Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_N13
(847 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 69 5e-12
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 65 5e-11
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 62 5e-10
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 41 0.001
At3g28790.1 68416.m03593 expressed protein 37 0.019
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 33 0.32
At3g02540.2 68416.m00243 ubiquitin family protein contains Pfam ... 31 0.73
At3g02540.1 68416.m00242 ubiquitin family protein contains Pfam ... 31 0.73
At3g24550.1 68416.m03083 protein kinase family protein contains ... 31 0.96
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 31 1.3
At4g12490.1 68417.m01974 protease inhibitor/seed storage/lipid t... 30 2.2
At1g30950.1 68414.m03790 unusual floral organ (UFO) / F-box fami... 30 2.2
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 29 3.9
At1g47660.1 68414.m05295 hypothetical protein 29 3.9
At5g13560.1 68418.m01566 expressed protein weak similarity to SP... 29 5.1
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 29 5.1
At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 29 5.1
At5g38560.1 68418.m04662 protein kinase family protein contains ... 28 9.0
At3g19430.1 68416.m02464 late embryogenesis abundant protein-rel... 28 9.0
At2g21940.2 68415.m02607 shikimate kinase, putative similar to s... 28 9.0
At2g21940.1 68415.m02606 shikimate kinase, putative similar to s... 28 9.0
At1g69295.1 68414.m07947 beta-1,3-glucanase-related low similari... 28 9.0
At1g23180.1 68414.m02896 armadillo/beta-catenin repeat family pr... 28 9.0
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 68.5 bits (160), Expect = 5e-12
Identities = 33/61 (54%), Positives = 46/61 (75%), Gaps = 1/61 (1%)
Frame = +2
Query: 269 DALVVRSATQVTKEVLDAGV-KLKVVGRAGAGVDNIDVDSAGKKGVGVIXAPGANALSAC 445
DAL+VRS T+VT+EV +A +LKVVGRAG G+DN+D+ +A + G V+ AP AN ++A
Sbjct: 124 DALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAA 183
Query: 446 E 448
E
Sbjct: 184 E 184
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 65.3 bits (152), Expect = 5e-11
Identities = 33/70 (47%), Positives = 48/70 (68%), Gaps = 1/70 (1%)
Frame = +2
Query: 269 DALVVRSATQVTKEVLDAGV-KLKVVGRAGAGVDNIDVDSAGKKGVGVIXAPGANALSAC 445
DAL+VRS T+V +EV ++ +LKVVGRAG G+DN+D+ +A + G V+ AP AN ++A
Sbjct: 103 DALIVRSGTKVGREVFESSHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAA 162
Query: 446 ELTCTLMLVL 475
E LM +
Sbjct: 163 EHGIALMAAM 172
Score = 28.7 bits (61), Expect = 5.1
Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = +3
Query: 510 LKAGRWXRGSVHWQRXSLXX-TLPILGLGRVXRXVAXXMYAFGMNII 647
+KAG W R + SL TL +LG G+V VA GM +I
Sbjct: 183 VKAGEWKRNK--YVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVI 227
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 62.1 bits (144), Expect = 5e-10
Identities = 31/70 (44%), Positives = 48/70 (68%), Gaps = 1/70 (1%)
Frame = +2
Query: 269 DALVVRSATQVTKEVLDAGV-KLKVVGRAGAGVDNIDVDSAGKKGVGVIXAPGANALSAC 445
DAL+VRS T+V ++V ++ +LKVVGRAG G+DN+D+ +A + G V+ AP AN ++A
Sbjct: 88 DALIVRSGTKVGRDVFESSRGRLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAA 147
Query: 446 ELTCTLMLVL 475
E L+ +
Sbjct: 148 EHGIALLTAM 157
Score = 30.3 bits (65), Expect = 1.7
