Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_N18
(1005 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 36 0.009
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.009
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.15
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.0
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.4
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.8
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 27 5.5
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 26 9.6
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 35.9 bits (79), Expect = 0.009
Identities = 18/60 (30%), Positives = 18/60 (30%)
Frame = +3
Query: 819 HLFKXXPXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXPXXPPPPXP 998
H P S P P PP P P P P G P PP P P P
Sbjct: 1685 HPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 29.1 bits (62), Expect = 1.0
Identities = 13/41 (31%), Positives = 14/41 (34%)
Frame = +3
Query: 852 SPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXP 974
+P P P PPPP P P P P P P
Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.9 bits (79), Expect = 0.009
Identities = 18/52 (34%), Positives = 19/52 (36%)
Frame = +3
Query: 837 PXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXPXXPPPP 992
P + P P P P PP P P P P P PP PPPP
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP----PPPP 783
Score = 30.7 bits (66), Expect = 0.34
Identities = 19/56 (33%), Positives = 19/56 (33%), Gaps = 5/56 (8%)
Frame = +3
Query: 846 KLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPP-----XPXXPPPPXP 998
K P P P P P P P P G P PP P PPPP P
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPA--PIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.5 bits (58), Expect = 3.2
Identities = 18/58 (31%), Positives = 19/58 (32%)
Frame = +3
Query: 801 PPXPXXHLFKXXPXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXP 974
PP P + P P P P PPP P G G P P PP P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP--------PPPPPP 783
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.9 bits (69), Expect = 0.15
Identities = 17/46 (36%), Positives = 17/46 (36%)
Frame = -3
Query: 991 GGGGXXGXGGXGXXXXPXXXGXXPGPGXXXXGXXGGGGXXXGXGXG 854
GG G G G G P G PG G GGG G G G
Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPG 266
Score = 29.5 bits (63), Expect = 0.78
Identities = 18/48 (37%), Positives = 18/48 (37%)
Frame = -3
Query: 997 GXGGGGXXGXGGXGXXXXPXXXGXXPGPGXXXXGXXGGGGXXXGXGXG 854
G GG G G G P G GPG G G GG G G G
Sbjct: 212 GFGGEGHHHGGHGGFGGGP--GGFEGGPGGFGGGPGGFGGGLGGFGGG 257
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 1.0
Identities = 31/127 (24%), Positives = 36/127 (28%), Gaps = 8/127 (6%)
Frame = +3
Query: 642 PPPFIXVPLXXPXX-PPLXLKIINXXLVXKPSPXXXQXXXXXXXXAFCXXPXXXPPXPXX 818
P P P+ P PP+ + V KPS PP P
Sbjct: 1077 PAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVP 1136
Query: 819 HLFKXXPXTKLS--PXPXPXXXPPPPXX----PXXXXPGPGXXPXXXGXXXXPXPPXPXX 980
P ++ P P P PP P P P G P P P P
Sbjct: 1137 SGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSE 1196
Query: 981 PPP-PXP 998
PP P P
Sbjct: 1197 APPVPKP 1203
Score = 29.1 bits (62), Expect = 1.0
Identities = 20/73 (27%), Positives = 23/73 (31%), Gaps = 3/73 (4%)
Frame = +3
Query: 789 PXXXPPXPXXHLFKXX---PXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXP 959
P PP P + P + + P P P PP P P P P
Sbjct: 1155 PSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVP-----KPSVGVPP 1209
Query: 960 XPPXPXXPPPPXP 998
PP PP P P
Sbjct: 1210 VPPPSTAPPVPTP 1222
Score = 28.7 bits (61), Expect = 1.4
Identities = 18/70 (25%), Positives = 19/70 (27%)
Frame = +3
Query: 789 PXXXPPXPXXHLFKXXPXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPP 968
P PP P P + S P P P P P P P P
Sbjct: 1037 PSTAPPVPIPTSTPPVPKSS-SGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVP 1095
Query: 969 XPXXPPPPXP 998
P PP P
Sbjct: 1096 KPSVAAPPVP 1105
Score = 27.1 bits (57), Expect = 4.2
Identities = 17/66 (25%), Positives = 20/66 (30%), Gaps = 4/66 (6%)
Frame = +3
Query: 804 PXPXXHLFKXXPXTKLSPXPXPXXX----PPPPXXPXXXXPGPGXXPXXXGXXXXPXPPX 971
P P + P ++ P P P PPP P P G P P P
Sbjct: 1182 PKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPA 1241
Query: 972 PXXPPP 989
P P
Sbjct: 1242 PSSEAP 1247
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.7 bits (61), Expect = 1.4
Identities = 21/70 (30%), Positives = 22/70 (31%), Gaps = 2/70 (2%)
Frame = +3
Query: 789 PXXXPPXPXXHLFKXXPXTKLSPXPXPXXXPP-PPXXPXXXXPG-PGXXPXXXGXXXXPX 962
P PP P P + S P P PP P P P P P
Sbjct: 140 PTSAPPRP-----SIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAV 194
Query: 963 PPXPXXPPPP 992
PP P PPP
Sbjct: 195 PPMPPKVPPP 204
Score = 26.2 bits (55), Expect = 7.3
Identities = 15/52 (28%), Positives = 15/52 (28%)
Frame = +3
Query: 837 PXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXPXXPPPP 992
P P P PP P P P P PP P PPP
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPS--PASAPPIPSKAPPIPSSLPPP 174
Score = 25.8 bits (54), Expect = 9.6
Identities = 18/59 (30%), Positives = 18/59 (30%), Gaps = 5/59 (8%)
Frame = +3
Query: 837 PXTKLSPXPXPXXXPP----PPXXPXXXXPGPGXXPXXXGXXXXP-XPPXPXXPPPPXP 998
P T S P PP PP P P P P P P P PP P
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAP 188
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.8
Identities = 17/66 (25%), Positives = 17/66 (25%)
Frame = +3
Query: 801 PPXPXXHLFKXXPXTKLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXPXX 980
PP P L P P P P P P G P P
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474
Query: 981 PPPPXP 998
PPP P
Sbjct: 475 APPPAP 480
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 26.6 bits (56), Expect = 5.5
Identities = 16/49 (32%), Positives = 17/49 (34%)
Frame = +3
Query: 846 KLSPXPXPXXXPPPPXXPXXXXPGPGXXPXXXGXXXXPXPPXPXXPPPP 992
+L P PP P P P PG P P P P PP P
Sbjct: 96 ELVPEEPLPREPPLPNEPVPEEPLPGEPPLP--DEPVPEEPLPGEPPLP 142
>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1313
Score = 25.8 bits (54), Expect = 9.6
Identities = 18/42 (42%), Positives = 23/42 (54%), Gaps = 5/42 (11%)
Frame = +2
Query: 62 SETCHNSKCIPRX-LLSAALLVCVNAQVSMP----PGYAEKY 172
+ET H+ + R +SAALLVC+N V P P A KY
Sbjct: 102 TETIHDWRMRERLKTVSAALLVCLNIGVDPPDVIKPNPAAKY 143
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,000,484
Number of Sequences: 5004
Number of extensions: 57598
Number of successful extensions: 204
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 521265546
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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