Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_B23
(1277 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g62190.1 68418.m07807 DEAD box RNA helicase (PRH75) nearly id... 36 0.057
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 35 0.13
At5g58160.1 68418.m07280 formin homology 2 domain-containing pro... 33 0.40
At1g31810.1 68414.m03904 formin homology 2 domain-containing pro... 27 2.4
At1g04800.1 68414.m00476 glycine-rich protein 30 2.8
At4g01985.1 68417.m00265 expressed protein 29 5.0
At3g11030.1 68416.m01331 expressed protein contains Pfam domain ... 29 5.0
At5g62640.1 68418.m07862 proline-rich family protein contains pr... 29 6.5
At4g38680.1 68417.m05477 cold-shock DNA-binding family protein c... 29 6.5
At2g27350.5 68415.m03295 OTU-like cysteine protease family prote... 29 6.5
At2g27350.4 68415.m03294 OTU-like cysteine protease family prote... 29 6.5
At2g27350.3 68415.m03293 OTU-like cysteine protease family prote... 29 6.5
At2g27350.2 68415.m03292 OTU-like cysteine protease family prote... 29 6.5
At2g27350.1 68415.m03291 OTU-like cysteine protease family prote... 29 6.5
At2g15880.1 68415.m01820 leucine-rich repeat family protein / ex... 29 6.5
At2g43150.1 68415.m05358 proline-rich extensin-like family prote... 29 8.7
At2g28490.1 68415.m03462 cupin family protein similar to preproM... 29 8.7
At1g26150.1 68414.m03192 protein kinase family protein similar t... 29 8.7
At3g55950.1 68416.m06217 protein kinase family protein contains ... 24 9.1
At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family... 24 9.4
At1g72790.1 68414.m08415 hydroxyproline-rich glycoprotein family... 24 9.4
>At5g62190.1 68418.m07807 DEAD box RNA helicase (PRH75) nearly
identical to RNA helicase [Arabidopsis thaliana]
GI:1488521; contains Pfam profiles PF00270: DEAD/DEAH box
helicase, PF00271: Helicase conserved C-terminal domain
Length = 671
Score = 35.9 bits (79), Expect = 0.057
Identities = 17/33 (51%), Positives = 18/33 (54%)
Frame = -3
Query: 1083 QQXFGGWXRGXRDXGXWGXVXGXGGGXGXGGXG 985
Q+ FGG RG R G G G GGG G GG G
Sbjct: 633 QKRFGGGGRGNRFGGGGGNRFGGGGGRGRGGSG 665
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 34.7 bits (76), Expect = 0.13
Identities = 16/40 (40%), Positives = 17/40 (42%)
Frame = +2
Query: 902 LYSPXLPXRXRXPTAPXXXAPIXXGXTSPXPPXPXPPPXP 1021
+YSP P P P AP T P PP P PP P
Sbjct: 509 VYSPPPPPVYSSPPPPPSPAPTPVYCTRPPPPPPHSPPPP 548
Score = 29.9 bits (64), Expect = 3.7
Identities = 14/37 (37%), Positives = 14/37 (37%)
Frame = +2
Query: 911 PXLPXRXRXPTAPXXXAPIXXGXTSPXPPXPXPPPXP 1021
P P P P P SP PP P PPP P
Sbjct: 457 PPPPPPVYSPPPPPPPPPPPPPVYSPPPPSPPPPPPP 493
Score = 29.1 bits (62), Expect = 6.5
Identities = 15/40 (37%), Positives = 16/40 (40%)
Frame = +2
Query: 902 LYSPXLPXRXRXPTAPXXXAPIXXGXTSPXPPXPXPPPXP 1021
L SP P +P P SP PP P PPP P
Sbjct: 423 LTSPPPPSPPPPVYSPPPPPPPPPPVYSPPPPPPPPPPPP 462
Score = 28.7 bits (61), Expect = 8.7
Identities = 13/37 (35%), Positives = 14/37 (37%)
Frame = +2
Query: 911 PXLPXRXRXPTAPXXXAPIXXGXTSPXPPXPXPPPXP 1021
P P P P P + P PP P PPP P
