Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_B16
(910 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 58 6e-09
At2g35580.1 68415.m04357 serpin family protein / serine protease... 51 1e-06
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 49 5e-06
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 48 7e-06
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 48 9e-06
At1g64030.1 68414.m07252 serpin family protein / serine protease... 44 1e-04
At2g14540.1 68415.m01628 serpin family protein / serine protease... 38 0.009
At1g64010.1 68414.m07250 serpin, putative / serine protease inhi... 33 0.26
At1g62170.1 68414.m07013 serpin family protein / serine protease... 33 0.26
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 31 1.1
At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 29 5.6
At5g53730.1 68418.m06677 harpin-induced family protein / HIN1 fa... 28 7.4
At3g60740.1 68416.m06795 tubulin folding cofactor D identical to... 28 9.8
At2g37960.1 68415.m04659 expressed protein 28 9.8
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 58.4 bits (135), Expect = 6e-09
Identities = 39/157 (24%), Positives = 72/157 (45%), Gaps = 7/157 (4%)
Frame = +1
Query: 205 NLADDKNVIASPLGVMLLLSLYESGAGAQSKEEIREILGGGEAQESSHTYGLLNQRYAEF 384
++A+ NV+ SP+ + +LLSL +G+ +KEEI L + + E
Sbjct: 25 DVANGSNVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTER 84
Query: 385 DPKFLTVANKIYVSDQYKLADAFSR-TANLFRSEVDNINFSA-PKNAADIINRWADEQTQ 558
L+ A+ +++ L +F N +++ ++F+ P D +N WAD T
Sbjct: 85 SDLCLSTAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFATKPVEVIDEVNIWADVHTN 144
Query: 559 GHIKTPVSEDKIDPA-----TAVAMFNVIFFQGALAR 654
G IK +S D D + + + N ++F+ A +R
Sbjct: 145 GLIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSR 181
Score = 48.4 bits (110), Expect = 7e-06
Identities = 30/86 (34%), Positives = 46/86 (53%), Gaps = 3/86 (3%)
Frame = +2
Query: 647 WHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKEP--GFRM 820
W F+A T++ DFH+ + T+K P M + + + G +++ LPY E F M
Sbjct: 179 WSRKFDAKLTKDNDFHLLDGNTVKVPFMMSYKDQYLRGYD--GFQVLRLPYVEDKRHFSM 236
Query: 821 VVVLPDXIDGLPAXLEKXA-EXGLLE 895
+ LP+ DGL A LEK + E G L+
Sbjct: 237 YIYLPNDKDGLAALLEKISTEPGFLD 262
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 50.8 bits (116), Expect = 1e-06
Identities = 35/92 (38%), Positives = 48/92 (52%), Gaps = 6/92 (6%)
Frame = +2
Query: 641 GHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYK-----E 805
G W F+ S T++ DFH+ + T ++ P M S YT E G K+I L Y+
Sbjct: 176 GRWDSQFDPSLTKDSDFHLLDGTKVRVPFM-TGASCRYTHVYE-GFKVINLQYRRGREDS 233
Query: 806 PGFRMVVVLPDXIDGLPAXLEKXAE-XGLLED 898
F M + LPD DGLP+ LE+ A G L+D
Sbjct: 234 RSFSMQIYLPDEKDGLPSMLERLASTRGFLKD 265
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 48.8 bits (111), Expect = 5e-06
Identities = 30/80 (37%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = +2
Query: 638 RGHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKEP--G 811
+G W F+A T+ DFH+ + T +K P M + Y E + G K++ LPY E
Sbjct: 176 KGAWSKKFDAKLTKSYDFHLLDGTMVKVPFMTNYKKQ-YLEYYD-GFKVLRLPYVEDQRQ 233
Query: 812 FRMVVVLPDXIDGLPAXLEK 871
F M + LP+ DGLP LE+
Sbjct: 234 FAMYIYLPNDRDGLPTLLEE 253
Score = 46.4 bits (105), Expect = 3e-05
Identities = 34/157 (21%), Positives = 74/157 (47%), Gaps = 8/157 (5%)
Frame = +1
Query: 208 LADDKNVIASPLGVMLLLSLYESGAGAQSKEEIRE--ILGGGEAQES--SHTYGLLNQRY 375
+A+ N++ SP+ + +LL L +G+ +KE+I +L + + + T +
Sbjct: 25 VANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALNDG 84
Query: 376 AEFDPKFLTVANKIYVSDQYKLADAF-SRTANLFRSEVDNINFSA-PKNAADIINRWADE 549
