Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_A15
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 194 2e-50
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 193 2e-50
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 193 2e-50
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 91 2e-19
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 79 8e-16
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 194 bits (472), Expect = 2e-50
Identities = 106/237 (44%), Positives = 123/237 (51%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L CGG R E F A G GSF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE VT DAPG F G SQA CAVL + GTG FEAGISK+
Sbjct: 71 ITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLA+ LGV L V V M +T +S F S++ G +P V F
Sbjct: 131 GQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPF 188
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VP+SG+ G +EP+T MPW+ GW L+E +D+I P RP +KP R
Sbjct: 189 VPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLR 245
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 193 bits (471), Expect = 2e-50
Identities = 105/237 (44%), Positives = 123/237 (51%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L CGG R E F A G GSF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE VT DAPG F G SQA CA+L + GTG FEAGISK+
Sbjct: 71 ITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLA+ LGV L V V M +T +S F S++ G +P V F
Sbjct: 131 GQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPF 188
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VP+SG+ G +EP+T MPW+ GW L+E +D+I P RP +KP R
Sbjct: 189 VPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLR 245
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 193 bits (471), Expect = 2e-50
Identities = 105/237 (44%), Positives = 123/237 (51%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L CGG R E F A G GSF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE VT DAPG F G SQA CA+L + GTG FEAGISK+
Sbjct: 71 ITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLA+ LGV L V V M +T +S F S++ G +P V F
Sbjct: 131 GQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPF 188
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VP+SG+ G +EP+T MPW+ GW L+E +D+I P RP +KP R
Sbjct: 189 VPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLR 245
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 90.6 bits (215), Expect = 2e-19
Identities = 74/245 (30%), Positives = 93/245 (37%), Gaps = 3/245 (1%)
Frame = +2
Query: 128 PXXXXVVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKA 307
P VV G V SG S G + G R + A G GSF AW+L +
Sbjct: 176 PVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEE 235
Query: 308 XRXRGXXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEA 487
R RG A FE DAPG F G S A AVL V + FE
Sbjct: 236 ERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFER 295
Query: 488 GISKNGXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSY-XXXXGXH 664
G +NG TR A L LG+ + V V + +S F VS + G
Sbjct: 296 GFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFK 353
Query: 665 PAAVXFVPISGWXGXXXL--EPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPX 838
+ V FVPIS G + + S W+ G P L+ LD ++ P +P
Sbjct: 354 TSNVHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPPEKPY 401
Query: 839 EKPRR 853
KP R
Sbjct: 402 RKPLR 406
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 79.0 bits (186), Expect = 8e-16
Identities = 55/207 (26%), Positives = 81/207 (39%), Gaps = 4/207 (1%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
V IG V +G S G + G R E A G S+ +W L R +G
Sbjct: 242 VFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKG 301
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
FE + DAPG + G SQA VL ++A G FEAG +
Sbjct: 302 KTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERG 361
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAA--V 676
G TR A+LA G+ L V + M +S + +S + + + V
Sbjct: 362 GQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGYNSKTDV 421
Query: 677 XFVPISGWXGXXXLE--PSTXMPWFXG 751
++P+S + G + S+ PW+ G
Sbjct: 422 KYMPVSAYTGQNVKDRVDSSVCPWYQG 448
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,322,301
Number of Sequences: 5004
Number of extensions: 11074
Number of successful extensions: 18
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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