BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_L20 (1054 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.017 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 32 0.12 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.12 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.21 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.83 SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr 2|... 28 2.5 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 4.4 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 35.1 bits (77), Expect = 0.017 Identities = 23/82 (28%), Positives = 26/82 (31%), Gaps = 1/82 (1%) Frame = +3 Query: 525 KKXXPPPPXXXXGXSLLXXTP-PPPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXD 701 K PPP +L PPP P + +N P PPPP Sbjct: 289 KPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAA 348 Query: 702 TSPPPPTQKXGAPQNPQXTKHP 767 S P P Q AP P P Sbjct: 349 GSIPLPPQGRSAPPPPPPRSAP 370 Score = 31.1 bits (67), Expect = 0.27 Identities = 23/78 (29%), Positives = 25/78 (32%), Gaps = 1/78 (1%) Frame = +3 Query: 519 PXKKXXPPPPXXXXGXSLLXXTPPPPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRX 698 P ++ PP G S PPPP P N P PPPP Sbjct: 317 PSRRNRGKPPIGN-GSSNSSLPPPPPPPRSNAAGSIPLP-----PQGRSAPPPPPPRSAP 370 Query: 699 DTS-PPPPTQKXGAPQNP 749 T PPP A NP Sbjct: 371 STGRQPPPLSSSRAVSNP 388 Score = 26.6 bits (56), Expect = 5.8 Identities = 19/77 (24%), Positives = 22/77 (28%), Gaps = 1/77 (1%) Frame = +3 Query: 543 PPXXXXGXSLLXXTPPPPXPXXNXKN-PTXXPQXTXXXXXXXXXXPPPPXXRXDTSPPPP 719 P G + TPP P P + P P PP +PP P Sbjct: 401 PALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLP 460 Query: 720 TQKXGAPQNPQXTKHPP 770 AP P PP Sbjct: 461 AGMPAAPPLPPAAPAPP 477 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 32.3 bits (70), Expect = 0.12 Identities = 23/101 (22%), Positives = 29/101 (28%) Frame = +1 Query: 247 KKKKXGXXXGGGXPPXGGXPPXXKKKXXRXGXXPHQKXRPKKNPXEXKPGXXXKPRXXXP 426 K + G PP PP + + P + P +P P P Sbjct: 111 KLRHIGKSSASAAPPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSS 170 Query: 427 KPXPXXPXPPPRXXXXFFLPPXGGGXXXAXXPKKKXXPPPP 549 P P P P + PP A P PPPP Sbjct: 171 LPPPAQPAAPVKS------PPSAPSLPSAVPPMPPKVPPPP 205 Score = 30.7 bits (66), Expect = 0.36 Identities = 21/86 (24%), Positives = 23/86 (26%) Frame = +3 Query: 519 PXKKXXPPPPXXXXGXSLLXXTPPPPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRX 698 P PP P L T PP P +P P PPP Sbjct: 124 PPSAPAPPTPQS----ELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAA 179 Query: 699 DTSPPPPTQKXGAPQNPQXTKHPPHP 776 PP + P K PP P Sbjct: 180 PVKSPPSAPSLPSAVPPMPPKVPPPP 205 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 32.3 bits (70), Expect = 0.12 Identities = 22/65 (33%), Positives = 23/65 (35%), Gaps = 1/65 (1%) Frame = +3 Query: 585 PPPPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXDTSPPPPTQKXGAPQNPQXT-K 761 PP P P P P PP P T+PP PT G P P T K Sbjct: 1179 PPVPKPAAGVP-PVPPPSEAPPVPKPSVGVPPVPPP--STAPPVPTPSAGLPPVPVPTAK 1235 Query: 762 HPPHP 776 PP P Sbjct: 1236 APPVP 1240 Score = 27.5 bits (58), Expect = 3.3 Identities = 16/59 (27%), Positives = 20/59 (33%) Frame = +3 Query: 591 PPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXDTSPPPPTQKXGAPQNPQXTKHP 767 PP P + K P P + PP ++PP P GAP P P Sbjct: 1012 PPVPKLSSKAPPV-PLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAP 