BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_P14
(982 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.19
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.33
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 23 0.53
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 4.0
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 5.3
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 7.1
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.5 bits (68), Expect = 0.19
Identities = 20/75 (26%), Positives = 22/75 (29%)
Frame = +2
Query: 749 PPPPPPXHQXNXXXXXXXXXXXXXXXNQXXPPXXXXXXXXXXXXXXXRPXPPXHKQXXPP 928
PPPPPP + N PP P PP + PP
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSI-------PLPPQGRSAPPP 363
Query: 929 XPPPPXXGGGRXPXP 973
PP GR P P
Sbjct: 364 PPPRSAPSTGRQPPP 378
Score = 25.8 bits (54), Expect = 9.3
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +2
Query: 923 PPXPPPPXXGGGRXPXPP 976
PP PPP G P PP
Sbjct: 338 PPPPPPRSNAAGSIPLPP 355
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.33
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = +2
Query: 899 PPXHKQXXPPXPPPPXXGGGRXPXPP 976
PP PP PPPP G P PP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 30.3 bits (65), Expect = 0.43
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPPXXGGGRXPXPP 976
P P PP PPPP G P PP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 29.5 bits (63), Expect = 0.76
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 508 PPPXGXGGXXXXPXPPXPPTKKXG 579
PPP G G P PP PP G
Sbjct: 765 PPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 29.1 bits (62), Expect = 1.0
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPPXXGGGRXPXPP 976
P P PP PPP G G P PP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 25.0 bits (52), Expect(2) = 1.0
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPP 943
P PP PP PPPP
Sbjct: 765 PPPPGVAGAGPPPPPPP 781
Score = 22.2 bits (45), Expect(2) = 1.0
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2
Query: 923 PPXPPPPXXGGG 958
PP PPPP G
Sbjct: 777 PPPPPPPAVSAG 788
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 23.0 bits (47), Expect(3) = 0.53
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2
Query: 740 PXXPPPPPP 766
P PPPPPP
Sbjct: 21 PSQPPPPPP 29
Score = 21.8 bits (44), Expect(3) = 0.53
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2
Query: 728 PXXXPXXPPPPP 763
P P PPPPP
Sbjct: 5 PPGNPPPPPPPP 16
Score = 21.4 bits (43), Expect(3) = 0.53
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = +2
Query: 749 PPPPPPXH 772
PPPPPP +
Sbjct: 25 PPPPPPGY 32
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 27.1 bits (57), Expect = 4.0
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +3
Query: 750 PPPPPQXTXKTXPPXHXXGXAPPP 821
PPPPP K PP A PP
Sbjct: 1883 PPPPPMALPKAGPPSAAPTSALPP 1906
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.6 bits (56), Expect = 5.3
Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976
P PP H + PPPP G P PP
Sbjct: 221 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 250
Score = 26.6 bits (56), Expect = 5.3
Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976
P PP H + PPPP G P PP
Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 276
Score = 26.6 bits (56), Expect = 5.3
Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976
P PP H + PPPP G P PP
Sbjct: 273 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 302
Score = 26.6 bits (56), Expect = 5.3
Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976
P PP H + PPPP G P PP
Sbjct: 299 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 328
Score = 26.2 bits (55), Expect = 7.1
Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976
P PP H + PPPP G P PP
Sbjct: 208 PPPPMHHKPGEHMPPPPMHHEPGEHMPPPP 237
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 7.1
Identities = 11/28 (39%), Positives = 11/28 (39%)
Frame = +2
Query: 893 PXPPXHKQXXPPXPPPPXXGGGRXPXPP 976
P PP PP PP G P PP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.310 0.144 0.473
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,839,255
Number of Sequences: 5004
Number of extensions: 23904
Number of successful extensions: 157
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 119
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 505288058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.6 bits)
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