein kinase, put... 28 7.2 At4g29550.1 68417.m04214 expressed protein contains Pfam profile... 28 9.5 At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 28 9.5 At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coum... 28 9.5 >At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana} Length = 490 Score = 55.2 bits (127), Expect = 5e-08 Identities = 28/76 (36%), Positives = 42/76 (55%), Gaps = 1/76 (1%) Frame = +1 Query: 340 DVEIPNS-TLYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRD 516 D+ IPN +L+DY+++N+ + + + TG YTY +S AAN K V Sbjct: 31 DIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANF-HKLGVNQ 89 Query: 517 GDVVAVMLPNIPDFPL 564 DVV ++LPN P+F L Sbjct: 90 NDVVMLLLPNCPEFVL 105 >At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana} Length = 561 Score = 55.2 bits (127), Expect = 5e-08 Identities = 28/76 (36%), Positives = 42/76 (55%), Gaps = 1/76 (1%) Frame = +1 Query: 340 DVEIPNS-TLYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRD 516 D+ IPN +L+DY+++N+ + + + TG YTY +S AAN K V Sbjct: 31 DIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANF-HKLGVNQ 89 Query: 517 GDVVAVMLPNIPDFPL 564 DVV ++LPN P+F L Sbjct: 90 NDVVMLLLPNCPEFVL 105 >At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana} Length = 556 Score = 48.8 bits (111), Expect = 5e-06 Identities = 27/79 (34%), Positives = 40/79 (50%), Gaps = 1/79 (1%) Frame = +1 Query: 331 RYKDVEIPNST-LYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQ 507 R D+ IPN L+DY+++N+ + + + TG YTY S AA L Sbjct: 26 RLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGL-HNLG 84 Query: 508 VRDGDVVAVMLPNIPDFPL 564 V+ DVV ++LPN P+ L Sbjct: 85 VKQHDVVMILLPNSPEVVL 103 Score = 31.9 bits (69), Expect = 0.58 Identities = 16/39 (41%), Positives = 21/39 (53%) Frame = +2 Query: 590 GGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIK 706 G I TS NP +T E+ + S AK++VT V IK Sbjct: 112 GAITTSANPFFTPAEISKQAKASAAKLIVTQSRYVDKIK 150 >At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777 4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3) (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana} Length = 495 Score = 44.4 bits (100), Expect = 1e-04 Identities = 22/74 (29%), Positives = 40/74 (54%), Gaps = 1/74 (1%) Frame = +1 Query: 340 DVEIPNST-LYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRD 516 D++IPN L+ Y ++ L ++ + +TG+ YTY + + A+ L K +R Sbjct: 41 DIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL-YKLGIRK 99 Query: 517 GDVVAVMLPNIPDF 558 GDV+ ++L N +F Sbjct: 100 GDVIMILLQNSAEF 113 Score = 36.7 bits (81), Expect = 0.021 Identities = 19/55 (34%), Positives = 29/55 (52%) Frame = +2 Query: 542 QTSQIFPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIK 706 Q S F MG G + T+ NP YT+ E+ + L S AK+++T + V +K Sbjct: 108 QNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLK 162 >At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777 4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3) (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana} Length = 561 Score = 44.4 bits (100), Expect = 1e-04 Identities = 22/74 (29%), Positives = 40/74 (54%), Gaps = 1/74 (1%) Frame = +1 Query: 340 DVEIPNST-LYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRD 516 D++IPN L+ Y ++ L ++ + +TG+ YTY + + A+ L K +R Sbjct: 41 DIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL-YKLGIRK 99 Query: 517 GDVVAVMLPNIPDF 558 GDV+ ++L N +F Sbjct: 100 GDVIMILLQNSAEF 113 Score = 36.7 bits (81), Expect = 0.021 Identities = 19/55 (34%), Positives = 29/55 (52%) Frame = +2 Query: 542 QTSQIFPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIK 706 Q S F MG G + T+ NP YT+ E+ + L S AK+++T + V +K Sbjct: 108 QNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLK 162 >At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-coumaroyl-CoA synthase, putative similar to 4CL2 [gi:12229665] from Arabidopsis thaliana, 4CL1 [gi:12229631] from Nicotiana tabacum; contains Pfam AMP-binding enzyme domain PF00501; acyl-activating enzyme superfamily; identical to cDNA 4-coumarate-CoA ligase-like protein (At4g05160) GI:29893226 Length = 544 Score = 41.5 bits (93), Expect = 7e-04 Identities = 20/65 (30%), Positives = 36/65 (55%) Frame = +2 Query: 557 FPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIKEACKMAKIDL 736 FPL + + GG+ T+ NP+YT +EV + + S KI++++ + IK DL Sbjct: 92 FPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIK------GFDL 