BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_N14 (845 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.36 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.47 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.5 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 5.8 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 30.3 bits (65), Expect = 0.36 Identities = 13/28 (46%), Positives = 13/28 (46%) Frame = +2 Query: 509 PPXXPPXXXXPPPPXXXPPXAXPXXXPP 592 PP PP PPPP PP P PP Sbjct: 5 PPGNPPPP--PPPPGFEPPSQPPPPPPP 30 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 29.9 bits (64), Expect = 0.47 Identities = 12/28 (42%), Positives = 12/28 (42%) Frame = +2 Query: 509 PPXXPPXXXXPPPPXXXPPXAXPXXXPP 592 P PP PPPP P A P PP Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732 Score = 26.2 bits (55), Expect = 5.8 Identities = 15/48 (31%), Positives = 15/48 (31%) Frame = +2 Query: 485 PKKRGXXXPPXXPPXXXXPPPPXXXPPXAXPXXXPPXXGXXPLXEXPP 628 P R P PPPP PP P PP P PP Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPP---PPSAPPMPAGPPSAPPPP 1734 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 27.5 bits (58), Expect = 2.5 Identities = 18/44 (40%), Positives = 18/44 (40%), Gaps = 1/44 (2%) Frame = -1 Query: 647 GGPXXIRGGTLR-GXGPXXXGGXXKGGXXGXXXGGGGVXXXXGG 519 GGP GG G GP GG GG G G GG GG Sbjct: 228 GGPGGFEGGPGGFGGGPGGFGGGL-GGFGGGPGGFGGGPGGHGG 270 Score = 27.5 bits (58), Expect = 2.5 Identities = 15/42 (35%), Positives = 16/42 (38%) Frame = -3 Query: 645 GPXXY*GGXSXRGXXPQXGGXXXGXAXGGXXXGGGGXXKXGG 520 GP + GG G P G G GG GGG GG Sbjct: 229 GPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 26.2 bits (55), Expect = 5.8 Identities = 17/43 (39%), Positives = 18/43 (41%) Frame = -1 Query: 647 GGPXXIRGGTLRGXGPXXXGGXXKGGXXGXXXGGGGVXXXXGG 519 GG RGG G G GG +GG G G GG GG Sbjct: 19 GGFNGGRGGFGGGRGGARGGG--RGGARGGRGGRGGARGGRGG 59 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,144,798 Number of Sequences: 5004 Number of extensions: 28065 Number of successful extensions: 95 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 28 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 65 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 418457710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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