BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_K24 (945 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.019 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.31 SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 26 8.9 SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 26 8.9 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 8.9 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 8.9 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 34.7 bits (76), Expect = 0.019 Identities = 29/102 (28%), Positives = 34/102 (33%), Gaps = 2/102 (1%) Frame = +1 Query: 532 RXXXTTGXXPTPXAXGXXXPNXPPPPXRXFFXSFSXPXXPPLSPXXFPXHNQXAXTPLXP 711 R +TG P P + N P PP S P PPL + TP P Sbjct: 367 RSAPSTGRQPPPLSSSRAVSNPPAPPPA--IPGRSAPALPPLG------NASRTSTPPVP 418 Query: 712 PPPXTXFTXXXXLPRLTPSPHPLXXRGAXPGP--XXXXPPXP 831 PP + LP P P+ A P P PP P Sbjct: 419 TPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLP 460 Score = 27.1 bits (57), Expect = 3.8 Identities = 19/65 (29%), Positives = 22/65 (33%), Gaps = 5/65 (7%) Frame = +1 Query: 598 PPPPXRXFFXSFSXPXXPPLSPXXFPXHNQXAXTPLXP-----PPPXTXFTXXXXLPRLT 762 PP P + P PP +P P A PL P PP LP Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPM-GAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAA 473 Query: 763 PSPHP 777 P+P P Sbjct: 474 PAPPP 478 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 30.7 bits (66), Expect = 0.31 Identities = 15/49 (30%), Positives = 18/49 (36%) Frame = +2 Query: 650 RPFXHXPSHXTTXXXPPPXXXPPPXPXSPXXXSSPAXHLPPTPSXXXAP 796 RP P + PPP PP P +P + P PP AP Sbjct: 1693 RPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741 Score = 29.1 bits (62), Expect = 0.95 Identities = 12/36 (33%), Positives = 14/36 (38%) Frame = +2 Query: 695 PPPXXXPPPXPXSPXXXSSPAXHLPPTPSXXXAPXP 802 PPP PPP P P+ PP P+ P Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744 >SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1372 Score = 25.8 bits (54), Expect = 8.9 Identities = 10/19 (52%), Positives = 10/19 (52%) Frame = +2 Query: 695 PPPXXXPPPXPXSPXXXSS 751 PPP PPP P P SS Sbjct: 806 PPPAPLPPPAPPLPTAMSS 824 >SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 25.8 bits (54), Expect = 8.9 Identities = 13/38 (34%), Positives = 16/38 (42%) Frame = +1 Query: 559 PTPXAXGXXXPNXPPPPXRXFFXSFSXPXXPPLSPXXF 672 PTP G P PPP + + P P +SP F Sbjct: 347 PTPQVQGSRPP-PPPPMPAPIYNVPNVPTVPTVSPNPF 383 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 25.8 bits (54), Expect = 8.9 Identities = 14/37 (37%), Positives = 16/37 (43%), Gaps = 3/37 (8%) Frame = +2 Query: 695 PPPXXXPPPXPXSPXXXSSPAXHLP---PTPSXXXAP 796 PP PPP P S S A +P P P+ AP Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAP 180 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.8 bits (54), Expect = 8.9 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +2 Query: 713 PPPXPXSPXXXSSPAXHLPPTP 778 PPP P + S+PA PP P Sbjct: 908 PPPPPTASMTASAPAIASPPPP 929 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,435,587 Number of Sequences: 5004 Number of extensions: 16246 Number of successful extensions: 109 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 36 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 90 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 481321826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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