BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_L14
(997 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 33 0.047
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.25
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.58
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 25 0.89
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 1.3
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.8
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 33.5 bits (73), Expect = 0.047
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +1
Query: 484 NPHPXPXXGGXDPPPXPPPXPP 549
NP P P G +PP PPP PP
Sbjct: 8 NPPPPPPPPGFEPPSQPPPPPP 29
Score = 29.1 bits (62), Expect = 1.0
Identities = 14/35 (40%), Positives = 15/35 (42%), Gaps = 2/35 (5%)
Frame = +2
Query: 509 GGXTPXPPPP--RXPQXXXPPPKWGXPSAXKNLSP 607
G P PPPP P PPP G KN +P
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Score = 28.7 bits (61), Expect = 1.3
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +2
Query: 938 PPPXKXPPXXGPPPPP 985
PPP PP PPPPP
Sbjct: 14 PPPGFEPPSQPPPPPP 29
Score = 27.1 bits (57), Expect = 4.1
Identities = 13/30 (43%), Positives = 14/30 (46%)
Frame = +2
Query: 533 PPRXPQXXXPPPKWGXPSAXKNLSPPPXPG 622
PP P PPP + PS PPP PG
Sbjct: 5 PPGNPPPPPPPPGFEPPSQP---PPPPPPG 31
Score = 26.6 bits (56), Expect = 5.4
Identities = 11/22 (50%), Positives = 12/22 (54%), Gaps = 2/22 (9%)
Frame = +2
Query: 935 HPPPXKXPPXXGPP--PPPTXP 994
+PPP PP PP PPP P
Sbjct: 8 NPPPPPPPPGFEPPSQPPPPPP 29
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.1 bits (67), Expect = 0.25
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +1
Query: 487 PHPXPXXGGXDPPPXPPPXP 546
P P P G PPP PPP P
Sbjct: 764 PPPPPGVAGAGPPPPPPPPP 783
Score = 30.7 bits (66), Expect = 0.33
Identities = 15/33 (45%), Positives = 15/33 (45%), Gaps = 6/33 (18%)
Frame = +1
Query: 487 PHPXPXXGGXDPPPXPP------PXPPXXXTPA 567
P P P GG PPP PP P PP PA
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPA 784
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/22 (54%), Positives = 12/22 (54%), Gaps = 3/22 (13%)
Frame = +2
Query: 938 PPPXKXPPXX---GPPPPPTXP 994
PPP PP GPPPPP P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPP 782
Score = 28.3 bits (60), Expect = 1.8
Identities = 13/33 (39%), Positives = 13/33 (39%)
Frame = +2
Query: 521 PXPPPPRXPQXXXPPPKWGXPSAXKNLSPPPXP 619
P P PP P PPP P PPP P
Sbjct: 748 PIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 27.1 bits (57), Expect = 4.1
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 938 PPPXKXPPXXGPPPPPTXP 994
P P P GPPPPP P
Sbjct: 750 PVPPPAPIMGGPPPPPPPP 768
Score = 26.2 bits (55), Expect = 7.2
Identities = 14/34 (41%), Positives = 14/34 (41%), Gaps = 1/34 (2%)
Frame = +2
Query: 521 PXPPP-PRXPQXXXPPPKWGXPSAXKNLSPPPXP 619
P PPP P PPP G A PPP P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.9 bits (64), Expect = 0.58
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +2
Query: 521 PXPPPPRXPQXXXPPPKWGXPSAXKNLSPPPXP 619
P PPPP + PP G S+ +L PPP P
Sbjct: 311 PPPPPPPSRRNRGKPPI-GNGSSNSSLPPPPPP 342
Score = 29.1 bits (62), Expect = 1.0
Identities = 10/26 (38%), Positives = 12/26 (46%)
Frame = +1
Query: 463 PPLPEKKNPHPXPXXGGXDPPPXPPP 540
P +P + P P PPP PPP
Sbjct: 234 PSIPSSRPPERVPSLSAPAPPPIPPP 259
Score = 28.7 bits (61), Expect = 1.3
Identities = 11/29 (37%), Positives = 13/29 (44%)