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +3
Query: 510 LKAGRWXRGSVHWQRXSLXX-TLPILGLGRVXRXVAXXMYAFGMNII 647
+KAG+W R + SL TL +LG G+V VA GM++I
Sbjct: 168 IKAGKWTRNK--YVGVSLVGKTLAVLGFGKVGSEVARRARGLGMHVI 212
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 40.7 bits (91), Expect = 0.001
Identities = 22/70 (31%), Positives = 35/70 (50%), Gaps = 3/70 (4%)
Frame = +2
Query: 275 LVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVIXAPG---ANALSAC 445
+ V Q+ V+ +K++ + G G+D +D+D+A K G+ V P NA S
Sbjct: 97 ICVAMTMQMDSNVISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGTGNAASCS 156
Query: 446 ELTCTLMLVL 475
E+ LML L
Sbjct: 157 EMAIYLMLGL 166
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 36.7 bits (81), Expect = 0.019
Identities = 17/40 (42%), Positives = 22/40 (55%)
Frame = -2
Query: 435 SALAPGAXITPTPFLPAESTSMLSTPAPARPTTFSFTPAS 316
S +P TPTP P ST STP P+ PT + TP++
Sbjct: 271 SGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPST 310
Score = 33.1 bits (72), Expect = 0.24
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = -2
Query: 408 TPTPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 268
TPTP P ST STP P+ PT TPA +T +E+ + ++
Sbjct: 285 TPTPSTPTPSTPTPSTPTPSTPT--PSTPAPSTPAAGKTSEKGSESA 329
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 32.7 bits (71), Expect = 0.32
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 335 KVVGRAGAGVDNIDVDSAGKKGVGVIXAPGANALSACELTCTLML 469
K G +N+DV++A K G+ V PG + EL +L L
Sbjct: 88 KAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSL 132
>At3g02540.2 68416.m00243 ubiquitin family protein contains Pfam
profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N
domain;
Length = 299
Score = 31.5 bits (68), Expect = 0.73
Identities = 28/90 (31%), Positives = 38/90 (42%), Gaps = 2/90 (2%)
Frame = -2
Query: 564 PAXXAASVQSPXPTCQPSGASGSWDPRGEPRTNINVQVSSHALSALAPGAXITPTPFLPA 385
PA AAS S + S + P P+T +V P TPTP P
Sbjct: 79 PASAAASSASAGTSQAKSIPPSTSQPSISPQTPASVSAPVAPAPTRPPPPAPTPTP-APV 137
Query: 384 ESTSMLSTPAPAR-PTTF-SFTPASNTSLV 301
+T ++TP P P T S TPA +++ V
Sbjct: 138 AATETVTTPIPEPVPATISSSTPAPDSAPV 167
>At3g02540.1 68416.m00242 ubiquitin family protein contains Pfam
profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N
domain;
Length = 419
Score = 31.5 bits (68), Expect = 0.73
Identities = 28/90 (31%), Positives = 38/90 (42%), Gaps = 2/90 (2%)
Frame = -2
Query: 564 PAXXAASVQSPXPTCQPSGASGSWDPRGEPRTNINVQVSSHALSALAPGAXITPTPFLPA 385
PA AAS S + S + P P+T +V P TPTP P
Sbjct: 79 PASAAASSASAGTSQAKSIPPSTSQPSISPQTPASVSAPVAPAPTRPPPPAPTPTP-APV 137
Query: 384 ESTSMLSTPAPAR-PTTF-SFTPASNTSLV 301
+T ++TP P P T S TPA +++ V
Sbjct: 138 AATETVTTPIPEPVPATISSSTPAPDSAPV 167
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 31.1 bits (67), Expect = 0.96
Identities = 26/72 (36%), Positives = 29/72 (40%), Gaps = 2/72 (2%)
Frame = -2
Query: 546 SVQSPXPTCQPSGASGSWDPRGEPRTNINVQVSSHAL--SALAPGAXITPTPFLPAESTS 373
+ SP PT PS P P TN S L S P + TP LP S S
Sbjct: 30 AASSPPPTTTPSS------PPPSPSTNSTSPPPSSPLPPSLPPPSPPGSLTPPLPQPSPS 83
Query: 372 MLSTPAPARPTT 337
TP+P PTT
Sbjct: 84 APITPSPPSPTT 95
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 30.7 bits (66), Expect = 1.3
Identities = 24/90 (26%), Positives = 44/90 (48%), Gaps = 7/90 (7%)
Frame = -2
Query: 564 PAXXAASVQSPXPTCQPSGAS-GSWDPRGEPRTNINVQV------SSHALSALAPGAXIT 406
