Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_O20
(963 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.10
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.18
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 31 0.32
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 1.7
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 2.3
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 2.3
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.0
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.9
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 3.9
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 27 3.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 5.2
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 9.1
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 26 9.1
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.3 bits (70), Expect = 0.10
Identities = 28/117 (23%), Positives = 30/117 (25%), Gaps = 2/117 (1%)
Frame = +2
Query: 614 PXXXPXPRXYPXXRXTLPPXXXXXXPPXPXXTIXXXXXXXXXLXPXXPXKKKXPXXLXPX 793
P P PR LPP PP P + L P P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSN--PPAPPPA 394
Query: 794 PXXXXGPKXXTVXTXXXXXXPPXPXXXPXXPXXPXXXPXPXXPPXXP--PPXXPPXP 958
P + PP P P P P P PP P P PP P
Sbjct: 395 IPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLP-PSAPPSLPMGAPAAPPLP 450
Score = 30.7 bits (66), Expect = 0.32
Identities = 20/63 (31%), Positives = 20/63 (31%), Gaps = 2/63 (3%)
Frame = +1
Query: 781 SXPPS-PXXLXXQXXNXXNXMXSXXPXXPPXXXXGXPXXXXXPXPPPXPPPXP-PXXXPX 954
S PPS P L P PP P P PP PP P P P
Sbjct: 422 SLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPA 481
Query: 955 PXP 963
P P
Sbjct: 482 PAP 484
Score = 28.3 bits (60), Expect = 1.7
Identities = 12/37 (32%), Positives = 12/37 (32%)
Frame = +1
Query: 853 PXXPPXXXXGXPXXXXXPXPPPXPPPXPPXXXPXPXP 963
P PP P PP PP PP P P
Sbjct: 401 PALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPP 437
Score = 27.9 bits (59), Expect = 2.3
Identities = 24/108 (22%), Positives = 25/108 (23%)
Frame = +3
Query: 627 PLPEXTXRXGXXXPXXXRXPPPXXPXXPSPXPXXTPPPFXPSXXXKKXXXXFXAPXPXST 806
P P P R PP P +P PPP S AP P
Sbjct: 341 PPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNP----PAPPPAIP 396
Query: 807 XXPXXXX*XLNXXXXPXXXPXXXPXXPXXXXPPXXPXXPPXXPPXXXP 950
L P P PP P P P P
Sbjct: 397 GRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAP 444
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.18
Identities = 14/32 (43%), Positives = 14/32 (43%)
Frame = +2
Query: 854 PPXPXXXPXXPXXPXXXPXPXXPPXXPPPXXP 949
PP P P P P P P PP PPP P
Sbjct: 1708 PPPPMSVPPPPSAP---PMPAGPPSAPPPPLP 1736
Score = 27.1 bits (57), Expect = 3.9
Identities = 13/36 (36%), Positives = 13/36 (36%), Gaps = 2/36 (5%)
Frame = +2
Query: 857 PXPXXXPXXPXXPXXXPXPXXPPXXP--PPXXPPXP 958
P P P P P P P P PP PP P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 26.6 bits (56), Expect = 5.2
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = +3
Query: 684 PPPXXPXXPSPXPXXTPPPFXPS 752
PPP P P+ P PPP S
Sbjct: 1716 PPPSAPPMPAGPPSAPPPPLPAS 1738
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 30.7 bits (66), Expect = 0.32
Identities = 17/49 (34%), Positives = 27/49 (55%)
Frame = +1
Query: 172 YDPFSPYVRESMLDTHSLWSNLANEMQHLDDMMKELSLKFPSIINEGRV 318
Y+ P ++S + +L S L LDD+ K L+L FP++I + RV
Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRV 199
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.3 bits (60), Expect = 1.7
Identities = 20/68 (29%), Positives = 23/68 (33%)
Frame = +1
Query: 760 QKKXXXXSXPPSPXXLXXQXXNXXNXMXSXXPXXPPXXXXGXPXXXXXPXPPPXPPPXPP 939
Q+K S PP P + + P PP G P PPP PPP
Sbjct: 724 QQKLLLKSPPPPPPAVIVP-----TPAPAPIPVPPPAPIMGGP-------PPPPPPPGVA 771
Query: 940 XXXPXPXP 963
P P P
Sbjct: 772 GAGPPPPP 779
Score = 28.3 bits (60), Expect = 1.7
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +2
Query: 857 PXPXXXPXXPXXPXXXPXPXXPPXXPPPXXPPXP 958
P P P P P P PPP PP P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 23.8 bits (49), Expect(2) = 2.9
Identities = 12/41 (29%), Positives = 16/41 (39%), Gaps = 4/41 (9%)
Frame = +3
Query: 630 LPEXTXRXGXXXPXXXRXPPPXXP----XXPSPXPXXTPPP 740
LP+ + + PPP P P+P P PPP
Sbjct: 714 LPDVSDTVEEQQKLLLKSPPPPPPAVIVPTPAPAPIPVPPP 754
Score = 21.8 bits (44), Expect(2) = 2.9
Identities = 10/34 (29%), Positives = 10/34 (29%)
Frame = +3
