Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_O12
(922 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 181 7e-46
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 179 2e-45
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 168 4e-42
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 109 3e-24
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 75 9e-14
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 67 1e-11
At5g56820.1 68418.m07090 F-box family protein contains F-box dom... 34 0.12
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 1.1
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.9
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 30 2.5
At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kina... 29 4.3
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 5.7
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 181 bits (440), Expect = 7e-46
Identities = 96/193 (49%), Positives = 125/193 (64%), Gaps = 5/193 (2%)
Frame = +1
Query: 151 VFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 324
V FEE+F DD WE+ WV SE + G++K TAG + D +DKG++TSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAIS 81
Query: 325 RKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICG 504
+F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPDICG
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICG 141
Query: 505 PGTKKVHVIFSYKGKNHLIKKDI---RXQR*CLHTFVHSDCET*XHL*SPH*HEKVESGD 675
TKKVH I +Y NHLIKKD+ Q ++TF+ T L + + ++G
Sbjct: 142 YSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILID---NVEKQTGS 198
Query: 676 LXADWXLXPPKKI 714
L +DW L PPKKI
Sbjct: 199 LYSDWDLLPPKKI 211
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 179 bits (436), Expect = 2e-45
Identities = 97/210 (46%), Positives = 130/210 (61%), Gaps = 6/210 (2%)
Frame = +1
Query: 139 INCDVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 312
++ +V FEEKF +D WE WV S+ + G++K TAG + D +DKG++TSED RF
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Query: 313 YALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGP 492
YA+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGP
Sbjct: 78 YAISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGP 137
Query: 493 DICGPGTKKVHVIFSYKGKNHLIKKDI---RXQR*CLHTFVHSDCET*XHL*SPH*HEKV 663
DICG TKKVH I +Y G NHLIKK++ Q ++TFV T L + +
Sbjct: 138 DICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILID---NVEK 194
Query: 664 ESGDLXADWXLXPPKKI-XTLXPXTXNWDD 750
++G L +DW L P KKI +WDD
Sbjct: 195 QTGSLYSDWDLLPAKKIKDPSAKKPEDWDD 224
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 168 bits (409), Expect = 4e-42
Identities = 88/204 (43%), Positives = 124/204 (60%), Gaps = 3/204 (1%)
Frame = +1
Query: 148 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 321
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 322 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDIC 501
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPDIC
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDIC 146
Query: 502 GPGTKKVHVIFSYKGKNHLIKKDIRXQR*CLHTFVHSDCET*XHL*SPH*HEKVESGDLX 681
G TKK+HVI SY+G+N+ IKKD++ + L+ F +++ E G +
Sbjct: 147 GTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKEREFGSMY 206
Query: 682 ADWXLXPPKKIXTL-XPXTXNWDD 750
DW + PP+KI +WDD
Sbjct: 207 TDWDILPPRKIKVKNAKKPEDWDD 230
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 109 bits (262), Expect = 3e-24
Identities = 53/114 (46%), Positives = 75/114 (65%), Gaps = 2/114 (1%)
Frame = +1
Query: 148 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 321
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 322 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIM 483
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 74.5 bits (175), Expect = 9e-14
Identities = 43/127 (33%), Positives = 70/127 (55%), Gaps = 4/127 (3%)
Frame = +1
Query: 175 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF-KPFSNE 351
D+ ++ W+ S++ E G +K S+ +D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 352 GKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGTKKV 522
+V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG GT KV
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG-GTNKV 149
Query: 523 HVIFSYK 543
H I +K
Sbjct: 150 HFILKHK 156
Score = 28.3 bits (60), Expect = 7.6
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2
Query: 587 DVYTHLYTLIVKPDXTYEVLID 652
D +H+YT I+KPD +L+D
Sbjct: 177 DKLSHVYTAILKPDNEVRILVD 198
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 67.3 bits (157), Expect = 1e-11
Identities = 44/130 (33%), Positives = 69/130 (53%), Gaps = 7/130 (5%)
Frame = +1
Query: 175 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF---KPFS 345
D+ ++ WV SE + G +K S+ DD GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEK----SEGHDDYGLLVSEKAKKYGIVKELDVDEPLN 90
Query: 346 -NEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGT 513
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-AT 148
Query: 514 KKVHVIFSYK 543
KVH I +K
Sbjct: 149 NKVHFILKHK 158
>At5g56820.1 68418.m07090 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 34.3 bits (75), Expect = 0.12
Identities = 29/102 (28%), Positives = 54/102 (52%), Gaps = 6/102 (5%)
Frame = -3
Query: 344 LNG---LNLRERA*NLASSEVF-KPLSSSGSLKNFPAVNLNFPNSFPGC-SLYTQLLSHE 180
LNG L+L + + NL + ++ KPL S +K+ P +++ PNS P C + + + L +
Sbjct: 305 LNGDRILSLLKHSPNLQTLKLNEKPLRS---IKDQPNISVRKPNSVPECLTFHLETLEWQ 361
Query: 179 S-SGNFSSKNTSQFIEDNASKLTTTSTTAFIFDSQYHLLQQK 57
+G K + +I NA +L T + + + S++ Q+K
Sbjct: 362 GYAGRPEDKEIAVYILGNALRLNTATISRYFSSSRFRHHQKK 403
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 1.1
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +1
Query: 367 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 480
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.9
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 367 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 477
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.9 bits (64), Expect = 2.5
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -3
Query: 455 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNL 327
+ F S L K +P + MS V+W KG+ S L+GL L
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGLYL 189
>At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 769
Score = 29.1 bits (62), Expect = 4.3
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 194 LLSHESSGNFSSKNTSQFIEDNASKLTTTS 105
LLSH S+ N TS+ + + S+LTTTS
Sbjct: 218 LLSHNSTINIDDLRTSKAVSRSLSELTTTS 247
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 5.7
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -3
Query: 461 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 360
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,175,598
Number of Sequences: 28952
Number of extensions: 384452
Number of successful extensions: 932
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 881
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 918
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2188225800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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