Frame = +1
Query: 463 PPLPEKKNPHPXPXXGGXDPPPXPPPXPP 549
PP P ++N P G PPP PP
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPP 342
Score = 27.5 bits (58), Expect = 3.1
Identities = 15/37 (40%), Positives = 15/37 (40%), Gaps = 2/37 (5%)
Frame = +1
Query: 463 PPLPEKKNPHPXPXXGGXDPPPXPP--PXPPXXXTPA 567
PPLP P G PP PP P PP PA
Sbjct: 447 PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPA 483
Score = 26.6 bits (56), Expect = 5.4
Identities = 13/32 (40%), Positives = 14/32 (43%), Gaps = 3/32 (9%)
Frame = +1
Query: 463 PPLPEKKNPH---PXPXXGGXDPPPXPPPXPP 549
PP P + N P P G PPP PP P
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Score = 25.8 bits (54), Expect = 9.5
Identities = 15/41 (36%), Positives = 15/41 (36%), Gaps = 3/41 (7%)
Frame = +2
Query: 509 GGXTPXPPPP-RXPQXXXPPPKWGXPSAXKN--LSPPPXPG 622
G P PPPP P PP A N PP PG
Sbjct: 357 GRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 24.6 bits (51), Expect(2) = 0.89
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = +1
Query: 508 GGXDPPPXPPP 540
GG PPP PPP
Sbjct: 303 GGLPPPPPPPP 313
Score = 23.0 bits (47), Expect(2) = 0.89
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +1
Query: 523 PPXPPPXPP 549
PP PPP PP
Sbjct: 306 PPPPPPPPP 314
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.7 bits (61), Expect = 1.3
Identities = 14/34 (41%), Positives = 16/34 (47%), Gaps = 1/34 (2%)
Frame = +2
Query: 521 PXPPPPRXPQXXXPPPKW-GXPSAXKNLSPPPXP 619
P PPPP P PPP P+ + PPP P
Sbjct: 1705 PTPPPP--PMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 26.6 bits (56), Expect = 5.4
Identities = 10/25 (40%), Positives = 11/25 (44%)
Frame = +1
Query: 472 PEKKNPHPXPXXGGXDPPPXPPPXP 546
P+ P P P PPP PP P
Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMP 1724
Score = 26.2 bits (55), Expect = 7.2
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +2
Query: 938 PPPXKXPPXXGPPPPPTXP 994
PPP PP PP P P
Sbjct: 1709 PPPMSVPPPPSAPPMPAGP 1727
Score = 25.8 bits (54), Expect = 9.5
Identities = 12/35 (34%), Positives = 13/35 (37%)
Frame = +1
Query: 463 PPLPEKKNPHPXPXXGGXDPPPXPPPXPPXXXTPA 567
PP P P P PP PPP P P+
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPS 1742
Score = 25.8 bits (54), Expect = 9.5
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = +2
Query: 929 SXHPPPXKXPPXXGPPPPPTXPI 997
S PPP P GPP P P+
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPL 1735
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.3 bits (60), Expect = 1.8
Identities = 12/35 (34%), Positives = 14/35 (40%)
Frame = +1
Query: 463 PPLPEKKNPHPXPXXGGXDPPPXPPPXPPXXXTPA 567
PP+P P P PP PP P TP+
Sbjct: 1189 PPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPS 1223
Score = 26.6 bits (56), Expect = 5.4
Identities = 13/36 (36%), Positives = 14/36 (38%)
Frame = +2
Query: 512 GXTPXPPPPRXPQXXXPPPKWGXPSAXKNLSPPPXP 619
G P PPP P P P G P + PP P
Sbjct: 1187 GVPPVPPPSEAPPV--PKPSVGVPPVPPPSTAPPVP 1220
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.314 0.146 0.498
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,702,230
Number of Sequences: 5004
Number of extensions: 22464
Number of successful extensions: 294
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 42
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 515273988
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
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