Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP02_F_A15
(857 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 169 3e-42
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 169 3e-42
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 169 3e-42
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 169 3e-42
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 81 7e-16
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 73 2e-13
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 169 bits (410), Expect = 3e-42
Identities = 102/237 (43%), Positives = 116/237 (48%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L GG R E F A SF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE T DAPG F G SQA CAVL + + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLAF LGV + M +T P YS + VSSY G +P + F
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPF 190
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VPISG+ G +E ST + W+ G P L+E LD I P RP +KP R
Sbjct: 191 VPISGFEGDNMIERSTNLDWYKG------------PTLLEALDQINEPKRPSDKPLR 235
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 169 bits (410), Expect = 3e-42
Identities = 102/237 (43%), Positives = 116/237 (48%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L GG R E F A SF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE T DAPG F G SQA CAVL + + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLAF LGV + M +T P YS + VSSY G +P + F
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPF 190
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VPISG+ G +E ST + W+ G P L+E LD I P RP +KP R
Sbjct: 191 VPISGFEGDNMIERSTNLDWYKG------------PTLLEALDQINEPKRPSDKPLR 235
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 169 bits (410), Expect = 3e-42
Identities = 102/237 (43%), Positives = 116/237 (48%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L GG R E F A SF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE T DAPG F G SQA CAVL + + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLAF LGV + M +T P YS + VSSY G +P + F
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPF 190
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VPISG+ G +E ST + W+ G P L+E LD I P RP +KP R
Sbjct: 191 VPISGFEGDNMIERSTNLDWYKG------------PTLLEALDQINEPKRPSDKPLR 235
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 169 bits (410), Expect = 3e-42
Identities = 102/237 (43%), Positives = 116/237 (48%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
VVIG V SG S G L GG R E F A SF AWVL LKA R RG
Sbjct: 11 VVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERG 70
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
ALW FE T DAPG F G SQA CAVL + + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR ALLAF LGV + M +T P YS + VSSY G +P + F
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPF 190
Query: 683 VPISGWXGXXXLEPSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
VPISG+ G +E ST + W+ G P L+E LD I P RP +KP R
Sbjct: 191 VPISGFEGDNMIERSTNLDWYKG------------PTLLEALDQINEPKRPSDKPLR 235
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 81.4 bits (192), Expect = 7e-16
Identities = 64/238 (26%), Positives = 90/238 (37%), Gaps = 4/238 (1%)
Frame = +2
Query: 146 VIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRGX 325
++G V SG S G L G + + A G GSF AW L R RG
Sbjct: 244 IVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAEERERGI 303
Query: 326 XXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISK-N 502
A+ F V D+PG F G +QA A+L + A G FEAG
Sbjct: 304 TMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNLK 363
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAAVXF 682
G TR A + GV + V + M YS F V S+ +++ +
Sbjct: 364 GQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFKDSSLTW 421
Query: 683 VPISGWXGXXXL-EPS--TXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKP 847
+P+S + PS W+ G PCL++ +D++ P R KP
Sbjct: 422 IPLSAMENQNLVAAPSDNRLSSWYQG------------PCLLDAVDSVKSPDRDVSKP 467
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 72.9 bits (171), Expect = 2e-13
Identities = 65/240 (27%), Positives = 88/240 (36%), Gaps = 3/240 (1%)
Frame = +2
Query: 143 VVIGXVXSGXSXXXGXLXXXCGGXXXRXXEXFXXXAXXXGXGSFXXAWVLXXLKAXRXRG 322
V IG V +G S G + G R + + A S+ A+++ + R +G
Sbjct: 105 VFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTNEEERLKG 164
Query: 323 XXXXXALWXFEXXXXXVTXXDAPGXXXFXXXXXXGXSQAXCAVLXVAAGTGXFEAGISKN 502
FE T DAPG + G SQA VL ++A G FE G +
Sbjct: 165 KTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERG 224
Query: 503 GXTRXXALLAFXLGVXXLXVGVXXMXSTEPPYSXPXFXXXXXXVSSYXXXXGXHPAA-VX 679
G TR LA LGV L V V M +S + + + G + V
Sbjct: 225 GQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVV 284
Query: 680 FVPISGWXGXXXLE--PSTXMPWFXGWXVXRXXXQADXPCLIEXLDAILXPGRPXEKPRR 853
F+PISG G + PW+ G P E LD+I P R P R
Sbjct: 285 FLPISGLMGKNMDQRMGQEICPWWSG------------PSFFEVLDSIEIPPRDPNGPFR 332
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,621,375
Number of Sequences: 28952
Number of extensions: 70004
Number of successful extensions: 55
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 54
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 54
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1999652000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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