BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_M15
(930 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.025
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.71
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.71
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.6
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 28 2.2
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 2.8
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.8
SPBC12C2.08 |dnm1||dynamin Dnm1|Schizosaccharomyces pombe|chr 2|... 27 5.0
SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomy... 26 8.7
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 8.7
SPBC28F2.02 |mep33||mRNA export protein Mep33|Schizosaccharomyce... 26 8.7
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 34.3 bits (75), Expect = 0.025
Identities = 15/28 (53%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = +3
Query: 849 PPAPXXXPPXPPPPPXG--GXXPXXPTP 926
PPAP P PPPPP G G P P P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 29.5 bits (63), Expect = 0.71
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +3
Query: 852 PAPXXXPPXPPPPPXGGXXPXXPTP 926
PAP P PP P GG P P P
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/34 (35%), Positives = 13/34 (38%)
Frame = +1
Query: 826 PPXXXXXPPXPPXXXPXXLPPPLXGGXXRXPPHP 927
PP P P P P P+ GG PP P
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.5 bits (63), Expect = 0.71
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 849 PPAPXXXPPXPPPPPXGGXXPXXPTP 926
PP P P PPPP P P P
Sbjct: 1721 PPMPAGPPSAPPPPLPASSAPSVPNP 1746
Score = 29.1 bits (62), Expect = 0.93
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 849 PPAPXXXPPXPPPPPXGGXXPXXPTP 926
PP P PP P PP P P P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPP 1733
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.71
Identities = 18/52 (34%), Positives = 19/52 (36%), Gaps = 1/52 (1%)
Frame = +1
Query: 775 SAPXXTPGXGSSGPLXXP-PXXXXXPPXPPXXXPXXLPPPLXGGXXRXPPHP 927
SAP P +S P P PP P P PP L G PP P
Sbjct: 399 SAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLP 450
Score = 28.7 bits (61), Expect = 1.2
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 849 PPAPXXXPPXPPPPPXGGXXPXXPTP 926
PP P P PP PP P P P
Sbjct: 457 PPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/53 (30%), Positives = 19/53 (35%), Gaps = 4/53 (7%)
Frame = +1
Query: 781 PXXTPGXGSSGPLXXPPXXXXXPPXPPXXXPXXL---PPPLXGG-XXRXPPHP 927
P P ++G + PP PP PP PPPL PP P
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAP 391
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/52 (30%), Positives = 16/52 (30%)
Frame = +1
Query: 775 SAPXXTPGXGSSGPLXXPPXXXXXPPXPPXXXPXXLPPPLXGGXXRXPPHPA 930
S P P G PP P PP PPL PP PA
Sbjct: 430 SLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPA 481
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = +3
Query: 858 PXXXPPXPPPPPXGGXXPXXPTP 926
P PP PPPPP G P P P
Sbjct: 5 PPGNPP-PPPPPPGFEPPSQPPP 26
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +1
Query: 826 PPXXXXXPPXPPXXXPXXLPPP 891
PP PP PP P PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPP 26
Score = 27.1 bits (57), Expect = 3.8
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +3
Query: 849 PPAPXXXPPXPPPPP 893
PP P PP PPPP
Sbjct: 13 PPPPGFEPPSQPPPP 27
>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
Vps23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 487
Score = 27.9 bits (59), Expect = 2.2
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = +1
Query: 151 VHAFVKRDAPKEDNSLNTLAESAKKTIEELREKVESALAPETVKKNFGT 297
V A +K+D +E S L TIE+L+ K E+ P K F +
Sbjct: 128 VQALIKQDFEREHTSPPELPTKLVNTIEKLKVKEENEAPPVIPAKPFSS 176
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 27.5 bits (58), Expect = 2.8
Identities = 15/57 (26%), Positives = 29/57 (50%)
Frame = -2
Query: 269 GAKADSTFSLNSSIVFFALSASVFRLLSSLGASRLTNACTLARQIANKIISSLFILD 99
G A + F ++ SIV+ A+ ++V + + A ++ NK+ S L++LD
Sbjct: 410 GYMAITKFEVSQSIVYSAIVSAVAEFIRQILAEDQLLLNNFFEELKNKLESDLYLLD 466
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/27 (40%), Positives = 12/27 (44%)
Frame = +2
Query: 794 PAXALRXPSXXPPXPXXXPPXPXXXXP 874
P LR P+ PP P PP P P
Sbjct: 133 PQSELRPPTSAPPRPSIPPPSPASAPP 159
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = +2
Query: 794 PAXALRXPSXXPPXPXXXPPXPXXXXPXA 880
P A PS PP P PP P P A
Sbjct: 184 PPSAPSLPSAVPPMPPKVPPPPLSQAPVA 212
Score = 25.8 bits (54), Expect = 8.7
Identities = 14/51 (27%), Positives = 15/51 (29%)
Frame = +1
Query: 775 SAPXXTPGXGSSGPLXXPPXXXXXPPXPPXXXPXXLPPPLXGGXXRXPPHP 927
S P +P P PP PP P PP PP P
Sbjct: 148 SIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMP 198
>SPBC12C2.08 |dnm1||dynamin Dnm1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 781
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/19 (63%), Positives = 12/19 (63%)
Frame = -3
Query: 100 IFENVFTNRATRIDQLQNL 44
IF NVFT ID LQNL
Sbjct: 399 IFNNVFTTALNSIDPLQNL 417
>SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 496
Score = 25.8 bits (54), Expect = 8.7
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -2
Query: 239 NSSIVFFALSASVFRLLSSLGASRLTNACTLARQIANKIISSL 111
N S+ F L A+ + S +N C LA+++ ++SSL
Sbjct: 121 NPSLCSFTLPATWSEPPLQISLSTSSNGCRLAQRLLRHVVSSL 163
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 25.8 bits (54), Expect = 8.7
Identities = 14/37 (37%), Positives = 15/37 (40%), Gaps = 1/37 (2%)
Frame = -1
Query: 858 GXGGXXXGXGGXXEGXRRAXAGSXXGG-GGRXXXAGG 751
G GG G G G R G G GGR +GG
Sbjct: 27 GFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63
>SPBC28F2.02 |mep33||mRNA export protein Mep33|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 292
Score = 25.8 bits (54), Expect = 8.7
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +3
Query: 285 KLWHDGRQLQRIL*KSEARGSTESL-RSNFLVSKYITPN 398
K W DGR+ RIL ++E R S ++ RSN +Y N
Sbjct: 216 KKWSDGRR-DRILKQAEERRSNRAVGRSNLSGREYFESN 253
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,710,054
Number of Sequences: 5004
Number of extensions: 46137
Number of successful extensions: 299
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 241
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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