Sbjct: 442 PPPPPPVYSPPPPPPPPPPPPVYSPPPPPPPPPPPPP 478
>At5g58160.1 68418.m07280 formin homology 2 domain-containing protein
/ FH2 domain-containing protein low similarity to
SP|Q05858 Formin (Limb deformity protein) {Gallus
gallus}; contains Pfam profile PF02181: Formin Homology
2(FH2) Domain
Length = 1307
Score = 33.1 bits (72), Expect = 0.40
Identities = 13/30 (43%), Positives = 14/30 (46%)
Frame = +2
Query: 938 PTAPXXXAPIXXGXTSPXPPXPXPPPXPXT 1027
P P PI + P PP P PPP P T
Sbjct: 712 PAPPAPPTPIVHTSSPPPPPPPPPPPAPPT 741
>At1g31810.1 68414.m03904 formin homology 2 domain-containing protein
/ FH2 domain-containing protein low similarity to
SP|P48608 Diaphanous protein {Drosophila melanogaster};
contains Pfam profile PF02181: Formin Homology 2(FH2)
Domain
Length = 1201
Score = 27.5 bits (58), Expect(2) = 2.4
Identities = 11/28 (39%), Positives = 11/28 (39%)
Frame = +2
Query: 938 PTAPXXXAPIXXGXTSPXPPXPXPPPXP 1021
P P I P PP P PPP P
Sbjct: 578 PPPPLPSRSIPPPLAQPPPPRPPPPPPP 605
Score = 24.6 bits (51), Expect(2) = 6.8
Identities = 10/31 (32%), Positives = 13/31 (41%)
Frame = +2
Query: 920 PXRXRXPTAPXXXAPIXXGXTSPXPPXPXPP 1012
P R P P + ++P PP P PP
Sbjct: 596 PPRPPPPPPPPPSSRSIPSPSAPPPPPPPPP 626
Score = 22.6 bits (46), Expect(2) = 6.8
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 986 PXPPXPXPPP 1015
P PP P PPP
Sbjct: 639 PPPPPPPPPP 648
Score = 21.4 bits (43), Expect(2) = 2.4
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +2
Query: 995 PXPXPPPXPXT 1027
P P PPP P T
Sbjct: 639 PPPPPPPPPPT 649
>At1g04800.1 68414.m00476 glycine-rich protein
Length = 200
Score = 30.3 bits (65), Expect = 2.8
Identities = 25/88 (28%), Positives = 33/88 (37%)
Frame = -3
Query: 1071 GGWXRGXRDXGXWGXVXGXGGGXGXGGXGEVXPXXIGAXXXGAVGXRXR*GXXGE*RRXN 892
GGW G G G GGG GG G+ G G G + G G+
Sbjct: 72 GGWIGGSVGGFGGGIGGGFGGGGFGGGAGKGVDGGFGKGVDGGAG-KGVDGGAGK-GFDG 129
Query: 891 GLGELLXXGXGXWRXREVXXQVPGGVGR 808
G+G+ + G G V GG+G+
Sbjct: 130 GVGKGVDGGAGKGFDGGVGKGFEGGIGK 157
>At4g01985.1 68417.m00265 expressed protein
Length = 579
Score = 29.5 bits (63), Expect = 5.0
Identities = 30/87 (34%), Positives = 33/87 (37%)
Frame = -3
Query: 1071 GGWXRGXRDXGXWGXVXGXGGGXGXGGXGEVXPXXIGAXXXGAVGXRXR*GXXGE*RRXN 892
GG G G G V G GG G GG G +GA G VG G G
Sbjct: 445 GGAVGGAVGGGGGGSVGG--GGRGSGGAGGGTGGSVGA--GGGVGVGGGGGIGG--GAGG 498
Query: 891 GLGELLXXGXGXWRXREVXXQVPGGVG 811
G+G + G G V V GGVG
Sbjct: 499 GVGGGVGGGVGGGVRGAVGGAVGGGVG 525
Score = 29.1 bits (62), Expect = 6.5
Identities = 20/54 (37%), Positives = 21/54 (38%)
Frame = -3
Query: 1071 GGWXRGXRDXGXWGXVXGXGGGXGXGGXGEVXPXXIGAXXXGAVGXRXR*GXXG 910
GG G G G G GGG G GG G +G GAVG G G
Sbjct: 406 GGASGGVGGAGGAGGSVGAGGGVG-GGVGGGVGGGVGGAVGGAVGGAVGGGGGG 458
Score = 28.7 bits (61), Expect = 8.7
Identities = 19/54 (35%), Positives = 21/54 (38%), Gaps = 1/54 (1%)
Frame = -3
Query: 1068 GWXRGXRDXGXWGXVXGXGGGXGXGGXGEVXPXX-IGAXXXGAVGXRXR*GXXG 910
G G G G G GGG G GG G V +G GA+G G G
Sbjct: 50 GAGAGGGASGGIGVGGGGGGGGGIGGSGGVGAGGGVGGGAGGAIGGGASGGAGG 103
>At3g11030.1 68416.m01331 expressed protein contains Pfam domain
