BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_P14 (982 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.19 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.33 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 23 0.53 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 4.0 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 5.3 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 7.1 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 31.5 bits (68), Expect = 0.19 Identities = 20/75 (26%), Positives = 22/75 (29%) Frame = +2 Query: 749 PPPPPPXHQXNXXXXXXXXXXXXXXXNQXXPPXXXXXXXXXXXXXXXRPXPPXHKQXXPP 928 PPPPPP + N PP P PP + PP Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSI-------PLPPQGRSAPPP 363 Query: 929 XPPPPXXGGGRXPXP 973 PP GR P P Sbjct: 364 PPPRSAPSTGRQPPP 378 Score = 25.8 bits (54), Expect = 9.3 Identities = 9/18 (50%), Positives = 9/18 (50%) Frame = +2 Query: 923 PPXPPPPXXGGGRXPXPP 976 PP PPP G P PP Sbjct: 338 PPPPPPRSNAAGSIPLPP 355 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 30.7 bits (66), Expect = 0.33 Identities = 12/26 (46%), Positives = 12/26 (46%) Frame = +2 Query: 899 PPXHKQXXPPXPPPPXXGGGRXPXPP 976 PP PP PPPP G P PP Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPP 778 Score = 30.3 bits (65), Expect = 0.43 Identities = 12/28 (42%), Positives = 12/28 (42%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPPXXGGGRXPXPP 976 P P PP PPPP G P PP Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPP 779 Score = 29.5 bits (63), Expect = 0.76 Identities = 11/24 (45%), Positives = 11/24 (45%) Frame = +1 Query: 508 PPPXGXGGXXXXPXPPXPPTKKXG 579 PPP G G P PP PP G Sbjct: 765 PPPPGVAGAGPPPPPPPPPAVSAG 788 Score = 29.1 bits (62), Expect = 1.0 Identities = 12/28 (42%), Positives = 12/28 (42%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPPXXGGGRXPXPP 976 P P PP PPP G G P PP Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPP 780 Score = 25.0 bits (52), Expect(2) = 1.0 Identities = 9/17 (52%), Positives = 9/17 (52%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPP 943 P PP PP PPPP Sbjct: 765 PPPPGVAGAGPPPPPPP 781 Score = 22.2 bits (45), Expect(2) = 1.0 Identities = 7/12 (58%), Positives = 7/12 (58%) Frame = +2 Query: 923 PPXPPPPXXGGG 958 PP PPPP G Sbjct: 777 PPPPPPPAVSAG 788 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 23.0 bits (47), Expect(3) = 0.53 Identities = 7/9 (77%), Positives = 7/9 (77%) Frame = +2 Query: 740 PXXPPPPPP 766 P PPPPPP Sbjct: 21 PSQPPPPPP 29 Score = 21.8 bits (44), Expect(3) = 0.53 Identities = 7/12 (58%), Positives = 7/12 (58%) Frame = +2 Query: 728 PXXXPXXPPPPP 763 P P PPPPP Sbjct: 5 PPGNPPPPPPPP 16 Score = 21.4 bits (43), Expect(3) = 0.53 Identities = 6/8 (75%), Positives = 7/8 (87%) Frame = +2 Query: 749 PPPPPPXH 772 PPPPPP + Sbjct: 25 PPPPPPGY 32 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 27.1 bits (57), Expect = 4.0 Identities = 11/24 (45%), Positives = 11/24 (45%) Frame = +3 Query: 750 PPPPPQXTXKTXPPXHXXGXAPPP 821 PPPPP K PP A PP Sbjct: 1883 PPPPPMALPKAGPPSAAPTSALPP 1906 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 26.6 bits (56), Expect = 5.3 Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976 P PP H + PPPP G P PP Sbjct: 221 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 250 Score = 26.6 bits (56), Expect = 5.3 Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976 P PP H + PPPP G P PP Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 276 Score = 26.6 bits (56), Expect = 5.3 Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976 P PP H + PPPP G P PP Sbjct: 273 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 302 Score = 26.6 bits (56), Expect = 5.3 Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976 P PP H + PPPP G P PP Sbjct: 299 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 328 Score = 26.2 bits (55), Expect = 7.1 Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 2/30 (6%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPP--XXGGGRXPXPP 976 P PP H + PPPP G P PP Sbjct: 208 PPPPMHHKPGEHMPPPPMHHEPGEHMPPPP 237 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.2 bits (55), Expect = 7.1 Identities = 11/28 (39%), Positives = 11/28 (39%) Frame = +2 Query: 893 PXPPXHKQXXPPXPPPPXXGGGRXPXPP 976 P PP PP PP G P PP Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.310 0.144 0.473 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,839,255 Number of Sequences: 5004 Number of extensions: 23904 Number of successful extensions: 157 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 37 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 505288058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.6 bits)
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