1069 Score = 27.5 bits (58), Expect = 3.3 Identities = 18/64 (28%), Positives = 20/64 (31%) Frame = +3 Query: 585 PPPPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXDTSPPPPTQKXGAPQNPQXTKH 764 PP P P P P PP P PP P G P P ++ Sbjct: 1140 PPVPKPSVAAP-PVPAPSGAPPVPKPSVAAPPVPAPSSGI-PPVPKPAAGVPPVPPPSEA 1197 Query: 765 PPHP 776 PP P Sbjct: 1198 PPVP 1201 Score = 27.1 bits (57), Expect = 4.4 Identities = 17/63 (26%), Positives = 20/63 (31%), Gaps = 1/63 (1%) Frame = +3 Query: 591 PPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXDTSPPPPTQKXGAPQNPQXTKH-P 767 PP P + P P PP P +PP P G P P+ P Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSV-AAPPVPAPSSGIPPVPKPAAGVP 1189 Query: 768 PHP 776 P P Sbjct: 1190 PVP 1192 Score = 26.2 bits (55), Expect = 7.7 Identities = 20/82 (24%), Positives = 24/82 (29%), Gaps = 3/82 (3%) Frame = +3 Query: 540 PPPXXXXGXSLLXXTPP---PPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXDTSP 710 PPP + P PP P + P P PP +P Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPP-VPAPSGAP 1121 Query: 711 PPPTQKXGAPQNPQXTKHPPHP 776 P P AP P + PP P Sbjct: 1122 PVPKPSVAAPPVPVPSGAPPVP 1143 Score = 26.2 bits (55), Expect = 7.7 Identities = 18/64 (28%), Positives = 20/64 (31%) Frame = +3 Query: 585 PPPPXPXXNXKNPTXXPQXTXXXXXXXXXXPPPPXXRXDTSPPPPTQKXGAPQNPQXTKH 764 PP P P P P PP P +PP P AP P + Sbjct: 1102 PPVPKPSVAVP-PVPAPSGAPPVPKPSVAAPPVPVPSG--APPVPKPSVAAPPVPAPSGA 1158 Query: 765 PPHP 776 PP P Sbjct: 1159 PPVP 1162 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 31.5 bits (68), Expect = 0.21 Identities = 17/52 (32%), Positives = 18/52 (34%), Gaps = 2/52 (3%) Frame = +3 Query: 570 LLXXTPPPPXPXXNXKNPTXXPQXTXXXXXXXXX--XPPPPXXRXDTSPPPP 719 LL +PPPP P P P PPPP PPPP Sbjct: 727 LLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778 Score = 26.6 bits (56), Expect = 5.8 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +3 Query: 537 PPPPXXXXGXSLLXXTPPPPXP 602 PPPP G + PPPP P Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPP 782 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 29.5 bits (63), Expect = 0.83 Identities = 18/52 (34%), Positives = 18/52 (34%) Frame = -2 Query: 546 GGGXXFFFXGXGXXPPPPXGREKKXXXXXGGGXXXXWXGFGGXLSGLXGXAG 391 GGG F G G PP P G G GFGG G G G Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPG 238 >SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 272 Score = 27.9 bits (59), Expect = 2.5 Identities = 12/29 (41%), Positives = 12/29 (41%) Frame = +1 Query: 376 PXEXKPGXXXKPRXXXPKPXPXXPXPPPR 462 P KP KPR P P P PP R Sbjct: 154 PVPKKPSKPSKPRKKVPVSHPLPPTPPSR 182 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 27.1 bits (57), Expect = 4.4 Identities = 12/33 (36%), Positives = 12/33 (36%) Frame = +3 Query: 537 PPPPXXXXGXSLLXXTPPPPXPXXNXKNPTXXP 635 PPPP G PPPP P K P Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.145 0.502 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,597,904 Number of Sequences: 5004 Number of extensions: 23256 Number of successful extensions: 97 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 32 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 73 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 553220522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits)
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