BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_K24
(945 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.019
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.31
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 26 8.9
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 26 8.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 8.9
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 8.9
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.7 bits (76), Expect = 0.019
Identities = 29/102 (28%), Positives = 34/102 (33%), Gaps = 2/102 (1%)
Frame = +1
Query: 532 RXXXTTGXXPTPXAXGXXXPNXPPPPXRXFFXSFSXPXXPPLSPXXFPXHNQXAXTPLXP 711
R +TG P P + N P PP S P PPL + TP P
Sbjct: 367 RSAPSTGRQPPPLSSSRAVSNPPAPPPA--IPGRSAPALPPLG------NASRTSTPPVP 418
Query: 712 PPPXTXFTXXXXLPRLTPSPHPLXXRGAXPGP--XXXXPPXP 831
PP + LP P P+ A P P PP P
Sbjct: 419 TPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLP 460
Score = 27.1 bits (57), Expect = 3.8
Identities = 19/65 (29%), Positives = 22/65 (33%), Gaps = 5/65 (7%)
Frame = +1
Query: 598 PPPPXRXFFXSFSXPXXPPLSPXXFPXHNQXAXTPLXP-----PPPXTXFTXXXXLPRLT 762
PP P + P PP +P P A PL P PP LP
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPM-GAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAA 473
Query: 763 PSPHP 777
P+P P
Sbjct: 474 PAPPP 478
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.31
Identities = 15/49 (30%), Positives = 18/49 (36%)
Frame = +2
Query: 650 RPFXHXPSHXTTXXXPPPXXXPPPXPXSPXXXSSPAXHLPPTPSXXXAP 796
RP P + PPP PP P +P + P PP AP
Sbjct: 1693 RPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 29.1 bits (62), Expect = 0.95
Identities = 12/36 (33%), Positives = 14/36 (38%)
Frame = +2
Query: 695 PPPXXXPPPXPXSPXXXSSPAXHLPPTPSXXXAPXP 802
PPP PPP P P+ PP P+ P
Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 25.8 bits (54), Expect = 8.9
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 695 PPPXXXPPPXPXSPXXXSS 751
PPP PPP P P SS
Sbjct: 806 PPPAPLPPPAPPLPTAMSS 824
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 25.8 bits (54), Expect = 8.9
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = +1
Query: 559 PTPXAXGXXXPNXPPPPXRXFFXSFSXPXXPPLSPXXF 672
PTP G P PPP + + P P +SP F
Sbjct: 347 PTPQVQGSRPP-PPPPMPAPIYNVPNVPTVPTVSPNPF 383
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/37 (37%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = +2
Query: 695 PPPXXXPPPXPXSPXXXSSPAXHLP---PTPSXXXAP 796
PP PPP P S S A +P P P+ AP
Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAP 180
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.8 bits (54), Expect = 8.9
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2
Query: 713 PPPXPXSPXXXSSPAXHLPPTP 778
PPP P + S+PA PP P
Sbjct: 908 PPPPPTASMTASAPAIASPPPP 929
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,435,587
Number of Sequences: 5004
Number of extensions: 16246
Number of successful extensions: 109
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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