PF03005: Arabidopsis proteins of unknown function
Length = 451
Score = 29.5 bits (63), Expect = 5.0
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +2
Query: 938 PTAPXXXAPIXXGXTSPXPPXPXPPP 1015
PT+P +P P PP P PPP
Sbjct: 69 PTSPPPPSPPPPSPPPPSPPPPSPPP 94
>At5g62640.1 68418.m07862 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 520
Score = 29.1 bits (62), Expect = 6.5
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 938 PTAPXXXAPIXXGXTSPXPPXPXPPPXP 1021
P P P G T P P P PPP P
Sbjct: 198 PLPPLPPLPPTTGLTLPHSPFPPPPPGP 225
>At4g38680.1 68417.m05477 cold-shock DNA-binding family protein
contains Pfam domains PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 203
Score = 29.1 bits (62), Expect = 6.5
Identities = 13/29 (44%), Positives = 14/29 (48%)
Frame = -3
Query: 1071 GGWXRGXRDXGXWGXVXGXGGGXGXGGXG 985
GG+ G G G G GGG G GG G
Sbjct: 149 GGYGGGGGGYGGGGGYGGGGGGYGGGGRG 177
>At2g27350.5 68415.m03295 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine protease
Length = 388
Score = 29.1 bits (62), Expect = 6.5
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +2
Query: 959 APIXXGXTSPXPPXPXPPPXP 1021
+P+ G S PP P PPP P
Sbjct: 104 SPVSGGSNSDSPPVPAPPPKP 124
>At2g27350.4 68415.m03294 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine protease
Length = 388
Score = 29.1 bits (62), Expect = 6.5
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +2
Query: 959 APIXXGXTSPXPPXPXPPPXP 1021
+P+ G S PP P PPP P
Sbjct: 104 SPVSGGSNSDSPPVPAPPPKP 124
>At2g27350.3 68415.m03293 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine protease
Length = 506
Score = 29.1 bits (62), Expect = 6.5
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +2
Query: 959 APIXXGXTSPXPPXPXPPPXP 1021
+P+ G S PP P PPP P
Sbjct: 104 SPVSGGSNSDSPPVPAPPPKP 124
>At2g27350.2 68415.m03292 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine protease
Length = 505
Score = 29.1 bits (62), Expect = 6.5
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +2
Query: 959 APIXXGXTSPXPPXPXPPPXP 1021
+P+ G S PP P PPP P
Sbjct: 104 SPVSGGSNSDSPPVPAPPPKP 124
>At2g27350.1 68415.m03291 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine protease
Length = 505
Score = 29.1 bits (62), Expect = 6.5
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +2
Query: 959 APIXXGXTSPXPPXPXPPPXP 1021
+P+ G S PP P PPP P
Sbjct: 104 SPVSGGSNSDSPPVPAPPPKP 124
>At2g15880.1 68415.m01820 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 727
Score = 29.1 bits (62), Expect = 6.5
Identities = 27/114 (23%), Positives = 30/114 (26%), Gaps = 4/114 (3%)
Frame = +1
Query: 922 SXAXPTHRTXPXGSYXPWXYFTXPXAPXSXPXPXHXSPXXXISXSTXPTSEPLLPX*XSX 1101
S P H P P + P S P P H P S P P P
Sbjct: 544 SPPPPVHSPPPPVHSPPPPVHSPPPPVHSPPPPVHSPPPPVYSPPPPPVHSPPPPVHSPP 603
Query: 1102 XSVHXXXXXXXXTXVXXQISVXPPS--APXXXSHHPXLP--XPTKXXYXLXPXP 1251
VH + PP +P H P P P Y P P
Sbjct: 604 PPVHSPPPPVYSPPPPPPVHSPPPPVFSPPPPVHSPPPPVYSPPPPVYSPPPPP 657
>At2g43150.1 68415.m05358 proline-rich extensin-like family protein
similar to CRANTZ hydroxyproline-rich glycoprotein