P+ + S+ P+ PS A S P P +++ SS LS+L+P ++
Sbjct: 29 PSSSSPSLSPSPPSSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPS--LS 86
Query: 405 PTPFLPAESTSMLSTPAPARPTTFSFTPAS 316
P+P + S++ S+ +P+ P S +P+S
Sbjct: 87 PSPPSSSPSSAPPSSLSPSSPPPLSLSPSS 116
>At4g12490.1 68417.m01974 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to pEARLI
1 (Accession No. L43080): an Arabidopsis member of a
conserved gene family (PGF95-099), Plant Physiol. 109
(4), 1497 (1995); contains Pfam protease inhibitor/seed
storage/LTP family domain PF00234
Length = 182
Score = 29.9 bits (64), Expect = 2.2
Identities = 16/58 (27%), Positives = 29/58 (50%)
Frame = -2
Query: 513 SGASGSWDPRGEPRTNINVQVSSHALSALAPGAXITPTPFLPAESTSMLSTPAPARPT 340
+G + +P +PR N +V S + + + PTP +P+ S S P+P+ P+
Sbjct: 24 AGTNCRCNPSPKPRPLPNPKVPSPKVPTPSVPSPYVPTPSVPSPSVPTPSVPSPSVPS 81
>At1g30950.1 68414.m03790 unusual floral organ (UFO) / F-box family
protein (FBX1) E3 ubiquitin ligase SCF complex F-box
subunit; almost identical to unusual floral organs
(UFO)GI:4376159 from [Arabidopsis thaliana]
Landsberg-erecta; one amino acid difference
Length = 442
Score = 29.9 bits (64), Expect = 2.2
Identities = 13/47 (27%), Positives = 25/47 (53%)
Frame = -2
Query: 384 ESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRASWLGXSIRS 244
+ST ++ P+ P +++FT +SN+S T ++ W+ I S
Sbjct: 2 DSTVFINNPSLTLPFSYTFTSSSNSSTTTSTTTDSSSGQWMDGRIWS 48
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 29.1 bits (62), Expect = 3.9
Identities = 16/68 (23%), Positives = 30/68 (44%)
Frame = +2
Query: 272 ALVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVIXAPGANALSACEL 451
A+V ++ +++ L++V G+D ID+ +KG+ V P +L
Sbjct: 48 AVVGNASAGADAQLISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADL 107
Query: 452 TCTLMLVL 475
L+L L
Sbjct: 108 AIGLILAL 115
>At1g47660.1 68414.m05295 hypothetical protein
Length = 275
Score = 29.1 bits (62), Expect = 3.9
Identities = 17/53 (32%), Positives = 20/53 (37%)
Frame = -3
Query: 632 EGVHXXGHXAADPAKAKDGEGXXQRXSLPVYRAPXPPASLQAPVEAGTHVASP 474
+GV AA PA+ PV+ PPA V A HVA P
Sbjct: 19 QGVRTIAPRAAPPARPTTPPPARPTTPPPVWPTTPPPAGAPVAVPAAAHVAVP 71
>At5g13560.1 68418.m01566 expressed protein weak similarity to
SP|O42184 Restin (Cytoplasmic linker protein-170)
(CLIP-170) {Gallus gallus}
Length = 679
Score = 28.7 bits (61), Expect = 5.1
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -3
Query: 533 PXPPASLQAPVEAGTHVASPEPTSTCRSARTRSAH-WRPE 417
P P +S+ +P + T SP+P+S+ +A T S +PE
Sbjct: 584 PSPRSSVTSPKSSATSPKSPKPSSSSMNASTESTQTQKPE 623
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 28.7 bits (61), Expect = 5.1
Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 5/85 (5%)
Frame = -2
Query: 549 ASVQSPXPTCQPSGASGSWDPRGEPRT-NIN----VQVSSHALSALAPGAXITPTPFLPA 385
+S +P P P + + P G P + +I+ + V S + +PG+ P+P P
Sbjct: 496 SSPTTPTPGGSPPSSPTTPSPGGSPPSPSISPSPPITVPSPPSTPTSPGSP--PSPSSPT 553
Query: 384 ESTSMLSTPAPARPTTFSFTPASNT 310
S+ + S P P+ P T +P N+
Sbjct: 554 PSSPIPSPPTPSTPPT-PISPGQNS 577
>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
PF05064 Nsp1-like C-terminal region
Length = 739
Score = 28.7 bits (61), Expect = 5.1
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = -2
Query: 417 AXITPTPFLPAESTSMLSTPAPARPTTFSFTPASNTS 307
A TP+ F + S + S+P+P +TF+ + SNTS