E L+ A +++ +F N + + + ++F+ P + +N WA+
Sbjct: 85 MERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFATKPAEVINEVNAWAEV 144
Query: 550 QTQGHIKTPVSEDKIDP--ATAVAMFNVIFFQGALAR 654
T G IK +S+D I + + + N ++F+GA ++
Sbjct: 145 HTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSK 181
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 48.4 bits (110), Expect = 7e-06
Identities = 27/80 (33%), Positives = 44/80 (55%), Gaps = 2/80 (2%)
Frame = +2
Query: 638 RGHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKEP--G 811
+G W F+A+ T++ DFH+ + T++K P M + + + G K++ LPY E
Sbjct: 111 KGAWSSKFDANMTKKNDFHLLDGTSVKVPFMTNYEDQYLRSYD--GFKVLRLPYIEDQRQ 168
Query: 812 FRMVVVLPDXIDGLPAXLEK 871
F M + LP+ +GL LEK
Sbjct: 169 FSMYIYLPNDKEGLAPLLEK 188
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 48.0 bits (109), Expect = 9e-06
Identities = 36/154 (23%), Positives = 72/154 (46%), Gaps = 9/154 (5%)
Frame = +1
Query: 208 LADDKNVIASPLGVMLLLSLYESGAGAQSKEEIREILGGGEAQE-----SSHTYGLLNQR 372
++ + NVI SP + ++LS+ +G+ +K++I L + S +L
Sbjct: 25 VSQNSNVIFSPASINVVLSIIAAGSAGATKDQILSFLKFSSTDQLNSFSSEIVSAVLADG 84
Query: 373 YAEFDPKFLTVANKIYVSDQYKLADAFSRTA-NLFRSEVDNINFSAPKNAADII---NRW 540
A PK L+VAN ++ +F + + +++ + +F + A ++I N W
Sbjct: 85 SANGGPK-LSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQS--KAVEVIAEVNSW 141
Query: 541 ADEQTQGHIKTPVSEDKIDPATAVAMFNVIFFQG 642
A+++T G I + E D T + N ++F+G
Sbjct: 142 AEKETNGLITEVLPEGSADSMTKLIFANALYFKG 175
Score = 37.5 bits (83), Expect = 0.012
Identities = 24/82 (29%), Positives = 41/82 (50%), Gaps = 4/82 (4%)
Frame = +2
Query: 638 RGHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPY----KE 805
+G W+ F+ S T+E +FH+ + + P M + + + + G K++ LPY +
Sbjct: 174 KGTWNEKFDESLTQEGEFHLLDGNKVTAPFMTSKKKQYVSAYD--GFKVLGLPYLQGQDK 231
Query: 806 PGFRMVVVLPDXIDGLPAXLEK 871
F M LPD +GL L+K
Sbjct: 232 RQFSMYFYLPDANNGLSDLLDK 253
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 44.0 bits (99), Expect = 1e-04
Identities = 28/90 (31%), Positives = 42/90 (46%), Gaps = 6/90 (6%)
Frame = +2
Query: 626 SFSSRGHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKE 805
+ S +G W PF T + DF++ T++ P M ++ + + G K++ LPY+
Sbjct: 171 ALSFKGAWKRPFEKYYTRDNDFYLVNGTSVSVPFMSSYENQYVRAYD--GFKVLRLPYQR 228
Query: 806 PG------FRMVVVLPDXIDGLPAXLEKXA 877
F M LPD DGL LEK A
Sbjct: 229 GSDDTNRKFSMYFYLPDKKDGLDDLLEKMA 258
Score = 35.9 bits (79), Expect = 0.037
Identities = 35/154 (22%), Positives = 59/154 (38%), Gaps = 8/154 (5%)
Frame = +1
Query: 217 DKNVIASPLGVMLLLSLYESGAGAQSKE-EIREILGGGEAQESSHTYGLLNQ-----RYA 378
D NVI SP + ++++ +G G +I L E + L R A
Sbjct: 28 DSNVIFSPASINSAITMHAAGPGGDLVSGQILSFLRSSSIDELKTVFRELASVVYADRSA 87
Query: 379 EFDPKFLTVANKIYVSDQYKLADAFSRT-ANLFRSEVDNINF-SAPKNAADIINRWADEQ 552
PK +T AN +++ F N F++ ++F S + +N W +
Sbjct: 88 TGGPK-ITAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWVEHH 146
Query: 553 TQGHIKTPVSEDKIDPATAVAMFNVIFFQGALAR 654
T IK + + + T N + F+GA R
Sbjct: 147 TNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKR 180
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 37.9 bits (84), Expect = 0.009
Identities = 25/84 (29%), Positives = 39/84 (46%), Gaps = 6/84 (7%)
Frame = +2
Query: 638 RGHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKE---- 805
+G W F+ S T +K FH+ ++ P MR + F + G K++ LPY++
Sbjct: 203 KGAWEKAFDKSMTRDKPFHLLNGKSVSVPFMRSYEKQFIEAYD--GFKVLRLPYRQGRDD 260
Query: 806 --PGFRMVVVLPDXIDGLPAXLEK 871
F M + LPD L LE+
Sbjct: 261 TNREFSMYLYLPDKKGELDNLLER 284
>At1g64010.1 68414.m07250 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 185