Query: 852 PXXXPXXXPXXPXXXXPPXXPXXPPXXPPXXXPP 953
P P P PP P PP PP
Sbjct: 748 PIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.9 bits (59), Expect = 2.3
Identities = 15/53 (28%), Positives = 17/53 (32%)
Frame = +1
Query: 781 SXPPSPXXLXXQXXNXXNXMXSXXPXXPPXXXXGXPXXXXXPXPPPXPPPXPP 939
S PS + + N M S P P PP PPP PP
Sbjct: 145 SQEPSASGVNAPTVSAPNSMVSPPPSFQPPSAAAPATSLPSDYNPPPPPPPPP 197
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.9 bits (59), Expect = 2.3
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 904 PXPPPXPPPXPPXXXPXPXP 963
P PPP PP P P P P
Sbjct: 9 PPPPPPPPGFEPPSQPPPPP 28
Score = 27.5 bits (58), Expect = 3.0
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 893 PXXXPXPXXPPXXPPPXXPPXP 958
P P P PP PP PP P
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPP 27
Score = 26.6 bits (56), Expect = 5.2
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 904 PXPPPXPPPXPPXXXPXPXP 963
P PPP P PP P P P
Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29
Score = 25.8 bits (54), Expect = 9.1
Identities = 10/26 (38%), Positives = 10/26 (38%)
Frame = +2
Query: 863 PXXXPXXPXXPXXXPXPXXPPXXPPP 940
P P P P P PP PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 3.0
Identities = 13/35 (37%), Positives = 13/35 (37%)
Frame = +2
Query: 854 PPXPXXXPXXPXXPXXXPXPXXPPXXPPPXXPPXP 958
PP P P P P PP PP PP P
Sbjct: 1169 PPVPAPSSGIPPVPK--PAAGVPPVPPPSEAPPVP 1201
Score = 26.6 bits (56), Expect = 5.2
Identities = 13/35 (37%), Positives = 13/35 (37%)
Frame = +2
Query: 854 PPXPXXXPXXPXXPXXXPXPXXPPXXPPPXXPPXP 958
PP P P P P PP PP PP P
Sbjct: 1189 PPVP---PPSEAPPVPKPSVGVPPVPPPSTAPPVP 1220
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 3.9
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = -2
Query: 956 GXGXXXGGXGGGXGGGXGXXXXXGXP 879
G G GG GGG GG G G P
Sbjct: 246 GFGGGLGGFGGGPGGFGGGPGGHGGP 271
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.1 bits (57), Expect = 3.9
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 125 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 220
P H+TT R I +I I PS+LT G +C HI
Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 27.1 bits (57), Expect = 3.9
Identities = 16/65 (24%), Positives = 31/65 (47%)
Frame = +1
Query: 199 ESMLDTHSLWSNLANEMQHLDDMMKELSLKFPSIINEGRVEGDKYQISIHLPGYEQKDIN 378
E + + +W+++ +MQ D ++L F I N G++ Q +I L G + ++
Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGKLR-TLLQNAIVLLGQQTTNVA 162
Query: 379 VKAKN 393
K N
Sbjct: 163 SKKLN 167
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 5.2
Identities = 17/52 (32%), Positives = 19/52 (36%), Gaps = 6/52 (11%)
Frame = +3
Query: 627 PLPEXTXRXGXXXPXXXRXPPPXX---PXXPS---PXPXXTPPPFXPSXXXK 764
P P+ R P PPP P PS P P PPP P+ K
Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVK 182
Score = 26.2 bits (55), Expect = 6.9
Identities = 22/84 (26%), Positives = 23/84 (27%)
Frame = +3
Query: 687 PPXXPXXPSPXPXXTPPPFXPSXXXKKXXXXFXAPXPXSTXXPXXXX*XLNXXXXPXXXP 866
PP P P+P PP P AP S P L P
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPS--SLPPPAQPAAPV 181
Query: 867 XXXPXXPXXXXPPXXPXXPPXXPP 938
P P P P PP PP
Sbjct: 182 KSPPSAP--SLPSAVPPMPPKVPP 203
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 25.8 bits (54), Expect = 9.1
Identities = 13/37 (35%), Positives = 13/37 (35%)
Frame = -2
Query: 962 GXGXGXXXGGXGGGXGGGXGXXXXXGXPXXXXGGXXG 852
G G G GGG GG G G GG G
Sbjct: 26 GGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSG 62
>SPCC1450.11c |cek1||serine/threonine protein kinase
Cek1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1338
Score = 25.8 bits (54), Expect = 9.1
Identities = 23/77 (29%), Positives = 39/77 (50%), Gaps = 7/77 (9%)
Frame = +1
Query: 217 HSLWSNLANEMQHLDD--MMKELSL-KFPSI-INEGRVEGDKYQISIHLPGY---EQKDI 375
H++ N A E ++D ++K +S F S+ + + R GD + I I + + I
Sbjct: 573 HTIVPNSAPEHPSINDYKILKPISKGAFGSVYLAQKRTTGDYFAIKILKKSNMIAKNQVI 632
Query: 376 NVKAKNGVLMVQANSAF 426
NV+A+ +LM Q S F
Sbjct: 633 NVRAERAILMSQGESPF 649
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,977,333
Number of Sequences: 5004
Number of extensions: 58747
Number of successful extensions: 363
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 266
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 493304942
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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