[Manihot esculenta] gi|7211797|gb|AAF40442; similar to
extensin gi|1165322|gb|AAB53156; contains proline-rich
extensin domains, INTERPRO:IPR002965
Length = 212
Score = 28.7 bits (61), Expect = 8.7
Identities = 13/36 (36%), Positives = 14/36 (38%)
Frame = +2
Query: 908 SPXLPXRXRXPTAPXXXAPIXXGXTSPXPPXPXPPP 1015
SP P + P P P SP PP PPP
Sbjct: 35 SPPPPYEYKSPPPPVKSPPPPYEYKSPPPPVKSPPP 70
>At2g28490.1 68415.m03462 cupin family protein similar to
preproMP27-MP32 [Cucurbita cv. Kurokawa Amakuri]
GI:691752, allergen Gly m Bd 28K [Glycine max]
GI:12697782, vicilin [Matteuccia struthiopteris]
GI:1019792; contains Pfam profile PF00190: Cupin
Length = 511
Score = 28.7 bits (61), Expect = 8.7
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = -3
Query: 1071 GGWXRGXRDXGXWGXVXGXGGGXGXGGXG 985
G W G G WG GG G GG G
Sbjct: 49 GAWGGGGGGGGAWGGEGEGGGEWGGGGEG 77
>At1g26150.1 68414.m03192 protein kinase family protein similar to Pto
kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 28.7 bits (61), Expect = 8.7
Identities = 13/32 (40%), Positives = 13/32 (40%)
Frame = +2
Query: 920 PXRXRXPTAPXXXAPIXXGXTSPXPPXPXPPP 1015
P P P AP TSP PP PPP
Sbjct: 119 PPESSPPPPPPTEAPPTTPITSPSPPTNPPPP 150
>At3g55950.1 68416.m06217 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
cytokinin-regulated kinase 1 [Nicotiana tabacum]
gi|10998537|gb|AAG25966
Length = 814
Score = 24.2 bits (50), Expect(2) = 9.1
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = +2
Query: 992 PPXPXPPPXPXT 1027
PP P PPP P T
Sbjct: 372 PPPPPPPPSPST 383
Score = 22.6 bits (46), Expect(2) = 9.1
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 986 PXPPXPXPPP 1015
P PP P PPP
Sbjct: 369 PLPPPPPPPP 378
>At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family
protein Common family members: At5g26070, At5g19800,
At1g72790 [Arabidopsis thaliana]
Length = 575
Score = 24.2 bits (50), Expect(2) = 9.4
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = +2
Query: 983 SPXPPXPXPPP 1015
SP PP P PPP
Sbjct: 384 SPPPPPPPPPP 394
Score = 22.6 bits (46), Expect(2) = 9.4
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 992 PPXPXPPPXP 1021
PP P PPP P
Sbjct: 386 PPPPPPPPPP 395
>At1g72790.1 68414.m08415 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 561
Score = 24.2 bits (50), Expect(2) = 9.4
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = +2
Query: 983 SPXPPXPXPPP 1015
SP PP P PPP
Sbjct: 364 SPPPPPPPPPP 374
Score = 22.6 bits (46), Expect(2) = 9.4
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 992 PPXPXPPPXP 1021
PP P PPP P
Sbjct: 394 PPPPPPPPPP 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,405,687
Number of Sequences: 28952
Number of extensions: 133960
Number of successful extensions: 1266
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 401
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 977
length of database: 12,070,560
effective HSP length: 83
effective length of database: 9,667,544
effective search space used: 3306300048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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