BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_L24 (1001 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 64 3e-11 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 56 1e-08 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 50 7e-07 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 46 6e-06 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 46 6e-06 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 45 1e-05 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 31 0.19 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 29 1.0 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.4 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 28 2.4 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 9.6 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 64.1 bits (149), Expect = 3e-11 Identities = 29/74 (39%), Positives = 46/74 (62%) Frame = +2 Query: 170 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAXEMSDSIXXXXXXXXECX 349 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + SD+ + Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDA-TFIKVDVDQLS 61 Query: 350 DIASEYNINSMPTF 391 +IA+E +++MP+F Sbjct: 62 EIAAEAGVHAMPSF 75 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 55.6 bits (128), Expect = 1e-08 Identities = 31/95 (32%), Positives = 50/95 (52%) Frame = +2 Query: 170 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAXEMSDSIXXXXXXXXECX 349 ++ D TR++ DK+ V+DF A WCGPCK + P L++++ E + + Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLS-EQNQKASFIAVNADKFS 78 Query: 350 DIASEYNINSMPTFVFR*EWQETGRILLALTSTNS 454 DIA + + ++PT V + QE RI+ A T S Sbjct: 79 DIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLS 113 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 49.6 bits (113), Expect = 7e-07 Identities = 24/64 (37%), Positives = 35/64 (54%), Gaps = 2/64 (3%) Frame = +2 Query: 215 DKLVVIDFMATWCGPCKMIGPKLDEIAXEM-SDSIXXXXXXXXECXDIASEYNINSMPTF 391 DK++++ F A WCG CK + P+ + A E+ D I E D+ SEY+I PT Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98 Query: 392 -VFR 400 VF+ Sbjct: 99 NVFK 102 Score = 38.3 bits (85), Expect = 0.002 Identities = 18/60 (30%), Positives = 30/60 (50%) Frame = +2 Query: 218 KLVVIDFMATWCGPCKMIGPKLDEIAXEMSDSIXXXXXXXXECXDIASEYNINSMPTFVF 397 K V+++F A WCG CK + P +++A E SD + + +I+ PT +F Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDD-SNVVVAKIDATENDISVSISGFPTIMF 432 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 46.4 bits (105), Expect = 6e-06 Identities = 24/94 (25%), Positives = 44/94 (46%), Gaps = 2/94 (2%) Frame = +2 Query: 122 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAXE 301 VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67 Query: 302 MSD--SIXXXXXXXXECXDIASEYNINSMPTFVF 397 D + D+A +Y+I PT ++ Sbjct: 68 FEDHNDVLIGKIDADTHSDVADKYHITGFPTLIW 101 Score = 32.3 bits (70), Expect = 0.11 Identities = 16/62 (25%), Positives = 27/62 (43%), Gaps = 2/62 (3%) Frame = +2 Query: 218 KLVVIDFMATWCGPCKMIGPKLDEIAXEMSD--SIXXXXXXXXECXDIASEYNINSMPTF 391 K V+++F A WCG CK + P + + + ++ DI + + S PT Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTI 218 Query: 392 VF 397 F Sbjct: 219 KF 220 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 46.4 bits (105), Expect = 6e-06 Identities = 27/77 (35%), Positives = 36/77 (46%), Gaps = 1/77 (1%) Frame = +2 Query: 224 VVIDFMATWCGPCKMIGPKLDEIAXE-MSDSIXXXXXXXXECXDIASEYNINSMPTFVFR 400 + +D A WCGPCK I P ++A + S E IAS + +MPTFVF Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVF- 80 Query: 401 *EWQETGRILLALTSTN 451 E G+ + LT N Sbjct: 81 ---FENGKQIDMLTGAN 94 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 45.2 bits (102), Expect = 1e-05 Identities = 19/79 (24%), Positives = 41/79 (51%) Frame = +2 Query: 158 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAXEMSDSIXXXXXXX 337 MS+ I + + L +++++++F A W PCK + D+ A + +++ Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV-FLKIEA 59 Query: 338 XECXDIASEYNINSMPTFV 394 + DIA +++N++P FV Sbjct: 60 EKFSDIAESFDVNAVPLFV 78 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 31.5 bits (68), Expect = 0.19 Identities = 18/73 (24%), Positives = 31/73 (42%), Gaps = 2/73 (2%) Frame = +2 Query: 176 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAXEMSD--SIXXXXXXXXECX 349 +S + + + G LVV F A WCG CK + P ++A + + + Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNR 94 Query: 350 DIASEYNINSMPT 388 + S+Y + PT Sbjct: 95 AVCSQYQVQGFPT 107 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 29.1 bits (62), Expect = 1.0 Identities = 15/38 (39%), Positives = 17/38 (44%) Frame = +2 Query: 653 PXXTPPPPXTXTXXPLL*SXPPPXPXPXSXXLPLXPAP 766 P TP PP T P + PPP P S + PAP Sbjct: 725 PQVTPAPP-TPAPTPAVKHHPPPPPVRSSISPSMPPAP 761 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 28.7 bits (61), Expect = 1.4 Identities = 13/35 (37%), Positives = 13/35 (37%) Frame = +2 Query: 665 PPPPXTXTXXPLL*SXPPPXPXPXSXXLPLXPAPP 769 PPPP P P P P P P P PP Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768 Score = 27.1 bits (57), Expect = 4.1 Identities = 12/39 (30%), Positives = 14/39 (35%) Frame = +2 Query: 653 PXXTPPPPXTXTXXPLL*SXPPPXPXPXSXXLPLXPAPP 769 P P P P++ PPP P P P PP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 27.9 bits (59), Expect = 2.4 Identities = 20/92 (21%), Positives = 42/92 (45%), Gaps = 3/92 (3%) Frame = +2 Query: 122 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAXE 301 V+ F+ + + + D+D L++ +++ I + CG CK +GP D + + Sbjct: 15 VVAFITMVQTLVSGVPLTDND-LESEVSKG---TWFIKYYLPSCGACKRLGPMWDNMVEK 70 Query: 302 MSDSIXXXXXXXXE--C-XDIASEYNINSMPT 388 + + E C +++S NI ++PT Sbjct: 71 AKEQVEGSNFHFGEVDCSKELSSCANIRAVPT 102 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 25.8 bits (54), Expect = 9.6 Identities = 17/71 (23%), Positives = 20/71 (28%) Frame = +3 Query: 627 PSXMPTTIGPXXLXPPPPPXLXXHSSNPXXPXPXXXLXXXSPSXRXXXXXXXXXXXXPXX 806 P +P+T P + PP S P P P PS Sbjct: 1033 PIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIP 1092 Query: 807 PPPXPXXRXPP 839 P P P PP Sbjct: 1093 PVPKPSVAAPP 1103 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,467,906 Number of Sequences: 5004 Number of extensions: 41128 Number of successful extensions: 166 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 151 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 519268360 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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