Sbjct: 286 AGSTPSLFASSSSGATTSSPSPFGVSTFNSSSTSNTS 322
>At5g38560.1 68418.m04662 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 681
Score = 27.9 bits (59), Expect = 9.0
Identities = 24/90 (26%), Positives = 37/90 (41%), Gaps = 4/90 (4%)
Frame = -2
Query: 564 PAXXAASVQSPXPTCQPSGASGSWDPRGEPRTNINVQVSSHALSALAP---GAXITPTPF 394
P A+S P P ++ + P P+T + S AP P+P
Sbjct: 81 PPTVASSPPPPVVIASPPPSTPATTPPAPPQTVSPPPPPDASPSPPAPTTTNPPPKPSPS 140
Query: 393 LPAESTSMLS-TPAPARPTTFSFTPASNTS 307
P E+ S TP+P +P+ + TP + TS
Sbjct: 141 PPGETPSPPGETPSPPKPSPSTPTPTTTTS 170
>At3g19430.1 68416.m02464 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 559
Score = 27.9 bits (59), Expect = 9.0
Identities = 24/81 (29%), Positives = 32/81 (39%)
Frame = -2
Query: 543 VQSPXPTCQPSGASGSWDPRGEPRTNINVQVSSHALSALAPGAXITPTPFLPAESTSMLS 364
V P PT PS S + P P V S P TPTP +P+ + + +
Sbjct: 115 VSPPPPTPTPSVPSPT-PPVSPPPPTPTPSVPSPTPPVSPPPP--TPTPSVPSPTPPVPT 171
Query: 363 TPAPARPTTFSFTPASNTSLV 301
P P+ P S P + T V
Sbjct: 172 DPMPSPPPPVSPPPPTPTPSV 192
>At2g21940.2 68415.m02607 shikimate kinase, putative similar to
shikimate kinase precursor from Lycopersicon esculentum
[SP|Q00497]; contains Pfam shikimate kinase domain
PF01202
Length = 304
Score = 27.9 bits (59), Expect = 9.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 504 SGSWDPRGEPRTNINVQVSSHALSA 430
S WD RGE TN N +VS ++A
Sbjct: 240 SAIWDERGEAYTNANARVSLENIAA 264
>At2g21940.1 68415.m02606 shikimate kinase, putative similar to
shikimate kinase precursor from Lycopersicon esculentum
[SP|Q00497]; contains Pfam shikimate kinase domain
PF01202
Length = 303
Score = 27.9 bits (59), Expect = 9.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 504 SGSWDPRGEPRTNINVQVSSHALSA 430
S WD RGE TN N +VS ++A
Sbjct: 239 SAIWDERGEAYTNANARVSLENIAA 263
>At1g69295.1 68414.m07947 beta-1,3-glucanase-related low similarity
to elicitor inducible beta-1,3-glucanase NtEIG-E76
[Nicotiana tabacum] GI:11071974
Length = 222
Score = 27.9 bits (59), Expect = 9.0
Identities = 26/78 (33%), Positives = 30/78 (38%), Gaps = 1/78 (1%)
Frame = -2
Query: 537 SPXPTCQPSGA-SGSWDPRGEPRTNINVQVSSHALSALAPGAXITPTPFLPAESTSMLST 361
S TC +GA S S P P T N S + G TPT P T T
Sbjct: 78 SSGATCDFNGAASPSTTP---PSTASNCLTGSSSSGTPTTG---TPTTGTPTSGTPTTGT 131
Query: 360 PAPARPTTFSFTPASNTS 307
P PTT + T + TS
Sbjct: 132 PTTGTPTTGTPTSGTPTS 149
>At1g23180.1 68414.m02896 armadillo/beta-catenin repeat family
protein contains Pfam profile: PF00514
armadillo/beta-catenin-like repeat
Length = 834
Score = 27.9 bits (59), Expect = 9.0
Identities = 21/75 (28%), Positives = 34/75 (45%)
Frame = +2
Query: 251 MEXPNHDALVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVIXAPGAN 430
ME ++ V S KEVLDA V ++V A N+ ++ G+I P +
Sbjct: 544 MESKFYEGPVNGSKADSRKEVLDAAVFSRLVQIAKTASPNLLRNAISVIEFGIISNPNMD 603
Query: 431 ALSACELTCTLMLVL 475
+ + ++T L L L
Sbjct: 604 TIISKDITTVLDLAL 618
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,618,282
Number of Sequences: 28952
Number of extensions: 208349
Number of successful extensions: 808
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 677
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 785
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1960634400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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