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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_B06
         (900 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17A5.13 |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr...   129   6e-31
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c...    28   2.1  
SPCC1393.06c |||rRNA processing protein Ipi1|Schizosaccharomyces...    27   3.6  
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ...    26   6.3  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    26   6.3  
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    26   6.3  
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa...    26   6.3  
SPBC1271.11 |||tricarboxylate transporter |Schizosaccharomyces p...    26   8.4  

>SPAC17A5.13 |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 235

 Score =  129 bits (311), Expect = 6e-31
 Identities = 62/110 (56%), Positives = 78/110 (70%)
 Frame = +1

Query: 562 GRTLKGQGLLKTPERAAKAMLFFTKGYXQSLEEVLNNAIFDEDTDEMVVVKDIEMFSMCE 741
           G   + QGLL TPER AKAML+FTKGY Q+L EV+N A+F ED +EMV+V+DI++FS+CE
Sbjct: 67  GEDPERQGLLGTPERYAKAMLYFTKGYEQNLTEVINEAVFQEDHEEMVIVRDIDVFSLCE 126

Query: 742 HHLVPFYGKVSIGYLPQGXILGLSNWPGSWKYSPAGLQVXXRLTXXIAIA 891
           HHLVPF GK+ IGY+P+  +LGLS            LQV  RLT  +A A
Sbjct: 127 HHLVPFIGKIHIGYIPRKRVLGLSKLARIANMFSRRLQVQERLTKQVAQA 176



 Score = 26.2 bits (55), Expect = 6.3
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +3

Query: 507 EALLPDMANSYRLLLTGLGEDPERAGAL 590
           E  +  ++N+   +L  LGEDPER G L
Sbjct: 49  EEKVKKISNAISTILECLGEDPERQGLL 76


>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 521

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 16/81 (19%), Positives = 31/81 (38%), Gaps = 1/81 (1%)
 Frame = +3

Query: 333 VGKYKKAPEALKSFNLADAE-SELEVPGTPMTPRTSTTPGHENCTFHHDLELDHRPPTRE 509
           +GK     +  K   + +A+  ++    +P    +    GH    FHH++ +   P    
Sbjct: 212 IGKPYIKVDPKKVIGIVEADYGDITCANSPQDETSQAIAGHLVDFFHHEVSVGRLPKNLH 271

Query: 510 ALLPDMANSYRLLLTGLGEDP 572
            L   + N    ++ GL   P
Sbjct: 272 PLQSGIGNIANAIIGGLAHSP 292


>SPCC1393.06c |||rRNA processing protein Ipi1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 408

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = -2

Query: 659 SSKLXSYPFVKKSIALAARSGVFKSPCPFRVL 564
           SS    YP++++ I +  +   F+SPC F  L
Sbjct: 244 SSVHLKYPYLQQCIVIHPKYEAFRSPCSFAYL 275


>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1854

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 19/70 (27%), Positives = 31/70 (44%), Gaps = 4/70 (5%)
 Frame = -2

Query: 380  QVKALQGFWSFFVFTNERALLRSVTVLFLLGYRITYGSRTDATY----QRRWRHCRFRVQ 213
            Q  A  G   FFV  N  +++  +T LF+      Y  RT + +    QR W   R +++
Sbjct: 1182 QTNASSGNAMFFVLFNLVSMIYILT-LFIAIIISNYAERTGSAFFTAEQRAWLELRRKIK 1240

Query: 212  IFSVXRRPCL 183
                 +RP +
Sbjct: 1241 SMRPSKRPAI 1250


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1485

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +3

Query: 321  KGSFVGKYKKAPEALKSFNLADAESELEVPGTPMTPRTSTT 443
            K S   K KKA  ++K  +  D + +  +PG+  TP+ S+T
Sbjct: 1384 KASAKNKGKKA-SSVKKQSPEDDDDDFIIPGSSSTPKASST 1423


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 13/27 (48%), Positives = 17/27 (62%)
 Frame = +1

Query: 640  YXQSLEEVLNNAIFDEDTDEMVVVKDI 720
            Y +SL  +LNN I D  T+E  V KD+
Sbjct: 3007 YYKSLVWMLNNDITDIITEEFAVEKDV 3033


>SPAC23C4.19 |spt5||transcription elongation factor
           Spt5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 990

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 15/46 (32%), Positives = 20/46 (43%), Gaps = 1/46 (2%)
 Frame = +1

Query: 427 RGLRPRLV-TRTAPSTMTWNWTTDRPPGRRCYRTWLTHTGFYSPAW 561
           RG+RP  + T   P+   W      P      RT   +TG  +PAW
Sbjct: 794 RGIRPGSISTADGPNVPNWAQGARTPAVANGSRTPAWNTGSRTPAW 839


>SPBC1271.11 |||tricarboxylate transporter |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 258

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = -1

Query: 693 RVFIEDGVVQNLFQALXVSFREEEHSFGGTFGRLQEPLPFQ 571
           R  I    V  +FQ++  ++R+  HSF G  GR      FQ
Sbjct: 110 RAQISKTSVSQIFQSMIHNYRDLWHSFKGMCGRNVPATAFQ 150


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,504,491
Number of Sequences: 5004
Number of extensions: 73630
Number of successful extensions: 211
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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