Score = 33.1 bits (72), Expect = 0.26
Identities = 21/73 (28%), Positives = 37/73 (50%), Gaps = 5/73 (6%)
Frame = +2
Query: 674 TEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKEPG-----FRMVVVLPD 838
T+++DFH+ T++ M + + + G K+++LP+++ F M LPD
Sbjct: 2 TKDRDFHLINGTSVSVSLMSSYKDQYIEAYD--GFKVLKLPFRQGNDTSRNFSMHFYLPD 59
Query: 839 XIDGLPAXLEKXA 877
DGL +EK A
Sbjct: 60 EKDGLDNLVEKMA 72
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 33.1 bits (72), Expect = 0.26
Identities = 32/158 (20%), Positives = 64/158 (40%), Gaps = 12/158 (7%)
Frame = +1
Query: 205 NLADDKNVIASPLGVMLLLSLYESGAGAQSKEEIREILGGGEAQESSHTYGLLNQRYAEF 384
++A + N + SP + L++ + +G + EE+R + S+ + + A
Sbjct: 85 SVAKNSNFVFSPASINAALTMVAASSGGEQGEELRSFILSFLKSSSTDELNAIFREIASV 144
Query: 385 ---------DPKFLTVANKIYVSDQYKLADAFSRTA--NLFRSEVDNINF-SAPKNAADI 528
PK + V N +++ DQ + S+ N F + ++F S +
Sbjct: 145 VLVDGSKKGGPK-IAVVNGMWM-DQSLSVNPLSKDLFKNFFSAAFAQVDFRSKAEEVRTE 202
Query: 529 INRWADEQTQGHIKTPVSEDKIDPATAVAMFNVIFFQG 642
+N WA T G IK + + T + ++F+G
Sbjct: 203 VNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKG 240
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 31.1 bits (67), Expect = 1.1
Identities = 16/56 (28%), Positives = 31/56 (55%)
Frame = +2
Query: 638 RGHWHVPFNASETEEKDFHVDEKTTIKKPTMRLLQSLFYTENEELGAKMIELPYKE 805
+G W F+ S T++ +FH ++ + P MR +S + + G K++ LPY++
Sbjct: 52 KGAWENKFDKSSTKDNEFHQGKEVHV--PFMRSYESQYIMACD--GFKVLGLPYQQ 103
>At3g60860.1 68416.m06808 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor, Homo
sapiens, GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1793
Score = 28.7 bits (61), Expect = 5.6
Identities = 18/51 (35%), Positives = 24/51 (47%), Gaps = 4/51 (7%)
Frame = +1
Query: 283 GAQSKEEIREILGG----GEAQESSHTYGLLNQRYAEFDPKFLTVANKIYV 423
G + E IR L G GEAQ+ +RY + +PK T A+ YV
Sbjct: 693 GMEFDEAIRTFLEGFRLPGEAQKIDRIMEKFAERYCKCNPKVFTSADSAYV 743
>At5g53730.1 68418.m06677 harpin-induced family protein / HIN1
family protein / harpin-responsive family protein
similar to harpin-induced protein hin1 ( GI:1619321)
[Nicotiana tabacum];
Length = 213
Score = 28.3 bits (60), Expect = 7.4
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 621 KHCNGRGGVNFVFAHRSLYVSLSLF 547
KHC +GG+N H+ L+ + S F
Sbjct: 10 KHCAKKGGININNRHKKLFFTFSTF 34
>At3g60740.1 68416.m06795 tubulin folding cofactor D identical to
tubulin folding cofactor D GI:20514263 from [Arabidopsis
thaliana]
Length = 1254
Score = 27.9 bits (59), Expect = 9.8
Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 3/67 (4%)
Frame = -1
Query: 325 LPQGFPLSLPWTVHRLQTRTRK---AAASRREETLSHSCRQLGCRIP*ESXKEVLSMASP 155
LP+ FPL +P V L R+ + S + ++ C G + K VL +P
Sbjct: 470 LPRSFPLVVPVIVKALHYDVRRGPHSVGSHVRDAAAYVCWAFGRAYSHKDMKNVLDQLAP 529
Query: 154 SLVLENS 134
L++ S
Sbjct: 530 DLLIVGS 536
>At2g37960.1 68415.m04659 expressed protein
Length = 480
Score = 27.9 bits (59), Expect = 9.8
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +1
Query: 418 YVSDQYKLADAFSRTANLFRSEVDNINFSAP 510
++ D+Y + FS+T +LFRSE ++ ++P
Sbjct: 26 FLVDRYLCDNRFSKTRSLFRSEASSLISNSP 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,842,701
Number of Sequences: 28952
Number of extensions: 387612
Number of successful extensions: 1126
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1086
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1120
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2149324008
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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