BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_L08 (934 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 31 0.31 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 3.8 SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 27 3.8 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 5.0 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 26 8.7 >SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizosaccharomyces pombe|chr 3|||Manual Length = 640 Score = 30.7 bits (66), Expect = 0.31 Identities = 17/49 (34%), Positives = 27/49 (55%) Frame = +1 Query: 181 YDPFSPYVRESMLDTHSLWSNLANEMQHLDDMMKELSLKFPSIINEGRV 327 Y+ P ++S + +L S L LDD+ K L+L FP++I + RV Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRV 199 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 27.1 bits (57), Expect = 3.8 Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = +2 Query: 134 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 229 P H+TT R I +I I PS+LT G +C HI Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583 >SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 851 Score = 27.1 bits (57), Expect = 3.8 Identities = 16/65 (24%), Positives = 31/65 (47%) Frame = +1 Query: 208 ESMLDTHSLWSNLANEMQHLDDMMKELSLKFPSIINEGRVEGDKYQISIHLPGYEQKDIN 387 E + + +W+++ +MQ D ++L F I N G++ Q +I L G + ++ Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGKLR-TLLQNAIVLLGQQTTNVA 162 Query: 388 VKAKN 402 K N Sbjct: 163 SKKLN 167 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 26.6 bits (56), Expect = 5.0 Identities = 11/31 (35%), Positives = 11/31 (35%) Frame = +2 Query: 689 PPXPXPKXLXKXHPPXPXXXXQKXXPXXPCP 781 PP P P K HPP P P P Sbjct: 731 PPTPAPTPAVKHHPPPPPVRSSISPSMPPAP 761 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 25.8 bits (54), Expect = 8.7 Identities = 23/77 (29%), Positives = 39/77 (50%), Gaps = 7/77 (9%) Frame = +1 Query: 226 HSLWSNLANEMQHLDD--MMKELSL-KFPSI-INEGRVEGDKYQISIHLPGY---EQKDI 384 H++ N A E ++D ++K +S F S+ + + R GD + I I + + I Sbjct: 573 HTIVPNSAPEHPSINDYKILKPISKGAFGSVYLAQKRTTGDYFAIKILKKSNMIAKNQVI 632 Query: 385 NVKAKNGVLMVQANSAF 435 NV+A+ +LM Q S F Sbjct: 633 NVRAERAILMSQGESPF 649 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,592,962 Number of Sequences: 5004 Number of extensions: 49361 Number of successful extensions: 150 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 473333082 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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