145 Query: 737 PIITI 751 P++ + Sbjct: 146 PVVLL 150 Score = 38.3 bits (85), Expect = 0.007 Identities = 20/71 (28%), Positives = 35/71 (49%) Frame = +1 Query: 352 PNSTLYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRDGDVVA 531 PN++L ++++N +P + + TG T+ Q K + A+ + +R DVV Sbjct: 25 PNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQ-LKSAVARLAHGFHRLGIRKNDVVL 83 Query: 532 VMLPNIPDFPL 564 + PN FPL Sbjct: 84 IFAPNSYQFPL 94 >At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2 [gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum Length = 570 Score = 38.3 bits (85), Expect = 0.007 Identities = 28/80 (35%), Positives = 38/80 (47%), Gaps = 5/80 (6%) Frame = +1 Query: 340 DVEIPNST-LYDYVWKNL----ERWPERTMAVCATTGRGYTYEQGFKLSNTFAANLRRKF 504 D+ IPN L DYV++ + T + TGR TY AA + R Sbjct: 35 DIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMRRIAAGIHR-L 93 Query: 505 QVRDGDVVAVMLPNIPDFPL 564 +R GDVV ++LPN P+F L Sbjct: 94 GIRHGDVVMLLLPNSPEFAL 113 Score = 31.9 bits (69), Expect = 0.58 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = +2 Query: 557 FPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVT 679 F L + + G + T+ NP YT E+ + S AK+++T Sbjct: 111 FALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASAAKMIIT 151 >At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from Oryza sativa; contains Pfam AMP-binding enzyme domain PF00501 Length = 565 Score = 37.9 bits (84), Expect = 0.009 Identities = 17/66 (25%), Positives = 36/66 (54%) Frame = +2 Query: 548 SQIFPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIKEACKMAK 727 S +FP+ ++ ++ G IIT+ NPI T+ E+ + + S + T T ++ + + Sbjct: 109 SILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSRPVLAFT---TCKLVSKLAAASN 165 Query: 728 IDLPII 745 +LP++ Sbjct: 166 FNLPVV 171 >At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to SP|P14912 and SP|P14913 from Petroselinum crispum; contains Pfam AMP-binding enzyme domain PF00501 Length = 473 Score = 36.7 bits (81), Expect = 0.021 Identities = 18/63 (28%), Positives = 33/63 (52%) Frame = +2 Query: 548 SQIFPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIKEACKMAK 727 S +FP+ + ++ G IIT+ NP+ T++E+ + + S + T + + I A K Sbjct: 92 SILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLP 151 Query: 728 IDL 736 I L Sbjct: 152 IVL 154 >At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to SP|P14912 and SP|P14913 from Petroselinum crispum; contains Pfam AMP-binding enzyme domain PF00501 Length = 546 Score = 36.7 bits (81), Expect = 0.021 Identities = 18/63 (28%), Positives = 33/63 (52%) Frame = +2 Query: 548 SQIFPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETVAIIKEACKMAK 727 S +FP+ + ++ G IIT+ NP+ T++E+ + + S + T + + I A K Sbjct: 92 SILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLP 151 Query: 728 IDL 736 I L Sbjct: 152 IVL 154 >At3g01930.2 68416.m00144 nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] GI:3329368, nodule-specific protein Nlj70 [Lotus japonicus] GI:3329366 Length = 584 Score = 32.3 bits (70), Expect = 0.44 Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 2/40 (5%) Frame = -2 Query: 506 WNFLLRFAANVLESLKPCSYVYPRPV-VAQTAIVLS-GHL 393 WNFL R L Y YPRPV +A +V+S GH+ Sbjct: 412 WNFLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHI 451 >At3g01930.1 68416.m00143 nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] GI:3329368, nodule-specific protein Nlj70 [Lotus japonicus] GI:3329366 Length = 471 Score = 32.3 bits (70), Expect = 0.44 Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 2/40 (5%) Frame = -2 Query: 506 WNFLLRFAANVLESLKPCSYVYPRPV-VAQTAIVLS-GHL 393 WNFL R L Y YPRPV +A +V+S GH+ Sbjct: 299 WNFLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHI 338 >At5g14120.1 68418.m01652 nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] GI:3329368, nodule-specific protein Nlj70 [Lotus japonicus] GI:3329366 Length = 579 Score = 31.5 bits (68), Expect = 0.77 Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 2/40 (5%) Frame = -2 Query: 506 WNFLLRFAANVLESLKPCSYVYPRPV-VAQTAIVLS-GHL 393 WNFL R L Y YPRPV +A +++S GH+ Sbjct: 407 WNFLGRIGGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHI 446 >At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to 4CL from Pinus taeda, gi:515503, gi:1143308; contains Pfam AMP-binding enzyme domain PF00501 Length = 566 Score = 30.7 bits (66), Expect = 1.4 Identities = 16/52 (30%), Positives = 28/52 (53%) Frame = +1 Query: 409 TMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRDGDVVAVMLPNIPDFPL 564 T + + TG ++ + + + AA + VR GDVV+++LPN FP+ Sbjct: 62 TALIDSLTGFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPM 113 Score = 28.3 bits (60), Expect = 7.2 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = +2 Query: 548 SQIFPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVTLPETV 694 S FP+ + ++ G I+T++NP + E+++ + + T E V Sbjct: 108 SVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVSECSVGLAFTSTENV 156 >At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to gi:112801 from Petroselinum crispum, GB:AAD40664 from [Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559 (1991)); contains Pfam AMP-binding enzyme domain PF00501 Length = 542 Score = 30.7 bits (66), Expect = 1.4 Identities = 11/41 (26%), Positives = 24/41 (58%) Frame = +2 Query: 557 FPLXTMGILEAGGIITSINPIYTAHEVQRXLVLSEAKIVVT 679 + + +GI+ AGG+ + NP E+++ + S A+ ++T Sbjct: 91 YGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGIIT 131 >At2g39210.1 68415.m04816 nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] GI:3329368, nodule-specific protein Nlj70 [Lotus japonicus] GI:3329366 Length = 601 Score = 30.3 bits (65), Expect = 1.8 Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 2/51 (3%) Frame = -2 Query: 539 SITATTSPSRTWNFLLRFAANVLESLKPCSYVYPRPVVAQTAIVLS--GHL 393 S++ S WN+ R + V+ + Y +PRP++ ++LS GHL Sbjct: 379 SVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHL 429 >At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase family protein similar to SP|Q42524 and SP|Q9S725; contains Pfam AMP-binding enzyme domain PF00501 Length = 447 Score = 28.7 bits (61), Expect = 5.4 Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 2/51 (3%) Frame = +1 Query: 400 PER--TMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRDGDVVAVMLPN 546 P+R T + A+TG T+ +++ + A L + +R GDVV ++ PN Sbjct: 48 PQRGTTAFIDASTGHRLTFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPN 98 >At1g51805.1 68414.m05838 leucine-rich repeat protein kinase, putative similar to light repressible receptor protein kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376; contains leucine rich repeat (LRR) domains, Pfam:PF00560; contains protein kinase domain, Pfam:PF00069 Length = 884 Score = 28.3 bits (60), Expect = 7.2 Identities = 17/61 (27%), Positives = 26/61 (42%) Frame = +1 Query: 298 VFGVLIM**VLRYKDVEIPNSTLYDYVWKNLERWPERTMAVCATTGRGYTYEQGFKLSNT 477 + G LI+ V R K TL Y+ + R P + T + +TY Q ++N Sbjct: 519 IIGALILFLVFRKKKASKVEGTLPSYMQASDGRSPRSSEPAIVTKNKRFTYSQVVIMTNN 578 Query: 478 F 480 F Sbjct: 579 F 579 >At4g29550.1 68417.m04214 expressed protein contains Pfam profile PF04776: Protein of unknown function (DUF626) Length = 358 Score = 27.9 bits (59), Expect = 9.5 Identities = 14/44 (31%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Frame = +1 Query: 340 DVEIPNSTLYDYVWKNLERWPERTMAVCATTG-RGYTYEQGFKL 468 D+ IP S+LY Y N + + +CA G Y +++G L Sbjct: 104 DIRIPTSSLYVYKCANNDDLRGDIVGICARVGLHWYNFQKGSNL 147 >At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, putative / steroid 5-alpha-reductase, putative identical to gi:1280611; contains a steroid 5-alpha reductase, C-terminal domain Length = 262 Score = 27.9 bits (59), Expect = 9.5 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -2 Query: 722 PSYKPPL*WPLSPAM*PRFWLRIIQXAFGLHAL 624 P+ PP+ W + + P WL ++ FG HAL Sbjct: 45 PTVSPPIAWFVMES--PTLWLTLLLFPFGRHAL 75 >At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coumaroyl-CoA synthase family similar to SP|Q42524 and SP|Q9S725; contains Pfam AMP-binding enzyme domain PF00501 Length = 412 Score = 27.9 bits (59), Expect = 9.5 Identities = 14/47 (29%), Positives = 25/47 (53%) Frame = +1 Query: 406 RTMAVCATTGRGYTYEQGFKLSNTFAANLRRKFQVRDGDVVAVMLPN 546 +T + A TG+ T+ ++ + A L + +R GDVV ++ PN Sbjct: 52 KTAFIDAATGQCLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPN 98 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,877,444 Number of Sequences: 28952 Number of extensions: 314881 Number of successful extensions: 822 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 790 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 817 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2081245872 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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